Custom CRISPR sgRNA AAV Vectors & Viruses
abm provides custom AAV vectors and viruses for CRISPR/Cas9 experiments, offering both All-in-One (Cas9 and sgRNA expressing) and sgRNA-only expression systems. Our extensive selection of AAV serotypes includes 1-11, AAV2/8, AAV2/9n, AAV2.7m8, PHP.eB, PHP.S, shH10, and Anc80L65. Choose from our All-in-One system (featuring the saCas9 variant) or our sgRNA-only expression system and pair the latter with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease or saCas9 Nuclease. Inquire with us below to get started on your custom CRISPR project.
Advantages of the AAV System:
- Used as a promising candidate for gene therapy
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Service Details
Cloning Services
| Service | Vector | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA AAV vector (for spCas9) – single target | pAAV-U6-sgRNAsp-GFP-hGH-amp | 1.0µg | C303 | $145.00 |
| Custom CRISPR sgRNA AAV vector (for spCas9) – set of three targets | pAAV-U6-sgRNAsp-GFP-hGH-amp | 3x1.0µg | C302 | $345.00 |
| Custom CRISPR sgRNA AAV vector (for saCas9) – single target | pAAV-U6-sgRNAsa-GFP-hGH-amp | 1.0µg | C305 | $145.00 |
| Custom CRISPR sgRNA AAV vector (for saCas9) – set of three targets | pAAV-U6-sgRNAsa-GFP-hGH-amp | 3x1.0µg | C304 | $345.00 |
| Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – single target | pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp | 1.0µg | C307 | $195.00 |
| Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – set of three targets | pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp | 3x1.0µg | C306 | $465.00 |
AAV Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer Custom Recombinant AAV Packaging | 3 x 100 μl | 1011 GC/ml | Standard | 2-3 weeks | $325 | |
| High Titer Custom Recombinant AAV Packaging | 5 x 200 µl | 1012 GC/ml | Ultra-pure | 3-4 weeks | $895 | |
| Ultra-High Titer Custom Recombinant AAV Packaging | 10 x 50 μl | 1013 GC/ml | Ultra-pure | 3-4 weeks | $1695 |
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector /Virus |
Lentivector /Virus |
Non-Viral Vector |
Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| Browse Collection | Browse Collection | Browse Collection | Browse Collection |
AAV Serotype Selection Chart
Additional Resources
Documents
FAQs
| Are your CRISPR sgRNA AAV replication deficient? |
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Yes, the replication and capsid genes are provided in trans when the AAV are produced, therefore the packaged virion only has the ITR sequences and the sgRNA (and All-in-One AAV have saCas9). Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
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| What is the packaging capacity for AAV? |
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The maximum insert size is < 4.4 kb.
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| Is AAV stable? What is the recommended storage temperature? |
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It is recommended to store AAV at -80˚C for long term storage. For short term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
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Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359 |