pRetroG-CMV-HA

Vector Name pRetroG-CMV-HA
VectorType Retroviral Plasmid
Antibiotic Information Bacterial: Ampicillin
Mammalian: Puromycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information The gene expression is driven by a CMV promoter, with a C-terminal HA tag.
Original Vector: 8026bp
Vector without Insert Cassette: 6321bp
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc     
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac     
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg     
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc     
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc     
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc     
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga     
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca     
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc     
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct     
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca     
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg     
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct     
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga     
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg     
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa     
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca     
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac     
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc     
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac     
     2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag     
     2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta     
     3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg     
     3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag     
     3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga     
     3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac     
     3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa     
     3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga     
     3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta     
     3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga     
     3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc     
     3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag     
     3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac     
     3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca     
     3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg     
     3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca     
     3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg     
     3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac     
     3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca     
     4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga     
     4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct     
     4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     
     4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt     
     4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     
     4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa     
     4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     
     4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga     
     4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa     
     4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga     
     4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg     
     4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt     
     4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc     
     4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag     
     4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag     
     4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag     
     4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat     
     5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga     
     5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc     
     5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag     
     5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc     
     5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt     
     5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca     
     5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa     
     5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag     
     5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac     
     5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag     
     5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt     
     5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc     
     5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt     
     5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     
     5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     
     5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     
     6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     
     6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     
     6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     
     6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     
     6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     
     6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     
     6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     
     6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     
     6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     
     6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     
     7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc     
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     
     7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa     
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat     
     7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct     
     7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg     
     7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc     
     7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct                    
                            LOCUS       dna                      8026 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      659..1468
                     /gene="psi_plus_pack other"
     ORF             1449..2216
                     /sequence="ORF_3 rf(6)"
     Marker          1566..2165
                     /gene="puro marker"
     Promoter        2787..2868
                     /gene="CMV prom"
     misc_binding    2894..2899
                     /dbxref="REBASE:HindIII"
     Other Gene      2913..3037
                     /gene="attR1 other"
     Other Gene      2937..3037
                     /gene="attR2 other"
     Marker          3146..3805
                     /gene="CAT marker"
     ORF             3146..3805
                     /sequence="ORF_1 rf(2)"
     Other Gene      4147..4452
                     /gene="ccdB other"
     Other Gene      4493..4612
                     /gene="attR1 other"
     Other Gene      4493..4593
                     /gene="attR2 other"
     misc_binding    4625..4630
                     /dbxref="REBASE:XhoI"
     misc_binding    4720..4725
                     /dbxref="REBASE:ClaI"
     Other Gene      4807..5358
                     /gene="5_LTR2 other"
     misc_binding    5062..5067
                     /dbxref="REBASE:XbaI"
     Other Gene      5611..5644
                     /gene="loxP other"
     Other Gene      5704..5737
                     /gene="loxP other"
     Rep_Origin      6013..6632
                     /gene="pBR322 origin"
     Marker          6787..7647
                     /gene="amp marker"
     ORF             6787..7647
                     /sequence="ORF_2 rf(5)"
     Promoter        7689..7717
                     /gene="amp prom"
BASE COUNT    1938 a   2167 c   2009 g   1912 t    0 others
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc     
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac     
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg     
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc     
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc     
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc     
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga     
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca     
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc     
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct     
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca     
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg     
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct     
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga     
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg     
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa     
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca     
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac     
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc     
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac     
     2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag     
     2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta     
     3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg     
     3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag     
     3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga     
     3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac     
     3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa     
     3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga     
     3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta     
     3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga     
     3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc     
     3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag     
     3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac     
     3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca     
     3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg     
     3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca     
     3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg     
     3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac     
     3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca     
     4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga     
     4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct     
     4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     
     4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt     
     4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     
     4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa     
     4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     
     4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga     
     4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa     
     4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga     
     4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg     
     4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt     
     4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc     
     4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag     
     4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag     
     4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag     
     4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat     
     5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga     
     5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc     
     5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag     
     5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc     
     5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt     
     5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca     
     5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa     
     5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag     
     5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac     
     5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag     
     5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt     
     5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc     
     5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt     
     5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     
     5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     
     5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     
     6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     
     6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     
     6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     
     6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     
     6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     
     6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     
     6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     
     6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     
     6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     
     6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     
     7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc     
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     
     7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa     
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat     
     7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct     
     7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg     
     7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc     
     7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct                    
//