Adenovirus
abm offers an affordable, comprehensive collection of premade adenoviruses containing full-length ORFs for human, mouse, and rat genes for gene over-expression studies. Our standard Adenovirus Collection constructs contain the strong CMV promoter with the option of HA or His tags. Can't find the adenovirus you're looking for? Contact our team for a custom construct.
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Advantages of the Adenovirus System:
- High transduction efficiency in most mammalian cells
- Low immunogenicity for high post-infection cell viability
- Non-integrating and no risk of disrupting host genes
- Broad host range (dividing, non-dividing, stem cells, and primary cells)
- High titers can be obtained
- Large insert capacity (up to 6 kb)
- A popular choice in vivo and in vitro applications
- Biosafety: abm use replication-incompetent (-E1/-E3) human adenovirus type 5 (Ad5).
Documents
Top Publications
| 01 | Acetate controls endothelial-to-mesenchymal transition. Zhu X. et al. Cell Metabolism (2023) doi: 10.1016/j.cmet.2023.05.010 |
| 02 | Activation of p38α stress-activated protein kinase drives the formation of the pre-metastatic niche in the lungs. Gui J. et al. Nature Cancer (2020) doi: 10.1038/s43018-020-0064-0 |
| 03 | Generation of orthotopically functional salivary gland from embryonic stem cells. Tanaka J. et al. Nature Communications (2018) doi: 10.1038/s41467-018-06469-7 |
FAQs
| For in vitro use (cell culture studies), is viral purification required? |
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No. If viruses will be used in in vitro cell cultures, double CsCl purification is not required. For in vivo studies (i.e. animal studies), purification is essential in order to remove defective particles, cell debris, and small amounts of media components, since these contaminants induce significant immune responses. In addition, CsCl purification will concentrate the virus to a level suitable for in vivo injections.
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| What are the recommended storage conditions of recombinant Adenoviruses? |
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For long-term storage, the virus should be kept at -80°C, especially after CsCl or chromatography purification. At -80°C in 50 mM Tris (pH 7.0) plus 5% glycerol, the viruses could be stable for several years. Alternatively, viral stock could be stored in 10mM Tris (pH 8.0) plus 4% sucrose for in vivo injection as it is more difficult to do in vivo injection with glycerol formulation.
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