pRetroG-CMV-HA
Vector Name | pRetroG-CMV-HA |
VectorType | Retroviral Plasmid |
Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
Additional Information | The gene expression is driven by a CMV promoter, with a C-terminal HA tag. Original Vector: 8026bp Vector without Insert Cassette: 6321bp |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg 4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt 4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc 4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag 4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag 4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag 4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat 5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga 5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc 5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag 5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc 5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt 5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca 5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa 5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag 5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt 5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc 5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct 7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg 7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc 7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct
LOCUS dna 8026 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4625..4630 /dbxref="REBASE:XhoI" misc_binding 4720..4725 /dbxref="REBASE:ClaI" Other Gene 4807..5358 /gene="5_LTR2 other" misc_binding 5062..5067 /dbxref="REBASE:XbaI" Other Gene 5611..5644 /gene="loxP other" Other Gene 5704..5737 /gene="loxP other" Rep_Origin 6013..6632 /gene="pBR322 origin" Marker 6787..7647 /gene="amp marker" ORF 6787..7647 /sequence="ORF_2 rf(5)" Promoter 7689..7717 /gene="amp prom" BASE COUNT 1938 a 2167 c 2009 g 1912 t 0 others ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg 4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt 4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc 4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag 4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag 4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag 4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat 5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga 5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc 5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag 5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc 5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt 5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca 5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa 5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag 5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt 5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc 5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct 7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg 7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc 7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct //