Ni-IDA Agarose Resins
Cat. No. | G250 |
Name | Ni-IDA Agarose Resins |
Category | Buffers & General Chemicals |
Description |
Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
Special Features
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Application |
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Storage Condition |
Resin should be stored at 2-8°C. Do not freeze. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G250 |
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mM)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G945\u0022\u003E$99.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/ntp-set-g946-vin.html\u0022 rel=\u0022noopener\u0022\u003ENTP Set\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/ntp-set-g946-vin.html\u0022 rel=\u0022noopener\u0022\u003EG946\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E4 x 250 \u0026micro;l (100 mM each)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G946\u0022\u003E$109.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left\u0022 class=\u0022abm-page-menu\u0022 id=\u0022table-product-mini-category-BuffersChemicals\u0022\u003E\n\u003Ctd colspan=\u00224\u0022\u003E\u003Cspan style=\u0022color: #000;\u0022\u003E\u003Cstrong\u003EBuffers \u0026amp; Chemicals\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-pbs-solution-ch111.html\u0022 rel=\u0022noopener\u0022\u003E1X PBS, pH 7.2\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-pbs-solution-ch111.html\u0022 rel=\u0022noopener\u0022\u003ECH111\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH111\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-pbs-solution-ch110.html\u0022 rel=\u0022noopener\u0022\u003E1X PBS, pH 7.4\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-pbs-solution-ch110.html\u0022 rel=\u0022noopener\u0022\u003ECH110\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH110\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-pbs-solution-ch113.html\u0022 rel=\u0022noopener\u0022\u003E10X PBS, pH 7.2\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-pbs-solution-ch113.html\u0022 rel=\u0022noopener\u0022\u003ECH113\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH113\u0022\u003E$60.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-pbs-solution-ch112.html\u0022 rel=\u0022noopener\u0022\u003E10X PBS, pH 7.4\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-pbs-solution-ch112.html\u0022 rel=\u0022noopener\u0022\u003ECH112\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH112\u0022\u003E$60.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-pipes-buffer-solution-ch101.html\u0022 rel=\u0022noopener\u0022\u003E1 M PIPES Buffer Solution, pH 8\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-pipes-buffer-solution-ch101.html\u0022 rel=\u0022noopener\u0022\u003ECH101\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH101\u0022\u003E$205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/tris-acetate-edta-buffer-ch117.html\u0022 rel=\u0022noopener\u0022\u003E50X TAE Buffer (Tris-Acetate-EDTA)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/tris-acetate-edta-buffer-ch117.html\u0022 rel=\u0022noopener\u0022\u003ECH117\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH117\u0022\u003E$50.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/te-buffer-ch114.html\u0022 rel=\u0022noopener\u0022\u003E1X TE Buffer, pH 7\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/te-buffer-ch114.html\u0022 rel=\u0022noopener\u0022\u003ECH114\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH114\u0022\u003E$70.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/te-buffer-ch115.html\u0022 rel=\u0022noopener\u0022\u003E1X TE Buffer, pH 8\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/te-buffer-ch115.html\u0022 rel=\u0022noopener\u0022\u003ECH115\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH115\u0022\u003E$70.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-tris-buffered-saline-solution-ch108.html\u0022 rel=\u0022noopener\u0022\u003E1X Tris Buffered Saline (TBS)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1x-tris-buffered-saline-solution-ch108.html\u0022 rel=\u0022noopener\u0022\u003ECH108\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH108\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-tris-buffered-saline-solution-ch109.html\u0022 rel=\u0022noopener\u0022\u003E10X Tris Buffered Saline (TBS)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-tris-buffered-saline-solution-ch109.html\u0022 rel=\u0022noopener\u0022\u003ECH109\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH109\u0022\u003E$50.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-tris-glycine-sds-buffer.html\u0022 rel=\u0022noopener\u0022\u003E10X Tris-Glycine-SDS Buffer\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/10x-tris-glycine-sds-buffer.html\u0022 rel=\u0022noopener\u0022\u003ECH125\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH125\u0022\u003E$55.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/100mm-tris-hcl-buffer-ch107.html\u0022 rel=\u0022noopener\u0022\u003E100 mM Tris-HCl Buffer, pH 7.4\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/100mm-tris-hcl-buffer-ch107.html\u0022 rel=\u0022noopener\u0022\u003ECH107\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH107\u0022\u003E$28.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch102.html\u0022 rel=\u0022noopener\u0022\u003E1 M Tris-HCl Buffer, pH 7\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch102.html\u0022 rel=\u0022noopener\u0022\u003ECH102\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH102\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch103.html\u0022 rel=\u0022noopener\u0022\u003E1 M Tris-HCl Buffer, pH 7.5\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch103.html\u0022 rel=\u0022noopener\u0022\u003ECH103\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH103\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch104.html\u0022 rel=\u0022noopener\u0022\u003E1 M Tris-HCl Buffer, pH 7.6 \u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch104.html\u0022 rel=\u0022noopener\u0022\u003ECH104\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH104\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch106.html\u0022 rel=\u0022noopener\u0022\u003E1 M Tris-HCl Buffer, pH 8.5\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/1m-tris-hcl-buffer-solution-ch106.html\u0022 rel=\u0022noopener\u0022\u003ECH106\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH106\u0022\u003E$35.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/edta.html\u0022 rel=\u0022noopener\u0022\u003E0.5 M EDTA\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/edta.html\u0022 rel=\u0022noopener\u0022\u003ECH124\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH124\u0022\u003E$105.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/2-mercaptoethanol-ch045.html\u0022 rel=\u0022noopener\u0022\u003E2-Mercaptoethanol\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/2-mercaptoethanol-ch045.html\u0022 rel=\u0022noopener\u0022\u003ECH045\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E200 ml (2 x 100ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH045\u0022\u003E$75.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/magnesium-chloride-ch119.html\u0022 rel=\u0022noopener\u0022\u003E1 M MgCl\u2082 Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/magnesium-chloride-ch119.html\u0022 rel=\u0022noopener\u0022\u003ECH119\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH119\u0022\u003E$40.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/magnesium-sulfate-ch118.html\u0022 rel=\u0022noopener\u0022\u003E1 M MgSO\u2084 Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/magnesium-sulfate-ch118.html\u0022 rel=\u0022noopener\u0022\u003ECH118\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH118\u0022\u003E$15.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/manganese-chloride-solution-ch121.html\u0022 rel=\u0022noopener\u0022\u003E25 mM MnCl\u2082 Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/manganese-chloride-solution-ch121.html\u0022 rel=\u0022noopener\u0022\u003ECH121\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH121\u0022\u003E$40.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/nacl-solution.html\u0022 rel=\u0022noopener\u0022\u003E4 M NaCl Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/nacl-solution.html\u0022 rel=\u0022noopener\u0022\u003ECH120\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH120\u0022\u003E$45.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/peg6000-solution-ch123.html\u0022 rel=\u0022noopener\u0022\u003E50% (v\/w) PEG6000 Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/peg6000-solution-ch123.html\u0022 rel=\u0022noopener\u0022\u003ECH123\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH123\u0022\u003E$445.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/peg8000-solution-ch122.html\u0022 rel=\u0022noopener\u0022\u003E40% (v\/w) PEG8000 Solution\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/peg8000-solution-ch122.html\u0022 rel=\u0022noopener\u0022\u003ECH122\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E500 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH122\u0022\u003E$625.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/sds-sodium-dodecyl-sulfate-solution.html\u0022 rel=\u0022noopener\u0022\u003E20% SDS\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/sds-sodium-dodecyl-sulfate-solution.html\u0022 rel=\u0022noopener\u0022\u003ECH100\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022CH100\u0022\u003E$65.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/3m-sodium-acetate-solution-ch126.html\u0022 rel=\u0022noopener\u0022\u003E3 M Sodium Acetate Solution, pH 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