Antibody Isotyping

{"id":142,"cat_no":"C233","old_cat_no":"C233","name":"Antibody Isotyping","category_id":128,"category_name":"Antibody Hybridoma Sequencing Service","frontend_category_id":0,"frontend_category_ids":null,"gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"1 Service","accession_number":"","species_id":0,"species":"","application_id":"","application":"","raised_in_id":null,"raised_in":null,"cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","source":"","unit":"","price":"125.0000","price_before_discount":"0.0000","keywords":"custom","invisible_search_keywords":"","filter_group_id":43,"filter_group_name":"Custom Services","filter_id":68,"filter_name":"Proteomic Services","search_filter_ids":"65,71","search_filter_keys":"65-71","temp_field":null,"customization":0,"filter_table_id":17584772,"filter_table_name":"","related_group_id":null,"url":"Additional-Custom-Antibody-Services.html","operator":"","status":1,"need_remove":0,"points_rule":5,"document_sort":"","price_old":"125.0000","price2024":"125.0000","created_at":null,"updated_at":"2024-09-11 02:03:06","Confirmed":"Tony Confirmed","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"Antibody-Hybridoma-Sequencing-Service.html","price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseCustomServices","geo_price":"125.00","geo_price_symbol":"$125.00","category":{"id":128,"categories":"Antibody Hybridoma Sequencing Service","pid":66,"url_key":"Antibody-Hybridoma-Sequencing-Service.html","css":null,"wid":0,"breadcrumbs":"[{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\u0022,\u0022title\u0022:\u0022Home\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/Proteomic-Service.html\u0022,\u0022title\u0022:\u0022Proteomic Services\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/Antibody-Hybridoma-Sequencing-Service.html\u0022,\u0022title\u0022:\u0022Antibody Hybridoma Sequencing Service\u0022}]","breadcrumbsIdJson":"\u003Cscript type=\u0022application\/ld+json\u0022\u003E{\u0022@context\u0022:\u0022https:\/\/schema.org\u0022,\u0022@type\u0022:\u0022BreadcrumbList\u0022,\u0022itemListElement\u0022:[{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:1,\u0022name\u0022:\u0022Home\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:2,\u0022name\u0022:\u0022Proteomic Services\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\/Proteomic-Service.html\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:3,\u0022name\u0022:\u0022Antibody Hybridoma Sequencing Service\u0022}]}\u003C\/script\u003E","breadcrumbsHtml":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\/Proteomic-Service.html\u0022\u003EProteomic Services\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022active\u0022\u003EAntibody Hybridoma Sequencing Service\u003C\/li\u003E\u003C\/ul\u003E","description":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\n\u003Cli class=\u0022abm-text-gray\u0022\u003E\u003Ca style=\u0022font-weight: bold; text-decoration: none; color: #808083;\u0022 href=\u0022\/\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca style=\u0022font-weight: bold; text-decoration: none; color: black;\u0022 href=\u0022\/Proteomic-Service.html\u0022\u003EProteomic Services\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003EAntibody Hybridoma Sequencing Service\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Ch1 id=\u0022abm-category-home-title\u0022\u003EAntibody Hybridoma Sequencing Service\u003C\/h1\u003E\n\u003Cnav id=\u0022abm-navbar\u0022 class=\u0022navbar navbar-fixed-top abm-navbar abm-affix abm-category-navbar-relative hidden-xs\u0022\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv id=\u0022abmNavbar\u0022 class=\u0022collapse navbar-collapse abm-navbar-collapse\u0022\u003E\n\u003Cul class=\u0022nav navbar-nav navbar-6\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section1\u0022\u003EOverview\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section2\u0022\u003EService Details\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section3\u0022\u003EAdditional Info\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section4\u0022\u003ERelated Products\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section5\u0022\u003EDocuments \u0026amp; FAQS\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022#abm-category-section7\u0022\u003ECitations\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/nav\u003E\n\u003Cdiv id=\u0022abm-category-section1\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-8 col-xs-12\u0022\u003E\n\u003Cp\u003EFinally obtained the desired hybridomas but do not know the antibody sequence that the cells produce? \u003Cstrong\u003Eabm\u003C\/strong\u003E offers variable region sequencing from hybridomas. Choose one of our packages below to fulfill your requirements for Patent Application, Drug Development or Cell Line Authentication. Our sequencing services cover mouse and rabbit antibodies.