Ready-to-use sgRNA library
Use a pre-designed sgRNA construct from a genome-wide human, mouse, or rat library for a well-characterized knockout target.
Best for: single-gene knockoutWhat is a Cas9-expressing stable cell line?
A Cas9-expressing stable cell line is engineered so that every cell constitutively produces the Cas9 nuclease. Cas9 is stably integrated into the genome, typically through lentiviral or retroviral delivery, rather than transiently transfected. Its expression therefore persists across passages without needing to be reintroduced.
In a standard CRISPR experiment, you deliver the Cas9 nuclease and an sgRNA that tells Cas9 where to cut. With a Cas9-expressing line, the nuclease is already present. Every experiment starts from the same edit-ready background, and only the sgRNA changes.
A consistent, reusable editing background
Three ways to add sgRNA
Choose the delivery route that best matches the target, species, and downstream workflow.
Custom sgRNA vector or virus
Design and package a custom sgRNA for a target outside the standard library, a non-standard species, or a cell-specific delivery format.
Best for: novel or non-human targetsTransfected in vitro-transcribed sgRNA
Transfect synthetic sgRNA directly for transient, plasmid-free editing when avoiding integrating vectors matters downstream.
Best for: transient, footprint-free editingPlanning a knock-in? The same three routes apply, with one addition: deliver a homology-directed repair (HDR) template alongside the sgRNA to introduce the desired sequence at the cut site. Explore custom CRISPR knock-in repair templates.
Search and filter Cas9-expressing cell lines
Browse the full legacy catalogue by product name, catalogue number, species, or tissue. Filters update instantly and can be combined.
67 cell lines found
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Ordering notes: Sale is subject to completion of a Material Transfer Agreement (MTA) by the purchasing institution. For-profit organizations should contact quotes@abmgood.com for pricing. Cell Line Insurance is also available for added protection against growth, thawing, or storage-loss issues.
Cas9-expressing line vs. ready-to-use CRISPR KO line
| Consideration | Cas9-expressing cell line | Ready-to-use CRISPR KO cell line |
|---|---|---|
| Gene status | Intact — Cas9 is present, but the target gene is not yet edited | Already knocked out and validated |
| What you still need | An sgRNA, plus a repair template for knock-in | Nothing — ready to use on arrival |
| Best for | Screens, custom or multiple targets, knock-ins, and ongoing editing programs | A single, specific, well-defined gene knockout |
| Flexibility | One line supports edits at any locus, at any time | Fixed to the gene already knocked out |
Need a finished knockout instead? Explore ready-to-use CRISPR KO cell lines.
Cas9-expressing stable cell line FAQ
What is a Cas9-expressing stable cell line?
A cell line engineered to permanently express the Cas9 nuclease in every cell, generation after generation. Because Cas9 is already built in, you only need to introduce a guide RNA (sgRNA) to perform a CRISPR genome-editing experiment rather than delivering Cas9 and sgRNA together each time.
How is a Cas9-expressing cell line different from a CRISPR knockout (KO) cell line?
A Cas9-expressing line is a starting material: Cas9 is present, but your gene of interest remains intact until you add an sgRNA. A CRISPR KO line is a finished product in which the target gene has already been disrupted and validated. Choose a Cas9-expressing line for screens, custom targets, multiple targets, or knock-ins; choose a ready-made KO line for one validated gene knockout.
Do I still need to design my own sgRNA?
Yes. Cas9-expressing lines provide the nuclease only. You will still need an sgRNA targeting your gene, sourced from a ready-to-use genome-wide library, a custom sgRNA vector or virus, or transfected in vitro-transcribed sgRNA.
Can these cell lines be used for CRISPR knock-in?
Yes. Because Cas9 is already stably expressed, knock-in requires an sgRNA plus a homology-directed repair (HDR) template. You are not also delivering Cas9, which reduces payload size and can be especially helpful for larger insertions.
What species and cell types are available?
The supplied catalogue includes human, mouse, rat, chicken, Chinese hamster, dog, and green monkey lines spanning kidney, liver, lung, brain, blood and immune, cardiac, colon, ovary, skin, pancreas, and other tissues.
How is Cas9 expression confirmed in these cell lines?
Cas9 expression is confirmed by Western blot with cell-line-specific quality-control data. Editing competency can then be verified after sgRNA introduction using assays such as the Surveyor assay or targeted sequencing.
Are negative control cell lines available?
Yes. Scrambled Cas9-expressing negative control lines, including TeloHAEC and MPNST JW23.3, are available for isogenic comparisons within the same cellular background.
Can these lines be used for pooled or arrayed CRISPR screens?
Yes. Consistent Cas9 expression across the population makes these lines well suited to pooled and arrayed sgRNA library screens, a standard architecture for functional genomics screening.
What if my cell type is not in the catalogue?
abm's Custom Stable Cell Line Generation Service can engineer Cas9 expression into a cell line of your choice, including proprietary lines, with a defined turnaround time and a full quality-control data package.
Is a Material Transfer Agreement (MTA) required?
Yes. Sale of Cas9-expressing cell lines is subject to completion of an MTA by the purchasing institution. For-profit organizations should contact abm directly for pricing.
Ready to start editing?
Browse abm's collection of 60+ Cas9-expressing stable cell lines across seven species, complete with cell-line-specific QC and Western blot verification.