Primer & Probe Design
abm offers a one-stop Custom qPCR Primer and TaqMan Probe Design Service to save time, effort, and money by preventing complications or failures in qPCR experiments. Well-designed primers and probes are crucial for success. With years of expertise, abm scientists use in-house developed software to design qPCR primers with high specificity, minimizing primer dimer formation. Similarly, for TaqMan probes, virtual simulations ensure minimal secondary structure complications at the binding region, promoting effective binding and efficient hydrolysis.
To order, follow these steps:
- Download and fill out the order form.
- Primer Form for primers
- Probe Form for TaqMan probes
- Include the following:
- Primers: Oligo name, sequence, synthesis scale
- Probes: Probe name, sequence, 5’/3’ modifications, synthesis scale
- Email to: quotes@abmgood.com
For TaqMan probes, two designs are provided, with re-design at no charge if both fail.
Service Details
| Service Name | Unit | Cat. No. | Price |
|---|---|---|---|
| Primer Design Service | 1 Pair Design | C130 | $80.00 |
| Primer Synthesis (25 nmol) | 1 Base | C132 | $0.35* |
| Primer Synthesis (100 nmol) | 1 Base | C133 | $0.70* |
| Primer Synthesis (250 nmol) | 1 Base | C231 | $1.25* |
| sgRNA PCR Primer Pair Design & Synthesis Service (25 nmol/primer) | 1 Pair | C336 | $99.00 |
| sgRNA Target-Specific Oligo Design & Synthesis Service (100 nmol) | 1 Oligo | C337 | $120.00 |
| Service Name | Quantity | Cat. No. | Price |
|---|---|---|---|
| TaqMan Probe Design Service | 1 Target Design (2 Probes) | C131 | $250.00 |
| Probe Synthesis (FAM/BHQ1) | 100 nmol | C134 | $325.00 |
| Probe Synthesis (FAM/BHQ1) | 250 nmol | C135 | $387.70 |
| Probe Synthesis (HEX/BHQ1) | 100 nmol | C136 | $348.50 |
| Probe Synthesis (HEX/BHQ1) | 250 nmol | C137 | $492.50 |
| Probe Synthesis (ROX/BHQ2) | 250 nmol | C331 | $590.00 |
| Probe Synthesis (Cy5/BHQ2) | 100 nmol | C332 | $448.00 |
| Probe Synthesis (Cy5/BHQ2) | 250 nmol | C333 | $520.00 |
| Probe Synthesis (FAM/TAMRA) | 100 nmol | C334 | $325.00 |
| Probe Synthesis (FAM/TAMRA) | 250 nmol | C335 | $387.50 |
Additional Info
Standard Desalted Custom DNA Oligo Synthesis
| Cat. No | Synthesis Scale | Minimum Guaranteed Yield | Required Primer Length |
|---|---|---|---|
| C132 | 25 nmol | 10 nmol | 15-60 nt |
| C133 | 100 nmol | 30 nmol | 10-90 nt |
| C231 | 250 nmol | 50 nmol | 5-100 nt |
abm understands primer design can make or break your PCR/qPCR experiment - check out some examples below:
Traditional Dual-labelled Probes
| Cat. No | 5’ Reporter Dye | Quencher | Synthesis Scale | Min Guarantee Yield (OD) | Min Guarantee Yield (nmol) |
|---|---|---|---|---|---|
| C134 | 6’FAM | BHQ1 | 100 nmol | 2.0 OD | 10 nmol |
| C135 | 6’FAM | BHQ1 | 250 nmol | 5.0 OD | 25 nmol |
| C136 | HEX | BHQ1 | 100 nmol | 2.0 OD | 10 nmol |
| C137 | HEX | BHQ1 | 250 nmol | 5.0 OD | 25 nmol |
| C331 | ROX | BHQ2 | 250 nmol | 1.5 OD | 8 nmol |
| C332 | Cy5 | BHQ2 | 100 nmol | 0.4 OD | 2 nmol |
| C333 | Cy5 | BHQ2 | 250 nmol | 1.5 OD | 8 nmol |
| C334 | 6'FAM | TAMRA | 100 nmol | 2.0 OD | 10 nmol |
| C335 | 6'FAM | TAMRA | 250 nmol | 5.0 OD | 25 nmol |
1. The synthesis scale indicates the initial amount of 3'-bases.
2. Average OD yield was determined for a 20-mer; Calculation: 1 OD = 5 nmol = 30 ug; may vary for sequences with GC content >70%, >3 purine stretches, or strong secondary structures.
Customer Review:
"I am a fan of abm: great product quality, great price, great rewards and great experience."
Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL