Custom CRISPR Multiplex sgRNA Vectors
abm’s Custom CRISPR Multiplex sgRNA system allows for optimal expression of multiple sgRNAs from alternating U6 and H1 RNA pol III promoters on a single lentiviral vector. This allows for expression of multiple sgRNAs targeting a single gene or multiple sgRNAs targeting multiple different genes. sgRNAs may be designed for use with any Cas9 variant, which is expressed from a separate vector. Together, the Multiplex sgRNA Vectors expand the CRISPR toolkit allowing for highly diverse gene editing applications. Inquire with us below to get started on your custom CRISPR project.
Applications
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- Gene Activation using dCas9-VPR
- Gene Repression using dCas9-KRAB
- Epigenetic Gene Activation using dCas9-TET1CD
- Epigenetic Gene Repression using dCas9-DNMT3A
- Gene Knockout using spCas9
Service Details
Cloning Services
| sgRNA Service (for spCas9) | No. of sgRNAs | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Dual sgRNA Lentivector (for spCas9) | 2 | 1.0 µg | C420 | $395.00 |
| Custom CRISPR Triple sgRNA Lentivector (for spCas9) | 3 | 1.0 µg | C421 | $595.00 |
| Custom CRISPR Quad sgRNA Lentivector (for spCas9) | 4 | 1.0 µg | C422 | $795.00 |
| Custom CRISPR Multiplex sgRNA Lentivector (for spCas9) | >4 | 1.0 µg | C423 | Inquire |
| sgRNA Service (for saCas9) | No. of sgRNAs | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Dual sgRNA Lentivector (for saCas9) | 2 | 1.0 µg | C511 | $395.00 |
| Custom CRISPR Triple sgRNA Lentivector (for saCas9) | 3 | 1.0 µg | C512 | $595.00 |
| Custom CRISPR Quad sgRNA Lentivector (for saCas9) | 4 | 1.0 µg | C513 | $795.00 |
| Custom CRISPR Multiplex sgRNA Lentivector (for saCas9) | >4 | 1.0 µg | C514 | Inquire |
Lentivirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer Custom Recombinant Lentivirus Packaging | 4 x 500 μl | 107 IU/ml | Research grade | LV001-a | 2-3 weeks | $199.00 |
| Medium Titer Custom Recombinant Lentivirus Packaging | 4 x 250 µl | 108 IU/ml | Research grade | LV001-b | 2-3 weeks | $449.00 |
| High Titer Custom Recombinant Lentivirus Packaging | 10 x 100 µl | 109 IU/ml | Research grade | LV001-c | 2-3 weeks | $749.00 |
| Ultra-High Titer Custom Recombinant Lentivirus Packaging | 10 x 100 µl | 1010 IU/ml | Research grade | LV001-d | 2-3 weeks | $1049.00 |
| Custom Lentivirus Aliquoting | Up to 10 Vials | - | - | C507 | - | $50.00 |
Additional Info
Additional Resources
Documents
FAQs
| What promoter(s) are used in the Multiplex sgRNA vectors? |
|
sgRNAs are expressed from alternating U6 and H1 promoters.
|
| What is the approximate time frame to generate CRISPR lentiviral constructs? |
|
The typical lead time is around 2-3 weeks for CRISPR lentivectors and 2-3 weeks for lentiviruses. Please inquire for a more accurate lead time.
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Citations
| 01 | Metzger D. et al. "Host Cell Transcriptional Tuning with CRISPR/dCas9 to Mitigate the Effects of Toxin Exposure." ACS Synth. Biol. (2022) 11, 3657-3668. doi: 10.1021/acssynbio.2c00214 |
| 02 | Shirasaki, T. et al. "Nonlytic Quasi-Enveloped Hepatovirus Release Is Facilitated by pX Protein INteraction with the E3 Ubiquitin Ligase ITCH." J. Virol. (2022) 96(21). doi: 10.1128/jvi.01195-22 |