CRISPR Activation & Repression
abm's CRISPR Activation sgRNA vectors and viruses are highly effective at achieving activation of your target gene. CRISPR Activation (CRISPRa) allows for gene specific up-regulation by using dCas9-VPR. This fusion protein consists of the catalytically dead Cas9 (dCas9) enzyme fused to the tripartite complex VPR (VP64, p65 and Rta). We offer a comprehensive collection of sgRNA-only lentiviral vectors expressing sgRNAs that target the upstream 5' UTR promoter region of any human, mouse or rat gene thereby resulting in transcriptional up-regulation. Can't find the construct you're looking for? Contact our team for Custom sgRNA and Custom multiple sgRNA services.
Search Activation sgRNA Library
Search our collection of CRISPR gene activation sgRNA products using gene name or accession number:
Additional Information
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Vector Map
sgRNA for activation of your gene of interest is available in lentiviral vector or lentivirus format. -
Workflow
How to use abm's activation sgRNA lentiviruses in your CRISPR activation experiment. -
Need Help Getting Started?
Read our CRISPR Activation and Repression Knowledge Base article for more information.
Documents
Top Publications
Inferring and perturbing cell fate regulomes in human brain organoids.
Fleck JS. et al.
Nature (2022)
doi: 10.1038/s41586-022-05279-8
Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus.
Das A. et al.
Nature Microbiology (2020)
doi: 10.1038/s41564-020-0727-8
Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.
Yamane D. et al.
Nature Microbiology (2019)
doi: 10.1038/s41564-019-0425-6