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Custom CRISPR Services | Design • Cloning • Packaging

Custom CRISPR sgRNA Vectors & Viruses

abm provides a comprehensive range of custom CRISPR sgRNA vector and virus services to support diverse genome editing applications.

Whether your research requires stable or transient expression, viral or non-viral delivery, or in vitro or in vivo studies, our scientists can generate high-quality custom CRISPR constructs tailored to your experimental needs. Choose sgRNA-only vectors for use with an existing Cas system or an All-in-One construct that combines nuclease and sgRNA expression where available.

Find Your Best-Fit Platform Compare Platforms Browse Service Details

Your Decision Path

Start with the outcome your delivery system needs to support

Choose the priority that most closely matches your experiment. We’ll point you toward a practical starting platform, then you can compare specifications and explore the full service details before submitting a project.

1Choose your delivery priority

2Review the best-fit platform

3Build your project brief

What matters most for this project?

Select one starting point. This recommendation is a guide—not a restriction.

Lentivirus

Stable, durable expression

Prioritize stable integration and broad support across dividing, non-dividing, stem, and primary cells.

Explore Lentivirus →

AAV

Tissue-directed or in vivo delivery

Use serotype selection to align delivery with tissue tropism while keeping the system non-integrating.

Explore AAV →

Adenovirus

High-efficiency, non-integrating delivery

Support broad mammalian cell transduction, high titers, and a larger insert capacity without genomic integration.

Explore Adenovirus →

Non-Viral

Simple, transient plasmid delivery

Use an sgRNA-only or All-in-One plasmid when transient expression and straightforward transfection are priorities.

Explore Non-Viral →

Still deciding? Compare every construct format side by side, or explore each platform’s tables, workflows, and FAQs below.

Platform Comparison

Available custom CRISPR vectors & viruses

Use this matrix to quickly identify which delivery platforms support your nuclease and construct format.

Construct Format	AAV Vector/Virus	Lentivector/Virus	Non-Viral Vector	Adenovirus
sgRNA for spCas9	Available	Available	Available	Available
sgRNA for saCas9	Available	—	—	—
All-in-One with spCas9	—	Available	Available	—
All-in-One with saCas9	Available	—	—	—
All-in-One with FnCas12a (FnCpf1)	—	Available	—	—
All-in-One with AaCas12b	—	Available	—	—
sgRNA for AaCas12b	Available	Available	—	—

Service Details

Explore each delivery platform

Review configurations, pricing, packaging specifications, workflows, and FAQs by platform.

Custom CRISPR sgRNA Lentiviral Vectors & Viruses

Choose an All-in-One system or an sgRNA-only vector that can be paired with a Cas9-Expressing Stable Cell Line or spCas9 Lentivirus for stable CRISPR delivery.

Cloning Services

Service	Vector	Unit	Cat. No.	Price
Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – single target	pLenti-U6-sgRNA-PGK-Neo	1.0 μg	C437	$95.00
Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – set of three targets	pLenti-U6-sgRNA-PGK-Neo	3 × 1.0 μg	C441	$250.00
Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – single target	pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro	1.0 μg	C438	$105.00
Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – set of three targets	pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro	3 × 1.0 μg	C442	$280.00
Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – single target	pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP	1.0 μg	C515	$195.00
Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – set of three targets	pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP	3 × 1.0 μg	C516	$465.00
Custom CRISPR All-in-One sgRNA Lentiviral Vector (with AaCas12b) – set of three targets	pLenti-U6-sgRNAaa-SFFV-AaCas12b-2A-Puro	3 × 1.0 μg	G6102	$465.00
Custom CRISPR sgRNA-only Lentiviral Vector (with AaCas12b) – set of three targets	pLenti-U6-sgRNAaa-PGK-Neo	3 × 1.0 μg	G6104	$345.00

Lentivirus Packaging Services

Service	Unit	Titer	Purity	Cat. No.	Lead Time	Price
Custom CRISPR Lentivirus Packaging – single target	4 × 500 μl	10⁷ IU/ml	Research grade	C445	2–3 weeks	$395.00
Custom CRISPR Lentivirus Packaging – three targets, individually packaged	3 × 2 ml	10⁷ IU/ml	Research grade	C447	2–3 weeks	$940.00
Custom CRISPR Lentivirus Packaging – three targets, pooled packaging	4 × 500 μl	10⁷ IU/ml	Research grade	C448	2–3 weeks	$395.00

For additional titer or volume options, please inquire.

Experimental Workflow

sgRNA vector delivery by transfection or lentiviral transduction

Use the custom sgRNA vector directly for transfection or package it into lentivirus for target-cell transduction, followed by gene knockout confirmation.

Lentivirus FAQs

What promoter(s) do your sgRNA and All-in-One lentivectors have?

sgRNAs are expressed from the U6 promoter and Cas9 is expressed from the SFFV promoter.

