Custom Retrovirus Service
abm’s custom retroviruses are a highly-efficient method to achieve stable gene expression in a broad range of dividing mammalian cells. Simply send us your gene, or purchase an ORF vector from our extensive library of human, mouse, or rat genes, and we will do the rest!
| Key Features | |
|---|---|
| Promoter | CMV promoter for strong gene expression in most cellular systems |
| Tags Available | No tag HA tag for detection or protein purification GFP reporter |
| Safety | Replication-incompetent retrovirus with minimal relation to the wild-type Moloney Murine Leukemia Virus (MoMuLV) |
Service Details
Gene Synthesis and Custom Cloning
| Service | Description | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom Retroviral Vector using ORF Library with No Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with no tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C362 | $150.00 |
| Custom Retroviral Vector using ORF Library with HA Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a C-term HA tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C363 | $300.00 |
| Custom Retroviral Vector using ORF Library with GFP Reporter | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a GFP reporter. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 3.2 kb. |
1.0 μg | C364 | $300.00 |
| Custom Retroviral Vector | Custom retrovirus plasmid subcloning. Provide your DNA and we’ll subclone it into a retroviral vector. | 1 Service | C057 | $550.00 |
| Gene Synthesis* | For any sequence up to 4.5 kb in size. Genes are fully-sequenced. Must be ordered with Custom Retroviral Vector (C362, C363 or C364). * Minimum charge of $99.00 |
1.0 μg | C098 | $0.18/bp USD |
Custom Retrovirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Custom Retrovirus Packaging | 4 x 250 µl | 107 IU/ml | Research-grade | C510 | 3-4 weeks | $600.00 |
Add-on Services
| Service | Cat. No. | Price |
|---|---|---|
| Plasmid Amplification for Virus Packaging Service | C314 | $100.00 |
- Customer must supply >60µg of their retroviral vector at a concentration of 1µg/µl. If the supplied amount of retroviral vector does not meet this criteria an additional Add-On Service (Cat. No. C314) will be applied.
- For customer supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
Available Retroviral Vectors and Controls
Retroviral Vectors
Retrovirus Controls
| Product | Unit | Cat. No. | Price |
|---|---|---|---|
| pRetroG-CMV-blank Vector | 1.0 μg | RV001 | $135.00 |
| pRetroG-CMV-GFP-HA Vector | 1.0 μg | RV002 | $135.00 |
| pRetroG-CMV-GFP-stop Vector | 1.0 μg | RV003 | $135.00 |
| pRetroG-CMV-RFP-stop Vector | 1.0 μg | RV005 | $135.00 |
| Retro-CMV Blank Virus | 1 ml of 107 IU/ml | RVP001 | $195.00 |
| Retro-CMV-GFP-HA Virus | 1 ml of 107 IU/ml | RVP002 | $195.00 |
| Retro-CMV-GFP Virus | 1 ml of 107 IU/ml | RVP003 | $195.00 |
| Retro-CMV-RFP Virus | 1 ml of 107 IU/ml | RVP005 | $195.00 |
Additional Info
FAQs
| How do you determine titer? |
|
We perform qPCR titration.
|
| What is the packaging capacity for Retrovirus? |
|
The maximum insert size is <8 kb between the 5'LTR to 3'LTR.
|
Citations
| 01 | Thomas, A. M. et al. "Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury." J Control Release. 3:421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013 PubMed: 23791981 |