Custom Baculovirus Packaging Service
abm offers a Custom Baculovirus Packaging Service which can generate titers of 106 IU/ml and 107 IU/ml using your supplied bacmid. Service includes transfection of bacmid into Sf9 cells, plaque purification and viral titering. To get started on your custom project, inquire with us below.
Advantages of the Baculovirus System:
- Ability to generate large amounts of recombinant protein.
- Can express proteins from various origins such as bacteria, virus, plants and mammals at levels from 1-500mg/L.
- Post-translational modifications are possible including phosphorylation, glycosylation and acylation.
Service Details
Custom Baculovirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer Custom Baculovirus Production | 10 ml | 106 IU/ml | Research-grade | C148 | 4-6 weeks | $750.00 |
| High Titer Custom Baculovirus Production | 2 x 200 µl | 107 IU/ml | Research-grade | C149 | 4-6 weeks | $1550.00 |
Baculovirus Controls
| Product | Unit | Cat. No. | Price |
|---|---|---|---|
| Baculovirus GFP Control Virus (106 IU/ml) | 10ml | BV001 | $695.00 |
| Baculovirus GFP Control Virus (107 IU/ml) | 2 x 200µl | BV002 | $1495.00 |
- Customer must supply >10µg for C148 and >20µg for C149 of their bacmid DNA at a concentration of >20µg/µl.
- For customer supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
Additional Info
Baculovirus is a helper-independent viral system that is used to express heterologous genes. Because of their ability to efficiently infect a wide range of hosts, and their capacity to generate high yields of gene targets, the Baculovirus Expression Vector System (BEVS) has shown considerable promise to establish long-term gene expression in most cells. Polyhedrin and p10 are considered non-essential genes when Baculovirus is in culture, allowing the coding sequences to be replaced with a sequence for the target protein. Compared to bacterial expression systems, the post-translational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.
FAQs
| What advantages does a Baculovirus expression system provide? |
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Baculovirus expression systems can be used when bacterial expression is not feasible and also when a target protein with post-translational modifications is needed for functional and structural studies.
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| What is the biosafety level for Baculovirus? |
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Baculovirus does not infect human cells and is considered safe to work with in BSL1. However, please check with your institution's Biosafety Department for more information about the rules and regulations set by your institution.
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