What is the approximate time frame to generate CRISPR lentiviral constructs?

The typical lead time is around 2–3 weeks for CRISPR lentivectors and 2–3 weeks for lentiviruses. Please inquire for a more accurate lead time.

How should I store my lentivirus?

Aliquots should be made for the lentivirus and stored at −80°C.

If a high-titer custom lentivirus was ordered, is titration performed after a freeze-thaw or before freeze-down?

Titration is performed after aliquoting and freeze-down, so the titer should be accurate after the customer thaws the finished product for the first time.

Which virus is the lentivirus expression system derived from? Is it HIV?

The lentivirus expression system is derived from Human HIV-1 Virus. It employs third-generation self-inactivating recombinant lentiviral vectors with enhanced biosafety features and minimal relation to wild-type Human HIV-1 Virus.

How do you verify the titer?

abm uses the LV900 series qPCR Lentivirus Titration Kit, which quantifies a proprietary region of the lentiviral 5′-LTR.

What is the packaging capacity for lentivirus?

The maximum insert size is <9 kb between the 5′ LTR and 3′ LTR.

Custom CRISPR sgRNA AAV Vectors & Viruses

Choose sgRNA-only or saCas9 All-in-One systems across AAV1–11 and specialty serotypes including AAV2/8, AAV2/9n, AAV2.7m8, PHP.eB, PHP.S, shH10, and Anc80L65. sgRNA-only systems can be paired with a Cas9-Expressing Stable Cell Line, spCas9 Nuclease, or saCas9 Nuclease.

Cloning Services

Service	Vector	Unit	Cat. No.	Price
Custom CRISPR sgRNA AAV vector (for spCas9) – single target	pAAV-U6-sgRNAsp-GFP	1.0 μg	C303	Inquiry
Custom CRISPR sgRNA AAV vector (for spCas9) – set of three targets	pAAV-U6-sgRNAsp-GFP	3 × 1.0 μg	C302	Inquiry
Custom CRISPR sgRNA AAV vector (for saCas9) – single target	pAAV-U6-sgRNAsa-GFP	1.0 μg	C305	Inquiry
Custom CRISPR sgRNA AAV vector (for saCas9) – set of three targets	pAAV-U6-sgRNAsa-GFP	3 × 1.0 μg	C304	Inquiry
Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – single target	pAAV-PGK-saCas9-U6-sgRNAsa	1.0 μg	C307	Inquiry
Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – set of three targets	pAAV-PGK-saCas9-U6-sgRNAsa	3 × 1.0 μg	C306	Inquiry
Custom CRISPR sgRNA AAV vector (for AaCas12b) – set of three targets	pAAV-PGK-GFP-U6-sgRNAaa	3 × 1.0 μg	G6103	Inquiry

AAV Packaging Services

Scale	Application	Purification	Typical Titer	Volume	Price
Low Titer	Cell culture	Supernatant	>1 × 10¹² GC/ml	5 × 100 μl	Choose serotype
High Titer	Cell culture	Gradient	>5 × 10¹² GC/ml	5 × 200 μl	Choose serotype
Ultra-Pure	In vivo	Ultracentrifuge	>1 × 10¹³ GC/ml	10 × 50 μl	Choose serotype

Typical titer is achieved in over 70% of projects. Lower titers may occur with certain AAV serotypes (3, 4, and 6), oversized vectors (>4.7 kb), toxic or unstable sequences, or customer-supplied plasmids.
AAV serotypes 3, 4, and 6 typically produce lower yields; only 50% of the minimum titers listed above can be guaranteed.

Experimental Workflow

CRISPR gene editing by AAV

Choose sgRNA-only AAV for use with Cas9-expressing cells or an All-in-One saCas9 AAV for transduction of cultivated cells or animal models.

AAV Serotype Selection Chart

Review the original serotype selection chart for tissue-tropism guidance. Additional engineered serotypes are available by inquiry.

AAV FAQs

Are your CRISPR sgRNA AAV replication deficient?

Yes. Replication and capsid genes are provided in trans during production, so the packaged virion contains only the ITR sequences and sgRNA; All-in-One AAV also contains saCas9. The cis plasmid and rep/cap plasmid do not share regions of homology, preventing wild-type AAV production through recombination.

What is the packaging capacity for AAV?

The maximum insert size is <4.4 kb.

Is AAV stable? What is the recommended storage temperature?

Store AAV at −80°C for long-term storage. For short-term storage, AAV is stable at 4°C for up to three weeks without significant loss of activity.

Custom CRISPR sgRNA Adenovirus

Custom sgRNA design, cloning, and packaging are delivered in a replication-incompetent (−E1/−E3) human adenovirus type 5 (Ad5) system for knockout, knock-in, activation, and repression workflows.

