Key requirements for successful monoclonal antibody generation include immunization strategies, antigen design, and screening technologies. abm's novel immunization procedures and careful mouse strain selection allows for production of monoclonal antibodies which are of the utmost affinity and specificity. This is because we take great care in designing the antigens for antibody production, especially with respect to synthesizing peptides, to generate a robust antibody that is able to recognize conformational changes of the native protein under the optimum number of conditions.
Service Details
Core Services
Service Name
Description
Cat. No.
Unit
Price
Complete Package with peptide production
Peptide antigen production
Immunization (5 mice/antigen) and Sera Testing by ELISA
Fusion and Colony Screening
Subcloning, Colony Expansion and Freezing (2 clones)
(Optional) Antibody Purification by Protein A/G
C080
1 Service
Inquire
Complete Package with protein production
Protein antigen production
Immunization (5 mice/antigen) and Sera Testing by ELISA
Fusion and Colony Screening
Subcloning, Colony Expansion and Freezing (2 clones)
(Optional) Antibody Purification by Protein A/G
C487
1 Service
Inquire
Standard Package without antigen production
Immunization (5 mice/antigen) and Sera Testing by ELISA
Fusion and Colony Screening
Subcloning, Colony Expansion and Freezing (2 clones)
(Optional) Antibody Purification by Protein A/G
C087
1 Service
Inquire
Amount of antigen to submit for Standard Package Service: For peptide antigens, please submit at least 2mg. For protein antigens, please submit at least 5mg with concentrations of 0.5-1.0mg/ml.
Additional Info
Customers can provide a peptide antigen or a ready-to-inject antigen for monoclonal antibody production. When using a peptide antigen supplied by the customer, an additional charge of $250.00 is applied for KLH conjugation.
Customers have the option to start or stop the project from any stages of the mouse monoclonal antibody generation service. Customers will be responsible for the material and labour cost incurred thus far should early termination be requested.
Customer Review:
"I am a fan of abm: great product quality, great price, great rewards and great experience."
Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL
Yes, however to guarantee the antibody is effective against the native protein purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we cannot guarantee these will be effective against the native protein due to folding and other conformational issues. We only guarantee that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.
Please provide: 1) The peptide antigen design 2) Mouse monoclonal or Polyclonal Antibody Production? 3) Are you looking for the standard service or complete service? Please email this information to quotes@abmgood.com
Ko, FC et al. "PKA-induced dimerization of the RhoGAP DLC1 promotes its inhibition of tumorigenesis and metastasis." Nat Commun 4:1618 (2013). PubMed: 23511482
