pShuttle(+)
| Vector Name | pShuttle(+) |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
541 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
601 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
661 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
721 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
781 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
841 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
901 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
961 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
1021 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
1081 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
1141 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
1201 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
1261 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct
1321 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag
1381 acgccatcca cgctgttttg acctccatag agctagcgtt taaacttaag cttggtaccg
1441 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
1501 gctcgagtct agagggcccg tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc
1561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
1621 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
1681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
1741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag cagatcgatc
1801 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
1861 ttaatgaatc ggccaaggat ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
1921 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
1981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
2041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
2101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
2161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
2281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
2341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
2401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
2461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
2521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
2581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
2641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
2701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
2761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
2821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
2881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
2941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
3001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
3061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
3121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
3181 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
3241 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
3301 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
3361 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
3421 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa
3481 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
3541 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
3601 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
3661 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
3721 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
3781 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
3841 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
3901 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
3961 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
4021 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
4081 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
4141 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
4201 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
4261 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
4321 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat
4381 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca
4441 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
4501 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta
4561 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
4621 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
4681 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca
4741 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
LOCUS dna 4794 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 685..690
/dbxref="REBASE:NruI"
Regulatory_Seq 1299..1338
/gene="tetO reg"
misc_binding 1341..1346
/dbxref="REBASE:AccI"
misc_binding 1428..1433
/dbxref="REBASE:HindIII"
misc_binding 1434..1439
/dbxref="REBASE:KpnI"
misc_binding 1469..1474
/dbxref="REBASE:EcoRI"
misc_binding 1474..1479
/dbxref="REBASE:PstI"
misc_binding 1479..1484
/dbxref="REBASE:EcoRV"
misc_binding 1495..1502
/dbxref="REBASE:NotI"
misc_binding 1502..1507
/dbxref="REBASE:AvrI"
misc_binding 1502..1507
/dbxref="REBASE:AvaI"
misc_binding 1502..1507
/dbxref="REBASE:XhoI"
misc_binding 1508..1513
/dbxref="REBASE:XbaI"
misc_binding 1514..1519
/dbxref="REBASE:ApaI"
misc_binding 1794..1799
/dbxref="REBASE:ClaI"
Rep_Origin 2106..2725
/gene="pBR322 origin"
Promoter 3378..3427
/gene="NEOKAN prom"
ORF 3516..4310
/sequence="ORF_1 rf(3)"
Marker 3519..4307
/gene="NTP_II marker"
Rep_Origin 4415..4720
/gene="f1 origin"
BASE COUNT 1169 a 1161 c 1312 g 1152 t 0 others