pShuttle(-)
| Vector Name | pShuttle(-) |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagcgtttaa acgggccctc
LOCUS dna 4801 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
promoter 651..1392
/gene="CMV"
misc_binding 691..696
/dbxref="REBASE:NruI"
misc_binding 968..973
/dbxref="REBASE:NdeI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1433..1438
/dbxref="REBASE:ApaI"
misc_binding 1439..1444
/dbxref="REBASE:XbaI"
misc_binding 1445..1450
/dbxref="REBASE:AvrI"
misc_binding 1445..1450
/dbxref="REBASE:XhoI"
misc_binding 1445..1450
/dbxref="REBASE:AvaI"
misc_binding 1450..1457
/dbxref="REBASE:NotI"
misc_binding 1468..1473
/dbxref="REBASE:EcoRV"
misc_binding 1473..1478
/dbxref="REBASE:PstI"
misc_binding 1478..1483
/dbxref="REBASE:EcoRI"
misc_binding 1513..1518
/dbxref="REBASE:KpnI"
misc_binding 1519..1524
/dbxref="REBASE:HindIII"
misc_binding 1801..1806
/dbxref="REBASE:ClaI"
Rep_Origin 2113..2732
/gene="pBR322 origin"
Promoter 3385..3434
/gene="NEOKAN prom"
ORF 3523..4317
/sequence="ORF_1 rf(1)"
Marker 3526..4314
/gene="NTP_II marker"
selectable_marker531..4323
/gene="Kan"
Rep_Origin 4422..4727
/gene="f1 origin"
BASE COUNT 1173 a 1167 c 1310 g 1151 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagcgtttaa acgggccctc
1441 tagactcgag cggccgccac tgtgctggat atctgcagaa ttccaccaca ctggactagt
1501 ggatccgagc tcggtaccaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
1561 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
1621 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
1681 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
1741 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcag
1801 atcgatctgc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
1861 atgggtctgc attaatgaat cggccaacgg atcccggtgt gaaataccgc acagatgcgt
1921 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
1981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
2041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
2101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
2161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
2221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
2281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
2341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
2401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
2461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
2581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
2641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
2761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
2821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
2881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
2941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
3001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
3061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
3121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
3181 gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg
3241 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
3301 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
3361 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
3421 gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg
3481 ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
3541 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
3601 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
3661 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
3721 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
3781 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
3841 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
3901 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
3961 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
4021 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
4081 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
4141 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
4201 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
4261 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
4321 ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat
4381 cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa
4441 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
4501 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa
4561 agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
4621 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
4681 tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc
4741 gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct
4801 t