pRetroG-CMV-IRES-GFP

                            LOCUS       dna                      9405 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      659..1468
                     /gene="psi_plus_pack other"
     ORF             1449..2216
                     /sequence="ORF_4 rf(5)"
     Marker          1566..2165
                     /gene="puro marker"
     Promoter        2787..2868
                     /gene="CMV prom"
     Other Gene      2913..3037
                     /gene="attR1 other"
     Other Gene      2937..3037
                     /gene="attR2 other"
     Marker          3146..3805
                     /gene="CAT marker"
     ORF             3146..3805
                     /sequence="ORF_1 rf(2)"
     Other Gene      4147..4452
                     /gene="ccdB other"
     Other Gene      4493..4612
                     /gene="attR1 other"
     Other Gene      4493..4593
                     /gene="attR2 other"
     misc_binding    4628..4633
                     /dbxref="REBASE:XhoI"
     misc_binding    4634..4639
                     /dbxref="REBASE:HpaI"
     Regulatory_Seq  4645..5227
                     /gene="IRES reg"
     Regulatory_Seq  4719..5219
                     /gene="ires_emcv reg"
     ORF             5219..5950
                     /sequence="ORF_2 rf(2)"
     Reporter        5231..5947
                     /gene="EGFP reporter"
     misc_binding    6099..6104
                     /dbxref="REBASE:ClaI"
     Other Gene      6186..6737
                     /gene="5_LTR2 other"
     Other Gene      6990..7023
                     /gene="loxP other"
     Other Gene      7083..7116
                     /gene="loxP other"
     Rep_Origin      7392..8011
                     /gene="pBR322 origin"
     Marker          8166..9026
                     /gene="amp marker"
     ORF             8166..9026
                     /sequence="ORF_3 rf(5)"
     Promoter        9068..9096
                     /gene="amp prom"
BASE COUNT    2269 a   2580 c   2378 g   2178 t    0 others
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc     
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac     
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg     
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc     
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc     
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc     
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga     
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca     
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc     
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct     
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca     
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg     
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct     
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga     
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg     
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa     
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca     
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac     
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc     
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac     
     2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag     
     2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta     
     3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg     
     3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag     
     3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga     
     3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac     
     3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa     
     3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga     
     3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta     
     3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga     
     3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc     
     3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag     
     3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac     
     3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca     
     3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg     
     3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca     
     3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg     
     3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac     
     3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca     
     4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga     
     4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct     
     4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     
     4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt     
     4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     
     4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa     
     4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     
     4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga     
     4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa     
     4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga     
     4621 tatctgactc gaggttaacg aattccctct ccctcccccc cccctaacgt tactggccga     
     4681 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg     
     4741 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg     
     4801 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt     
     4861 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac     
     4921 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca     
     4981 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg     
     5041 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg     
     5101 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa     
     5161 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat     
     5221 ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt     
     5281 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga     
     5341 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc     
     5401 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga     
     5461 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg     
     5521 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg     
     5581 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat     
     5641 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa     
     5701 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt     
     5761 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc     
     5821 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga     
     5881 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct     
     5941 gtacaagtaa tgaattaatt aagaattatc accgcttcta ttcagccagt aaggcctgtc     
     6001 tttaatggcc tccggcatga gacactttct agagggcccg tttaaacgaa ttcgttaggc     
     6061 cattaaggcc tgtcgacaag cggccgcctc ggccaaacat cgataaaata aaagatttta     
     6121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct     
     6181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga     
     6241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc     
     6301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg     
     6361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg     
     6421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac     
     6481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg     
     6541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt     
     6601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca     
     6661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc     
     6721 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac     
     6781 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac     
     6841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc     
     6901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc     
     6961 cagtcacgta gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt     
     7021 tattaggtct gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta     
     7081 atataacttc gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca     
     7141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta     
     7201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg     
     7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca     
     7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     
     7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     
     7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     
     7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     
     7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     
     7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     
     7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     
     7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     
     7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt     
     7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     
     7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     
     7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     
     8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     
     8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     
     8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     
     8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     
     8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     
     8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     
     8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     
     8461 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct     
     8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     
     8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     
     8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     
     8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     
     8761 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa     
     8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     
     8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     
     8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     
     9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     
     9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     
     9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc     
     9181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta     
     9241 gcaattgcta gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt     
     9301 gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc     
     9361 ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgct                     
//