\u003C\/p\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/custom-service-inquiry\/?catalog=Antibody%20Hybridoma%20Sequencing%20Service%20Inquiry\u0022 rel=\u0022noopener\u0022\u003E\u003Cbutton class=\u0022custom-service-inquire-button-normal\u0022 style=\u0022float: left;\u0022\u003EInquire\u003C\/button\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv\u003E\n\u003Cp class=\u0022banners abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/catalog\/customservice\/hybridoma-sequencing\/Hybridoma-Sequencing-Service_v2.png\u0022 alt=\u0022Workflow\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-xs-12\u0022\u003E\n\u003Cp class=\u0022description-small-box-blue\u0022\u003E\u0022We are very pleased with the outcome and turnaround time. Getting accurate antibody sequences from our hybridomas allowed us to move from secreted IgG to the recombinant expression of Fabs (antigen-binding fragment) for structure determination. The sequences recovered by the Applied Biological Materials Inc. team were of high quality, unambiguous, delivered according to a pre-established schedule, and resulted in us expressing high amounts of Fab that bound to our recombinant proteins with high affinity.\u0022\u003C\/p\u003E\n\u003Ci\u003EDr. Vincent Emond, Centre de recherche du CHU de Qu\u0026eacute;bec CHUL\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section2\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3 class=\u0022abm-perfect-h3\u0022\u003EService Details\u003C\/h3\u003E\n\u003Cdiv class=\u0022table-title\u0022\u003ECore Services\u003C\/div\u003E\n\u003Cdiv\u003E\n\u003Ctable id=\u0022table1\u0022 class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003ESERVICE\u003C\/th\u003E\n\u003Cth\u003EDeliverables\u003C\/th\u003E\n\u003Cth\u003ECat.\u0026nbsp;No.\u003C\/th\u003E\n\u003Cth\u003EUNIT\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EVariable region sequencing\u003C\/td\u003E\n\u003Ctd\u003EA detailed report on 5 sequencing reactions for light chain, and 5 sequencing reactions for heavy chain\u003C\/td\u003E\n\u003Ctd\u003EC524\u003C\/td\u003E\n\u003Ctd style=\u0022color: black;\u0022\u003E1\u0026nbsp;Clone\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022C524\u0022\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003Cdiv\u003E\n\u003Ctable id=\u0022table2\u0022 class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003ESERVICE\u003C\/th\u003E\n\u003Cth\u003E1\u20114\u0026nbsp;clones\u003C\/th\u003E\n\u003Cth\u003E5\u20119\u0026nbsp;clones\u003C\/th\u003E\n\u003Cth\u003E10+\u0026nbsp;clones\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EVariable region sequencing\u003C\/td\u003E\n\u003Ctd\u003E$\u003Cspan class=\u0022customServicePriceGeoIP\u0022\u003E650\u003C\/span\u003E\/clone\u003C\/td\u003E\n\u003Ctd\u003E$\u003Cspan class=\u0022customServicePriceGeoIP\u0022\u003E550\u003C\/span\u003E\/clone\u003C\/td\u003E\n\u003Ctd style=\u0022color: black;\u0022\u003E$\u003Cspan class=\u0022customServicePriceGeoIP\u0022\u003E520\u003C\/span\u003E\/clone\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/custom-service-inquiry\/?catalog=Antibody%20Hybridoma%20Sequencing%20Service%20Inquiry\u0022 rel=\u0022noopener\u0022\u003E\u003Cbutton class=\u0022custom-service-inquire-button-normal\u0022 style=\u0022float: right;\u0022\u003EInquire\u003C\/button\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section3\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3 class=\u0022abm-perfect-h3\u0022\u003EAdditional Info\u003C\/h3\u003E\n\u003Cb class=\u0022subtitle\u0022\u003EAntibody Sequencing Advantages:\u003C\/b\u003E\n\u003Cdiv\u003E\n\u003Cul\u003E\n\u003Cli\u003EPatent application\u003C\/li\u003E\n\u003Cli\u003ETherapeutic antibody application for use in humanization\u003C\/li\u003E\n\u003Cli\u003EMass production\u003C\/li\u003E\n\u003Cli\u003ERecombinant antibody generation (using single-chain antibody (scFv))\u003C\/li\u003E\n\u003Cli\u003EElectronic banking (no need to maintain or store cells)\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Cb class=\u0022subtitle\u0022\u003Eabm advantages:\u003C\/b\u003E\n\u003Cdiv\u003E\n\u003Cul\u003E\n\u003Cli\u003EExperienced cloning and sequencing personnel\u003C\/li\u003E\n\u003Cli\u003EProjects handle by PhD-level scientists\u003C\/li\u003E\n\u003Cli\u003ESuccess at single cell level, please inquire for single cell sequencing\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003Cdiv\u003E\n\u003Cp class=\u0022banners abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/catalog\/customservice\/hybridoma-sequencing\/Single Cell Sequencing.JPG\u0022 alt=\u0022Workflow\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Cb class=\u0022subtitle\u0022\u003EWorkflow for variable region sequencing service (C524):\u003C\/b\u003E\n\u003Cdiv\u003E\n\u003Col style=\u0022list-style-type: decimal;\u0022\u003E\n\u003Cli\u003ERNA extraction from hybridoma cell line (if applicable)\u003C\/li\u003E\n\u003Cli\u003EcDNA synthesis and amplification for light chain and heavy chain, separately\u003C\/li\u003E\n\u003Cli\u003EPrimer design\u003C\/li\u003E\n\u003Cli\u003ESubcloning\u003C\/li\u003E\n\u003Cli\u003EBacterial culture and isolate plasmid from 5 colonies\/sample, for both light chain and heavy chain\u003C\/li\u003E\n\u003Cli\u003ERun 1 forward sequencing reaction per