Adenovirus Packaging Service

Service	Unit	Titer	Cat. No.	Price
Custom CRISPR sgRNA Adenovirus (for spCas9) – single target	1 ml	10⁶ pfu/ml	C446	$660.00

Add-On Services

Service	Unit	Titer	Cat. No.	Price
High Titer Adenovirus Amplification	2 × 1 ml	10¹⁰ pfu/ml	C071	$295.00
Ultra-High Titer Adenovirus Amplification	5 × 200 μl	10¹² pfu/ml	C005	$1250.00
TCID₅₀ Assay using 293 cells	1 Service	—	C008	$350.00

By default, viral titer is calculated by end-point dilution assay. abm cannot guarantee resolution of titer discrepancies involving other quantification methods.
The CRISPR vector backbone used is pAdeno-sgRNA.
abm adenoviruses are replication-incompetent (−E1/−E3) and based on human adenovirus type 5 (Ad5).

Experimental Workflow

CRISPR sgRNA adenovirus workflow

Co-transduce target cells with sgRNA adenovirus and Cas9 adenovirus, then evaluate the resulting change in target gene expression.

Adenovirus FAQs

Does amplification and purification include additional titer verification such as plaque, RCA, or TCID50 assays?

Viral titer is calculated by end-point dilution assay. Additional assays can be provided on request at an additional cost.

Do you sell the adenoviral vectors used to make prepackaged adenovirus?

Adenoviral vectors are currently available only as packaged viral particles. Custom cloning services are available when further modifications are required.

What is the final composition of high-titer, non-purified adenovirus?

High-titer adenoviruses are resuspended in 50 mM Tris-HCl (pH 7.0) with 5% glycerol.

Custom CRISPR sgRNA Non-Viral Vectors

Choose sgRNA-only or spCas9 All-in-One plasmids for transient CRISPR expression. sgRNA-only vectors can be paired with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease, while GFP enables monitoring as the vector is diluted through passaging.

Cloning Services

Service	Vector	Unit	Cat. No.	Price
Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – single target	pNV-sgRNA-GFP	1.0 μg	C439	$135.00
Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – set of three targets	pNV-sgRNA-GFP	3 × 1.0 μg	C443	$360.00
Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – single target	pNV-sgRNA-Cas9-2A-GFP	1.0 μg	C440	$125.00
Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – set of three targets	pNV-sgRNA-Cas9-2A-GFP	3 × 1.0 μg	C444	$355.00

Experimental Workflow

CRISPR gene editing by non-viral vectors

Transfect an sgRNA-only vector into Cas9-expressing cells or use an All-in-One sgRNA/Cas9 vector for cultivated cells, then confirm gene knockout.

CRISPR gene editing workflow by non-viral vectors

Next Step

Found a platform that fits your experiment?

Continue to the project brief so our team can review your target, nuclease, delivery format, cell model, and packaging needs.

Tell Us About Your Project

Project Consultation

Turn your platform choice into a project plan

Share the details you already know. Our scientists can help refine the construct and delivery strategy where the project is still taking shape.

Your current starting pointNot selected yet

Target gene, species, and cell or tissue model
Preferred nuclease and sgRNA-only or All-in-One format
Single target or a set of three targets
Vector only or packaged virus
Application, titer, volume, serotype, or purity needs
Desired timeline and downstream readout

Submit your CRISPR project inquiry

Use the Comments/Notes field to include the project details listed here. There is no commitment required to inquire.

Additional Resources

CRISPR guidance and supporting documents

Explore an introductory CRISPR video and consolidated documentation from the original service pages.

CRISPR Cas9 — A Brief Introduction

Documents

PDF
CRISPR Knockout HandbookGeneral CRISPR guide
PDF
Viral Vector BrochureLentivirus, AAV, and adenovirus
PDF
Enhanced Lentivirus Safety FeaturesReplication incompetency
PDF
Enhanced Adenovirus Safety FeaturesReplication incompetency
PDF
Selection-Drug Killing CurveSupporting selection guidance

Citations

Selected Publications

Fleck JS, et al. “Inferring and perturbing cell fate regulomes in human brain organoids.” Nature (2022). doi: 10.1038/s41586-022-05279-8

Das A, et al. “Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus.” Nature Microbiology (2020). doi: 10.1038/s41564-020-0727-8

Jiang G, et al. “Isorhapontigenin (ISO) inhibits invasive bladder cancer formation in vivo and human bladder cancer invasion in vitro by targeting the STAT1/FOXO1 axis.” Cancer Prevention Research (2016). doi: 10.1158/1940-6207.CAPR-15-0338

Quarato G, et al. “Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis.” Science Advances (2023) 9(21). doi: 10.1126/sciadv.adg8156