pShuttle-His

                            LOCUS       dna                      4782 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    691..696
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1305..1344
                     /gene="tetO reg"
     misc_binding    1430..1435
                     /dbxref="REBASE:HindIII"
     misc_binding    1436..1441
                     /dbxref="REBASE:KpnI"
     misc_binding    1442..1447
                     /dbxref="REBASE:EcoRI"
     misc_binding    1448..1453
                     /dbxref="REBASE:EcoRV"
     misc_binding    1454..1459
                     /dbxref="REBASE:PstI"
     misc_binding    1461..1468
                     /dbxref="REBASE:NotI"
     misc_binding    1469..1474
                     /dbxref="REBASE:XbaI"
     misc_binding    1475..1480
                     /dbxref="REBASE:ApaI"
     misc_binding    1481..1486
                     /dbxref="REBASE:AvaI"
     misc_binding    1481..1486
                     /dbxref="REBASE:XhoI"
     misc_binding    1481..1486
                     /dbxref="REBASE:AvrI"
     Tag             1487..1504
                     /gene="6xHis tag"
     misc_binding    1782..1787
                     /dbxref="REBASE:ClaI"
     misc_binding    1865..1870
                     /dbxref="REBASE:BamHI"
     Rep_Origin      2094..2713
                     /gene="pBR322 origin"
     Promoter        3366..3415
                     /gene="NEOKAN prom"
     ORF             3504..4298
                     /sequence="ORF_1 rf(3)"
     Marker          3507..4295
                     /gene="NTP_II marker"
     Rep_Origin      4403..4708
                     /gene="f1 origin"
BASE COUNT    1170 a   1161 c   1303 g   1148 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc     
      541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta     
      601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa     
      661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg     
      721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc     
      781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa     
      841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac     
      901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca     
      961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg     
     1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt     
     1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg     
     1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg     
     1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat     
     1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag     
     1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc     
     1381 ctggagacgc catccacgct gttttgacct ccatagagct agccttaaga agcttggtac     
     1441 cgaattcgat atcctgcaga gcggccgctc tagagggccc ctcgagcatc atcaccatca     
     1501 ccattgagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt     
     1561 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     
     1621 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     
     1681 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat     
     1741 gcggtgggct ctatggcttc tgaggcggaa agaaccagca gatcgatctc tatgtcgggt     
     1801 gcggagaaag aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg     
     1861 ccaaggatcc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc     
     1921 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     
     1981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     
     2041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     
     2101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     
     2161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     
     2221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     
     2281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     
     2341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     
     2401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     
     2461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     
     2521 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     
     2581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     
     2641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     
     2701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     
     2761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     
     2821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     
     2881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     
     2941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     
     3001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     
     3061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     
     3121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa     
     3181 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc     
     3241 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc     
     3301 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa     
     3361 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa     
     3421 actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag     
     3481 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg     
     3541 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg     
     3601 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc     
     3661 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga     
     3721 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc     
     3781 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag     
     3841 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat     
     3901 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg     
     3961 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca     
     4021 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct     
     4081 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg     
     4141 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg     
     4201 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc     
     4261 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa     
     4321 atcagctcat tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa     
     4381 tagaccgcga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac     
     4441 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa     
     4501 ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct     
     4561 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa     
     4621 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc     
     4681 gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag     
     4741 gatcgaatta attcttaatt aacatcatca ataatatacc tt                        
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