colony\u003C\/li\u003E\n\u003C\/ol\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Cb class=\u0022subtitle\u0022\u003ESample Submission Guideline:\u003C\/b\u003E\n\u003Cdiv\u003E\n\u003Ctable id=\u0022table3\u0022 class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003ESample\u0026nbsp;format\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EDetails\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETotal RNA\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; color: black;\u0022\u003E\n\u003Cul style=\u0022padding-left: 1em;\u0022\u003E\n\u003Cli\u003E\u0026gt;10 \u0026mu;l isolated RNA per sample, at the concentration of at least 1 ug\/ul\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EFrozen Cells\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; color: black;\u0022\u003E\n\u003Cul style=\u0022padding-left: 1em;\u0022\u003E\n\u003Cli\u003E1 million frozen cells per sample\u003C\/li\u003E\n\u003Cli\u003ECulture conditions of the cells\u003C\/li\u003E\n\u003Cli\u003EIf media other than IMDM, RPMI, or DMEM are used for the cells, please supply 1L of the special complete media for each sample\u003C\/li\u003E\n\u003Cli\u003ESpecial culturing plastics, if applicable\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cb class=\u0022subtitle\u0022\u003EDisclaimers and Policies\u003C\/b\u003E\n\u003Cdiv\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E does not claim any rights on any antibody sequence. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not responsible for storage of the antibody sequence beyond three months. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not responsible for storage of any reagents associated with the customer (cell-line, RNA, PCR product and cloned plasmids) after one month of the report delivery.\u003Cbr \/\u003E\u003Cbr \/\u003EA 30% non-refundable deposit is required to initiate the service. Our goal is always to deliver high-quality sequencing data to aid you in your research and downstream application. However, hybridoma sequencing by nature does not always deliver equally robust results due to a variety of factors, such as the quality of samples (low RNA content or high level of contaminates) that we receive. We will make every effort to deliver the sequencing results, although it is not possible to guarantee that it will always be achieved.\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section4\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3 class=\u0022abm-perfect-h3\u0022\u003ERelated Products\u003C\/h3\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-6 col-md-6 col-lg-6\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-xs-6 col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022serviceimages\u0022 style=\u0022text-align: left;\u0022\u003E\u003Ca href=\u0022Custom-Service-Monoclonal-Polyclonal-Antibody-Production.html\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/perfect_page\/Related-Products-tube.png\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-custom-service-image-title\u0022\u003E\u003Ca class=\u0022black-link\u0022 href=\u0022Custom-Service-Monoclonal-Polyclonal-Antibody-Production.html\u0022\u003EMouse Monoclonal Antibody Production Service\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-xs-6 col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022serviceimages\u0022 style=\u0022text-align: left;\u0022\u003E\u003Ca href=\u0022Custom-Rabbit-Polyclonal-Antibody.html\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/perfect_page\/Related-Products-tube.png\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-custom-service-image-title\u0022\u003E\u003Ca class=\u0022black-link\u0022 href=\u0022Custom-Rabbit-Polyclonal-Antibody.html\u0022\u003ERabbit Polyclonal Antibody Production Service\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-6 col-md-6 col-lg-6\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-xs-6 col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022serviceimages\u0022 style=\u0022text-align: left;\u0022\u003E\u003Ca href=\u0022Custom-Rabbit-Monoclonal-Antibody.html\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/perfect_page\/Related-Products-tube.png\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-custom-service-image-title\u0022\u003E\u003Ca class=\u0022black-link\u0022 href=\u0022Custom-Rabbit-Monoclonal-Antibody.html\u0022\u003ERabbit Recombinant Monoclonal Antibody Production\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-xs-6 col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022serviceimages\u0022 style=\u0022text-align: left;\u0022\u003E\u003Ca href=\u0022Additional-Custom-Antibody-Services.html\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/perfect_page\/Related-Products-tube.png\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-custom-service-image-title\u0022\u003E\u003Ca class=\u0022black-link\u0022 href=\u0022Additional-Custom-Antibody-Services.html\u0022\u003EImmunoprecipitation Analysis (Antigen Overexpression)\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section5\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3 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