Custom CRISPR sgRNA Lentiviral Vectors & Viruses
abm carries a variety of CRISPR lentivectors and recombinant lentiviruses that will streamline your CRISPR gene editing experiments, including gene knockout, activation, repression and epigenetic modifications. Choose from our All-in-One system or our sgRNA only expression system and pair the latter with a Cas9-Expressing Stable Cell Lines or spCas9 Lentivirus. Inquire with us below to get started on your custom CRISPR project.
Advantages of the Lentivirus System:
- High infection efficiency
- Stable integration & expression of recombinant proteins
- Large insert capacity (up to 5kb)
- Ideal system for the creation of stable cell lines
- Broad host range: infection of dividing, non-dividing, stem cells, and primary cells
Service Details
Cloning Services
| Service | Vector | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – single target | pLenti-U6-sgRNA-PGK-Neo | 1.0 μg | C437 | $95.00 |
| Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – set of three targets | pLenti-U6-sgRNA-PGK-Neo | 3x1.0 μg | C441 | $250.00 |
| Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – single target | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro | 1.0 μg | C438 | $105.00 |
| Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – set of three targets | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro | 3x1.0 μg | C442 | $280.00 |
| Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – single target | pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP | 1.0 μg | C515 | $195.00 |
| Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – set of three targets | pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP | 3x1.0 μg | C516 | $465.00 |
| GFP/RFP Reporter Switch Service | 1 Service | C129 | $350.00 |
Lentivirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Custom CRISPR Lentivirus Packaging – single target | 4x500μl | 107 IU/ml | Research grade | C445 | 2-3 weeks | $395.00 |
| Custom CRISPR Lentivirus Packaging – three targets, individually packaged | 3x2ml | 107 IU/ml | Research grade | C447 | 2-3 weeks | $940.00 |
| Custom CRISPR Lentivirus Packaging – three targets, pooled packaging | 4x500μl | 107 IU/ml | Research grade | C448 | 2-3 weeks | $395.00 |
*For additional titer or volume options, please inquire.
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector /Virus |
Lentivector /Virus |
Non-Viral Vector |
Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| Browse Collection | Browse Collection | Browse Collection | Browse Collection |
Additional Resources
Documents
FAQs
| What promoter(s) do your sgRNA and All-in-One lentivectors have? |
|
sgRNAs are expressed from the U6 promoter and Cas9 is expressed from the SFFV promoter.
|
| What is the approximate time frame to generate CRISPR lentiviral constructs? |
|
The typical lead time is around 2-3 weeks for CRISPR lentivectors and 2-3 weeks for lentiviruses. Please inquire for a more accurate lead time.
|
| How should I store my lentivirus? |
|
Aliquots should be made for the lentivirus and stored at -80°C.
|
| If a high-titer custom lentivirus was ordered, is the lentiviral titration performed after a freeze-thaw or immediately after production before the freeze-down step? |
|
We do the titration after aliquoting and freeze-down. Thus, the titer should be accurate for the customer after they thaw the finished product for the first time.
|
| Which virus has your lentivirus expression system been derived from? Is it HIV? |
|
Our lentivirus expression system is derived from Human HIV-1 Virus. It employs third generation self-inactivating recombinant lentiviral vectors with enhanced biosafety features and minimal relation to wild-type Human HIV-1 Virus.
|
| How do you verify the titer? |
|
We use our LV900 series - qPCR Lentivirus Titration Kit. This kit quantifies a proprietary region of the lentiviral 5’-LTR.
|
| What is the packaging capacity for Lentivirus? |
|
The maximum insert size is <9 kb between the 5'LTR to 3'LTR.
|
Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res (2016). doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015) doi:10.1136/gutjnl-2015-309359 |
| 04 | Quarato, G et al. "Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis." Science Advances (2023) 9(21). doi: 10.1126/sciadv.adg8156 |