Column-Pure RNA Miniprep Kit

Cat. No.
G4002
Unit
50 Preparations
Price
$245.00
Cat. No. G4002
Name Column-Pure RNA Miniprep Kit
Unit 50 Preparations
Description

abm’s Column-Pure RNA Miniprep Kit is a fast and efficient method for the isolation and purification of total RNA from mammalian cells, animal tissues, plant tissues, yeast and bacteria. The silica spin column technology allows for rapid recovery of high quality RNA that is ready for downstream applications such as RT-PCR, cDNA libraries, Northern blotting, mRNA purification, nuclease protection assays, and in vitro translation.

Principle:
The Column-Pure RNA technology isolates total RNA by first lysing and homogenizing samples, followed by the addition of ethanol to optimize binding conditions. RNA then binds to the silica membrane, while contaminants are efficiently washed away. For further purity, an optional on-column DNase treatment removes residual DNA traces before eluting the purified RNA in water.

Key Features:

  • Rapid and simple 20 minute protocol
  • Extract high yield and purity RNA from a variety of organisms
  • Unique gDNA removal without DNaseI enzyme 
  • High binding capacity of up to 100 µg RNA
  • No phenol/chloroform, LiCl or alcohol precipitation required
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G4002
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