pShuttle-2A-RFP
| Vector Name | pShuttle-2A-RFP |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Additional Information | No Additional Information |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagccttaag aagcttggta
1441 ccgaattcga tatcctgcag agcggccgct ctagcttaga gggcagagga agtcttctaa
1501 catgcggtga cgtggaggag aatcccggcc cttctagaat gagcgagctg atcaaggaga
1561 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
1621 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
1681 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
1741 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
1801 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
1861 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
1921 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
1981 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
2041 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
2101 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
2161 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
2221 tggggcacag agggtctaga tgactcgagt acccatacga cgtcccagac tacgcttgag
2281 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg
2341 ggcgcgatcg ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
2401 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
2461 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
2521 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca
2581 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
2641 ttaatgaatc ggccaacgga tcccggtgtg aaataccgca cagatgcgta aggagaaaat
2701 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
2821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
3001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
3061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
3181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3301 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
3361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
3421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
3841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
3901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3961 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg
4021 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
4081 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
4141 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
4201 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc
4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
4441 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
4801 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt
5101 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat
5161 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat
5221 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
5281 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc
5341 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
5401 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
5461 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg
5521 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
LOCUS dna 5570 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
misc_binding 968..973
/dbxref="REBASE:NdeI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1431..1436
/dbxref="REBASE:HindIII"
misc_binding 1437..1442
/dbxref="REBASE:KpnI"
misc_binding 1443..1448
/dbxref="REBASE:EcoRI"
misc_binding 1449..1454
/dbxref="REBASE:EcoRV"
misc_binding 1455..1460
/dbxref="REBASE:PstI"
misc_binding 1462..1469
/dbxref="REBASE:NotI"
ORF 1539..2243
/sequence="ORF_2 rf(3)"
misc_binding 1995..2007
/dbxref="REBASE:SfiI"
misc_binding 2244..2249
/dbxref="REBASE:XhoI"
misc_binding 2337..2342
/dbxref="REBASE:XmaI"
misc_binding 2337..2342
/dbxref="REBASE:SmaI"
misc_binding 2570..2575
/dbxref="REBASE:ClaI"
misc_binding 2658..2663
/dbxref="REBASE:BamHI"
Rep_Origin 2882..3501
/gene="pBR322 origin"
Promoter 4154..4203
/gene="NEOKAN prom"
ORF 4292..5086
/sequence="ORF_1 rf(2)"
Marker 4295..5083
/gene="NTP_II marker"
Rep_Origin 5191..5496
/gene="f1 origin"