As a leading expert in AAV technology, abm provides a Custom AAV Service to assist with the design, cloning, and viral packaging of your unique AAV construct. We offer a wide selection of AAV serotypes, including 1-11, AAV2/8, AAV2/9n, AAV2.7m8, PHP.eB, PHP.S, shH10 and Anc80L65. Our purification options include crude preparations (ideal for in vitro experiments) and purified versions (optimized for in vivo applications). Inquire with us below to get started on your Custom AAV project.
Advantages of the AAV System:
Used as a promising candidate for gene therapy
Does not elicit significant immune responses in vivo
Broad tropism - tissue specificity with different AAV serotypes
No integration into the host genome
Ability to transduce both proliferating and quiescent cells
Custom AAV plasmid cloning without compatible insert
1 Service
C235
$450.00
*For these services, the customer must provide a gene insert under 3kb. Please contact technical@abmgood.com for more information.
Custom AAV Packaging Services
Service
Unit
Titer
Purity
Cat. No.
Lead Time
Price
Standard Titer Custom Recombinant AAV Packaging
3 x 100 μl
1011 GC/ml
Standard
2-3 weeks
$325
High Titer Custom Recombinant AAV Packaging
5 x 200 μl
1012 GC/ml
Ultra-pure
3-4 weeks
$895
Ultra-High Titer Custom Recombinant AAV Packaging
10 x 50 μl
1013 GC/ml
Ultra-pure
3-4 weeks
$1695
Add-on Services
Service
Unit
Cat. No.
Price
Custom AAV Aliquoting
Up to 10 Vials
C506
$50
Custom AAV Titration using qPCR Assay
1 Service
C238
$85
Plasmid Amplification for Virus Packaging Service
1 Service
C314
$100
Customer must supply their AAV vector along with plasmid map and full sequence.
Customer must supply their AAV vector at a concentration >0.5µg/ml with the following amounts (1011 GC/ml: 75µg; 1012 GC/ml: 400µg; 1013 GC/ml: 800µg. If the supplied amount of AAV vector does not meet these criteria, an additional Add-on Service (Cat. No. C314) will be applied.
Customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
Service Cat. No. C506 requires the minimum volume aliquoted per vial of 25 µl.
Supporting Data
Left: EGFP expression (Green) in lumbar neuronal cells 4 weeks after intrathecal injection of AAV-EGFP Serotype 9 (Cat. # iAAV01509) into mice. Right: Overlay with β-tubulin (red) and DAPI (blue). Image courtesy of Dr. Douglas Lopes, King’s College London.
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
Zhang, Y. et al. "Hypothalamic stem cells control ageing speed partly through exosomal miRNAs." Nature. 548(7665):52-57 (2017). DOI: 10.1038/nature23282. PubMed: 28746310. Application: In vivo infection.
Papes, F. et al. "Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content." Nature Communications. 13:2387. (2022). DOI: 10.1038/s41467-022-29942-w. PubMed: 35501322.
Li, J. et al. "Activation of the chemokine receptor CCR1 and preferential recruitment of Gαi suppress RSV replication: implications for developing novel RSV treatment strategies." Journal of Virology. 96(22):e0130922. (2022). DOI: 10.1128/jvi.01309-22. PubMed: 36317881
Scano, M. et al. "CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3." Human Molecular Genetics. 31(4):499-509. (2022). DOI: 10.1093/hmg/ddab260. PubMed: 34505136
