pShuttle-HA

                            LOCUS       dna                      4791 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    691..696
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1305..1344
                     /gene="tetO reg"
     misc_binding    1430..1435
                     /dbxref="REBASE:HindIII"
     misc_binding    1436..1441
                     /dbxref="REBASE:KpnI"
     misc_binding    1442..1447
                     /dbxref="REBASE:EcoRI"
     misc_binding    1448..1453
                     /dbxref="REBASE:EcoRV"
     misc_binding    1454..1459
                     /dbxref="REBASE:PstI"
     misc_binding    1461..1468
                     /dbxref="REBASE:NotI"
     misc_binding    1469..1474
                     /dbxref="REBASE:XbaI"
     misc_binding    1475..1480
                     /dbxref="REBASE:ApaI"
     misc_binding    1481..1486
                     /dbxref="REBASE:XhoI"
     misc_binding    1481..1486
                     /dbxref="REBASE:AvrI"
     misc_binding    1481..1486
                     /dbxref="REBASE:AvaI"
     misc_binding    1791..1796
                     /dbxref="REBASE:ClaI"
     misc_binding    1874..1879
                     /dbxref="REBASE:BamHI"
     Rep_Origin      2103..2722
                     /gene="pBR322 origin"
     Promoter        3375..3424
                     /gene="NEOKAN prom"
     ORF             3513..4307
                     /sequence="ORF_1 rf(3)"
     Marker          3516..4304
                     /gene="NTP_II marker"
     Rep_Origin      4412..4717
                     /gene="f1 origin"
BASE COUNT    1171 a   1164 c   1307 g   1149 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc     
      541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta     
      601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa     
      661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg     
      721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc     
      781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa     
      841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac     
      901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca     
      961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg     
     1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt     
     1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg     
     1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg     
     1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat     
     1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag     
     1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc     
     1381 ctggagacgc catccacgct gttttgacct ccatagagct agccttaaga agcttggtac     
     1441 cgaattcgat atcctgcaga gcggccgctc tagagggccc ctcgagtacc catacgacgt     
     1501 cccagactac gcttgagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag     
     1561 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact     
     1621 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt     
     1681 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat     
     1741 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcag atcgatctct     
     1801 atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta     
     1861 atgaatcggc caaggatcca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa     
     1921 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg     
     1981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg     
     2041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag     
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga     
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc     
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg     
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc     
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca     
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag     
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct     
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc     
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     
     2761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     
     2821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     
     3001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag     
     3061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     
     3181 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt     
     3241 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa     
     3301 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat     
     3361 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct     
     3421 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct     
     3481 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag     
     3541 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg     
     3601 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca     
     3661 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc     
     3721 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg     
     3781 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg     
     3841 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta     
     3901 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag     
     3961 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac     
     4021 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg     
     4081 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg     
     4141 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg     
     4201 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg     
     4261 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat     
     4321 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa     
     4381 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta     
     4441 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca     
     4501 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat     
     4561 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg     
     4621 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc     
     4681 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc     
     4741 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t