Trypsin
Trypsin, a proteolytic enzyme, is the standard way to detach adherent cell cultures and monolayers. This globular, pancreatic protease cleaves at the C-terminal side of lysine and arginine, breaking down vessel-adhering proteins and allowing easy resuspension during cell harvesting.
abm offers porcine-derived trypsin, EDTA-enhanced trypsin, as well as formulations of a gentle, trypsin-like enzyme. To avoid unplanned protein degradation, organic trypsin must be inactivated with trypsin inhibitors. In contrast, synthetic trypsin is deactivated via dilution, eliminating the need for additional solutions.
Product List
| Product Name | Cat. No. | Quantity | Price |
|---|---|---|---|
| Trypsin Neutralizing Solution | TM069 | 100 ml | |
| Trypsin-EDTA (0.25%) | TM050 | 500 ml | |
| Gentle Dissociation Solution | TM080 | 100 ml |
Trypsin inhibitors
Trypsin inhibitors destructively alter trypsin, nullifying the proteolytic enzyme’s ability to bind with, and so digest, proteins. Used post dissociation, trypsin inhibitors protect your cells against further protein degradation by trypsin.