Mycoplasma can alter cell growth, gene expression, and experimental results without visible signs of contamination. Detect it in less than two hours with a PCR or qPCR kit that fits your workflow.
Start with your PCR instrument, then choose whether you need an internal control or a gel-free workflow.
A straightforward, cost-effective PCR + gel test for routine cell culture screening.
Choose this when an internal control is important for interpreting negative results.
A sensitive, gel-free option for labs with a compatible real-time PCR instrument.
Search your organism of interest to see which kits are designed to detect it.
See how abm Mycoplasma detection performance has been evaluated through an independent peer-reviewed study and side-by-side competitor testing.
Researchers from the CDC’s Arbovirus Reference Collection compared the abm PCR Mycoplasma Detection Kit with Sigma VenorGem and Lonza MycoAlert, using an ATCC assay as the reference. In this study, the abm kit had the highest sensitivity and specificity, and the repository adopted it as its primary Mycoplasma detection kit.
Review abm’s technical comparison data for the Mycoplasma Pro PCR Detection Kit.
Evidence scope: The peer-reviewed study evaluated the abm PCR Mycoplasma Detection Kit used in that publication in a virus-repository quality-control setting. The G239 comparison PDF contains abm-generated supporting data.
Review every detection kit and detection plus elimination bundle in one place.
| Product | Cat. No. | Product Type | Size | Price |
|---|---|---|---|---|
| Mycoplasma PCR Detection Kit | G238 | Detection kit | 100 reactions | Inquiry |
| Mycoplasma Pro PCR Detection Kit | G239 | Detection kit | 100 reactions | Inquiry |
| Mycoplasma qPCR Detection Kit | G240 | Detection kit | 100 reactions | Inquiry |
| Mycoplasma PCR Detection & Elimination Bundle | G238-G398 | Detection + elimination bundle | Bundle | Inquiry |
| Mycoplasma Pro PCR Detection & Elimination Bundle | G239-G398 | Detection + elimination bundle | Bundle | Inquiry |
| Mycoplasma qPCR Detection & Elimination Bundle | G240-G398 | Detection + elimination bundle | Bundle | Inquiry |
Open the specifications, performance data, bundles, or answers you need.
| Compare | G238 | G239 | G240 |
|---|---|---|---|
| Best suited for | Routine, cost-effective screening | Standard PCR with internal control | Sensitive, gel-free screening |
| Detection method | PCR + gel | PCR + gel | Real-time qPCR |
| Target coverage | 200+ Mycoplasma | 300+ Mycoplasma & Acholeplasma | 400+ Mycoplasma & Acholeplasma |
| Internal control | No | Yes | No |
| Sample preparation | No DNA isolation | No DNA isolation | Dilution only |
| Gel electrophoresis | Required | Required | Not required |
| Instrument | Standard PCR | Standard PCR | Compatible real-time PCR |
| Time to results | <2 hours | <2 hours | <2 hours |
G238 detected approximately 10 to 100,000 Mycoplasma genome copies, with a 450 bp positive band.
The 180 bp internal PCR control helps distinguish a genuine negative result from a failed reaction.
A tenfold dilution series from 107 to 1 copy produced a highly linear standard curve with no amplification in the no-template control.
Standard PCR detection plus Mycoplasma elimination support.
View BundleInternal-control PCR detection plus Mycoplasma elimination support.
View BundleGel-free qPCR detection plus Mycoplasma elimination support.
View Bundle* Special introductory price
Mycoplasma contamination may not cause obvious visual changes, yet it can compromise culture quality and downstream experimental results.
Choose G238 for routine standard PCR screening, G239 when you need an internal PCR control, or G240 for real-time qPCR without gel electrophoresis.
Search the Mycoplasma Kit Finder by species, strain, or identifier.
No DNA isolation is required for G238 or G239. G240 uses dilution-only sample preparation.
G240 uses real-time qPCR and does not require gel electrophoresis.
All three workflows are designed to provide results in less than two hours.
Yes. A peer-reviewed Journal of Virological Methods study compared the abm PCR Mycoplasma Detection Kit with Sigma VenorGem and Lonza MycoAlert against an ATCC reference assay across 744 virus preparations. In that study, the abm kit had the highest sensitivity and specificity. Read the publication.
Match a kit to your PCR workflow, confirm coverage for your species or strain, or ask our team for help.
