pShuttle-His-18x

                            LOCUS       dna                      4865 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    691..696
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1305..1344
                     /gene="tetO reg"
     misc_binding    1434..1439
                     /dbxref="REBASE:HindIII"
     misc_binding    1440..1445
                     /dbxref="REBASE:KpnI"
     misc_binding    1475..1480
                     /dbxref="REBASE:EcoRI"
     misc_binding    1480..1485
                     /dbxref="REBASE:PstI"
     misc_binding    1485..1490
                     /dbxref="REBASE:EcoRV"
     misc_binding    1503..1510
                     /dbxref="REBASE:NotI"
     misc_binding    1579..1584
                     /dbxref="REBASE:XbaI"
     misc_binding    1585..1590
                     /dbxref="REBASE:ApaI"
     misc_binding    1865..1870
                     /dbxref="REBASE:ClaI"
     Rep_Origin      2177..2796
                     /gene="pBR322 origin"
     Promoter        3449..3498
                     /gene="NEOKAN prom"
     ORF             3587..4381
                     /sequence="ORF_1 rf(2)"
     Marker          3590..4378
                     /gene="NTP_II marker"
     Rep_Origin      4486..4791
                     /gene="f1 origin"
BASE COUNT    1191 a   1193 c   1320 g   1161 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc     
      541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta     
      601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa     
      661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg     
      721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc     
      781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa     
      841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac     
      901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca     
      961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg     
     1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt     
     1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg     
     1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg     
     1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat     
     1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag     
     1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc     
     1381 ctggagacgc catccacgct gttttgacct ccatagagct agcgtttaaa cttaagcttg     
     1441 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg     
     1501 gcgcggccgc aggcggcggc caccatcatc accatcacca ccaccatcac catcaccacc     
     1561 atcaccacca tcactgaatc tagagggccc gtttaaaccg ctgatcagcc tcgactgtgc     
     1621 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     
     1681 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     
     1741 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     
     1801 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca     
     1861 gcagatcgat ctctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt     
     1921 atgggtctgc attaatgaat cggccaagga tccatatgcg gtgtgaaata ccgcacagat     
     1981 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc     
     2041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     
     2101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     
     2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     
     2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     
     2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     
     2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     
     2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     
     2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     
     2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     
     2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     
     3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     
     3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     
     3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     
     3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     
     3241 gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag     
     3301 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga     
     3361 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga     
     3421 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa     
     3481 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg     
     3541 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga     
     3601 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc     
     3661 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc     
     3721 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc     
     3781 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac     
     3841 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc     
     3901 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac     
     3961 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg     
     4021 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct     
     4081 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt     
     4141 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg     
     4201 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac     
     4261 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg     
     4321 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg     
     4381 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca     
     4441 acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga     
     4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc     
     4561 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc     
     4621 gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc     
     4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg     
     4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca     
     4801 gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat     
     4861 acctt                                                                 
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