pRetroG-CMV-RFP

                            LOCUS       dna                      6959 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      659..1468
                     /gene="psi_plus_pack other"
     ORF             1460..2215
                     /sequence="ORF_3 rf(5)"
     Marker          1565..2164
                     /gene="puro marker"
     Promoter        2786..2867
                     /gene="CMV prom"
     misc_binding    2887..2892
                     /dbxref="REBASE:BglII"
     misc_binding    2891..2896
                     /dbxref="REBASE:XhoI"
     ORF             2903..3601
                     /sequence="ORF_1 rf(2)"
     misc_binding    3602..3607
                     /dbxref="REBASE:EcoRI"
     misc_binding    3634..3641
                     /dbxref="REBASE:NotI"
     misc_binding    3653..3658
                     /dbxref="REBASE:ClaI"
     Other Gene      3740..4291
                     /gene="5_LTR2 other"
     misc_binding    3995..4000
                     /dbxref="REBASE:XbaI"
     Other Gene      4544..4577
                     /gene="loxP other"
     Other Gene      4637..4670
                     /gene="loxP other"
     Rep_Origin      4946..5565
                     /gene="pBR322 origin"
     Marker          5720..6580
                     /gene="amp marker"
     ORF             5720..6580
                     /sequence="ORF_2 rf(5)"
     Promoter        6622..6650
                     /gene="amp prom"
BASE COUNT    1626 a   2020 c   1792 g   1521 t    0 others
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc     
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc     
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc     
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc     
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct     
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc     
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag     
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa     
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct     
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt     
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac     
     2101 cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca agcccggtgc     
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc     
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac     
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg     
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga     
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat     
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa     
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca     
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca     
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat     
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg     
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac     
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc     
     2881 ggactcagat ctcgaggcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa     
     2941 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg     
     3001 caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc     
     3061 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca     
     3121 cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca catgggagag     
     3181 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccggga     
     3241 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt     
     3301 gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg     
     3361 cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg     
     3421 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt     
     3481 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga     
     3541 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagata     
     3601 agaattcgtt aggccattaa ggcctgtcga caagcggccg cctcggccaa acatcgataa     
     3661 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt     
     3721 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa     
     3781 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga     
     3841 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat     
     3901 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag     
     3961 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag     
     4021 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt     
     4081 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca     
     4141 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc     
     4201 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg     
     4261 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc     
     4321 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg     
     4381 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag     
     4441 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg     
     4501 gcgcagccat gacccagtca cgtagcgata gcggagtgta ctgataactt cgtataatgt     
     4561 atgctatacg aagttattag gtctgaagag gagtttacgt ccagccaagc tagggccgcg     
     4621 atccggaacc cttaatataa cttcgtataa tgtatgctat acgaagttat cagtactggc     
     4681 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac     
     4741 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg     
     4801 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     
     4861 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     
     4921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     
     4981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     
     5041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     
     5101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     
     5161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     
     5221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     
     5281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     
     5341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     
     5401 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     
     5461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     
     5521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     
     5581 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     
     5641 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     
     5701 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     
     5761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     
     5821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     
     5881 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     
     5941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     
     6001 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt     
     6061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     
     6121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     
     6181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     
     6241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     
     6301 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata     
     6361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     
     6421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     
     6481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     
     6541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     
     6601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     
     6661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     
     6721 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     
     6781 ttcaagaatt gctagcaatt gctagcaatt gctagcaatt cataccagat caccgaaaac     
     6841 tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg ggcttctgcc tcttagacca     
     6901 ctctacccta ttccccacac tcaccggagc caaagccgcg gcccttccgt ttctttgct      
//