Custom Cloning & Gene Synthesis Service

• For non-complicated sequences
• Synthesize up to 950 bp
• Complimentary subcloning into pUC57, EcoRV site
• Sequence verification to confirm full length sequence is generated
• Sequence must be approved for economy synthesis by abm

• For any sequence up to 4 kbp in size
• Genes are fully sequenced
• Must be ordered with Subcloning Service I (C096)
• Minimum charge of USD$99.00

• Subclone any insert into an expression system of your choice. This service is intended for inserts that are compatible with restriction sites of the expression vector of choice.
• Sequencing of the insert is performed to confirm proper insertion.
• For full sequencing, please refer to C197.

• Subclone any insert into an expression system of your choice. This service is intended for inserts that are not compatible with restriction sites of the abm expression vector of choice, or if cloning involves customer provided plasmids.
• Full length DNA sequencing is performed for sequence verification.
• Genes over 3 kbp are subject to an additional charge of $100.00/kbp (C100).

• Mutate up to 25 bp (consecutive) within a < 2 kbp fragment in any existing abm product or a provided vector.
• Full length sequencing to confirm presence of mutation.
• Fragments over 2 kbp are subject to an additional charge of $100.00/kbp (C100).
• A minimum order of $450 is required to qualify for this service.

• Additional charge for fragments over the stated size limit ($100.00/kbp)
• Only applicable to C097, C047 and C128.

• Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1α, PGK, UbC

• Addition/Removal of an N-terminal or C-terminal tag smaller than 30 bp

• Addition/Removal of an N-terminal or C-terminal tag larger than 30 bp
• Tags over 1 kbp are subject to an additional charge of $100.00/kbp (C100).

• Addition, removal or switching of a reporter (e.g. GFP, RFP, Luc) in one of abm’s vectors or a vector of your choice.

• Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice.

• Usage of an abm vector backbone for a Custom Cloning Service (excludes pORF and pUC57).

Bacterial stab of the custom plasmid.

Research-grade, plasmid amplification.

Research-grade, plasmid amplification.

Amplification of the custom plasmid.

Endotoxin-Free, bulk high copy plasmid amplification. 

Endotoxin-Free, bulk high copy plasmid amplification.

Endotoxin-Free, bulk high copy plasmid amplification.