piLenti-siRNA-GFP

                            
LOCUS       Exported File           8603 bp ds-DNA    circular SYN 04-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piLenti-siRNA-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   .
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             668..848
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    895..1020
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1510..1743
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2408..2648
                     /note="U6 Promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(2725..2939)
                     /note="H1 Promoter"
                     /note="color: #99ccff; direction: LEFT"
     enhancer        2995..3300
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        3301..3504
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3559..4275
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3559 .. 3561 / #05fd14
                        2: 3562 .. 3564 / #05fd14 / 1a
                        3: 3565 .. 4275 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4357..4959
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4972..5559
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5443..5454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 5442"
                     /translation="IEGR"
     misc_feature    5569..5802
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             5622..5802
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5874..5995
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(6324..6912)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             7324..8118
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa
      361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa
      421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
      481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag
     1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga
     1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca
     2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa
     2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg
     2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     2641 gaaaggacaa aaatagtctt ctctagagat atcatttaaa tgtcgacgaa ttcctcgaga
     2701 gaagacagct ttttttggtg tttcgtggtc tcatacagaa cttataagat tcccaaatcc
     2761 aaagacattt cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag
     2821 ggcgccactc ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt
     2881 tcccgcctag tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttct
     2941 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     3001 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     3061 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     3121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     3181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     3241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     3301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     3361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     3421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     3481 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag
     3541 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3601 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3661 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3721 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3781 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3841 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3901 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3961 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4021 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4081 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4141 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     4201 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4261 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg
     4321 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg
     4381 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     4441 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     4501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4621 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4801 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac
     4861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4921 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct
     4981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5341 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc
     5521 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa
     5581 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5761 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6001 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg
     6061 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg
     6121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6961 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7021 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     7081 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     7141 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     7201 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     7261 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
     7321 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7381 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7441 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7501 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7561 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7621 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7681 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     7741 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     7801 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     7861 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7921 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7981 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8041 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8101 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca
     8161 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8221 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8281 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     8341 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     8401 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     8461 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     8521 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat
     8581 taacatcatc aataatatac ctt
//