HMC-1.3 Mast Cell Line (Clone 2E2) ; Not capable of histamine release (degranulation)
Cat. No.
T8389
Unit
1x106 cells / 1.0 ml
Price
$2,500.00
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Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.
For for-profit organizations, please contact quotes@abmgood.com for pricing.
| Cat. No. | T8389 |
| Name | HMC-1.3 Mast Cell Line (Clone 2E2) ; Not capable of histamine release (degranulation) |
| Description |
A human mast cell line was derived from HMC-1.2 cells, which express constitutively active KIT due to the presence of two oncogenic mutations: D816V and V560G. Using CRISPR technology, a site-specific mutation of V560G generated a subline, HMC-1.3, harboring a single mono-allelic D816V-KIT variant. Although HMC-1.2 and HMC-1.3 cells exhibited comparable growth in vitro, HMC-1.3 displayed significantly reduced proliferation and tumorigenicity in vivo. Phosphorylation levels of ERK1/2, AKT, and STAT5—key pathways associated with oncogenic KIT signaling—were similar between both cell lines. However, HMC-1.3 cells showed increased sensitivity to various pharmacological inhibitors, including clinically used tyrosine kinase inhibitors (TKIs) for advanced systemic mastocytosis, as well as JAK2 and BCL2 inhibitors. These findings indicate that the additional V560G-KIT mutation in HMC-1.2 cells modulates transcriptional programs driven by D816V-KIT, conferring a survival advantage, altered drug sensitivity, and enhanced tumorigenicity. This suggests that engineered human mast cells with a single D816V-KIT mutation (HMC-1.3) may serve as an improved preclinical model for mastocytosis research and drug testing. |
| Organism | Human (H. sapiens) |
| Tissue | Peripheral Blood |
| Donor History | Male, 52, Mast cell leukemia |
| Growth Properties |
Semi-adherent |
| Cell Type | Tumor Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions |
Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.
|
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Cells grow in suspension, but some cells may attach lightly to the flask. Detach cells by lightly tapping or pipetting. Change media every 3 to 4 days by splitting cultures 1:10 to 1:20. We recommend to freeze down cells from early passages, and sultures should be refreshed from earlier stock every 3 months. |
| Subculture Protocol |
1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 2x10⁶ cells/ml. Split culture 1:10 to 1:20. 2. Alternatively, collect the media with suspension cells and then detach the adherent ones with tryple express for 1-2 min. Replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions.
|
| Cryopreservation |
Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Split Ratio | 1:10-1:20 |
| Population Doubling Time (h) | 36-48 |
| Expression |
D816V-KIT mutation |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period." |
| Depositor | NIH (NIAID) |
| Application |
Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T8389 |
Print/Download Datasheet
Search CoA here
Supporting Protocol
| I want to make sure these cells express my gene of interest before I decide to buy the cell line. Can you provide a sample so this can be tested? | |
|
We do not perform extensive downstream characterization, or gene expression profiling of our cell lines. However, you can check the ‘References’ tab to see if a publication is available, or you can contact us to see if RNA or cell lysates are available for your cell line of interest. Please directly contact us for further information at quotes@abmgood.com.
|
| Does abm's PriGrow series medium contain antibiotics? | |
|
No, the medium does not contain antibiotics and needs to be supplemented by the end-user as desired.
|
| Do I need to use abm's media and Applied Cell Extracellular Matrix (G422) to culture my cells? | |
|
We strongly encourage using the recommended media and culture conditions described in the "Growth Conditions" section as they have been optimized for cell growth. Other supplier's media and coating conditions have not been tested.
|
| What is the difference between Applied Cell Extracellular Matrix (G422) and Collagen Coating? | |
|
The main component of our Applied Cell Extracellular Matrix (G422) is type I collagen specifically. For more information on abm's Applied Cell Extracellular Matrix please visit: http://www.abmgood.com/Applied-Cell-Extracellular-Matrix-G422.html.
|
| Do I have to use T25 ECM-coated flasks for growing the cells? | |
|
We strongly recommend thawing cells in the T25 flasks specified on the cell-specific product page to ensure optimal post-thaw recovery of the frozen cells before testing other culture vessels.
|
| If I want to plate these cells to multi-well plates (e.g. 96 well plates) or dishes, how should I prepare the plates? | |
| What percentage of Trypsin-EDTA should be used to subculture cells? Can I use Trypsin-EDTA containing phenol red? | |
|
The standard concentration used for subculturing is 0.25% Trypsin-EDTA, available at abm (Cat. No. TM050). Yes, Trypsin-EDTA containing phenol red can be used as it does not interfere with trypsinization.
|
| How often do I need to change the media? | |
|
Media should be changed every 2-3 days or as specified within the recommended growth conditions.
|
| Why are these cells classified as biosafety level II? | |
|
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
|
| How long can I store frozen vials? | |
|
Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen vapor phase (or below -130°C) indefinitely without affecting viability.
|
| What is the recommended storage temperature for cells? | |
|
In general, if you received:
|
| Can I substitute heat-inactivated FBS with FBS or vice versa? | |
|
FBS and heat-inactivated FBS are different in their composition; they cannot be substituted for one another.
|
| My cells are not detaching, what method do you recommend to trypsinize the cells? | |
|
| How are live cells shipped? | |
|
Live cells are shipped as a live culture in a T12.5 flask. Cells are seeded at an optimal confluency to avoid metabolic waste build up during transport. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
|
| Why are cells not attaching and forming clumps after subculturing with trypsin? | |
|
Cells form clumps and display attachment issues when the trypsinization agent is not effectively neutralized. To completely neutralize the trypsin agent, add an equal amount of complete growth media prior to centrifugation. Subtle changes in culture conditions, particularly in pH and the quality of serum used in the growth medium, may also affect the amount of clumping exhibited by the cells.
|
| Why are my cells forming clumps after plating? | |
|
It is important to ensure cell clumps are broken up through gentle re-suspension by pipetting up and down several times after pelleting cells by centrifugation. This will ensure a homogenous single cell suspension for even plating.
|
| Do I need to add the selection drug to the complete growth medium? | |
|
We recommend maintaining selection pressure using the drug concentration specified in the Growth Conditions section.
|
| Where can I find the CoA for my product? | |
|
Certificates of analysis can be found here (https://www.abmgood.com/CoA-Library.html).
|
-
Butterfield, J. H., Weiler, D., Dewald, G., & Gleich, G. J. (1988). Establishment of an immature mast cell line from a patient with mast cell leukemia. Leukemia research, 12(4), 345–355. https://doi.org/10.1016/0145-2126(88)90050-1
Bandara, G., Falduto, G. H., Luker, A., Bai, Y., Pfeiffer, A., Lack, J., Metcalfe, D. D., & Olivera, A. (2023). CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis. Frontiers in immunology, 14, 1078958. https://doi.org/10.3389/fimmu.2023.1078958
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margin-bottom: 0;\n }\n\n .search-sub-filter-item-clear {\n display: none;\n }\n\n .search-sub-filter-item.active .search-sub-filter-item-clear,\n .search-sub-filter-item.selected .search-sub-filter-item-clear {\n display: flex;\n align-items: center;\n position: absolute;\n top: 0;\n right: -1px;\n height: 100%;\n padding: 0 10px;\n background-color: #333;\n color: #fff;\n }\n\n .search-clear {\n text-align: right;\n }\n\n .search-clear button {\n padding: 8px 15px;\n border: 1px solid #EF6331;\n background-color: #EF6331;\n color: #fff;\n border-radius: 4px;\n cursor: pointer;\n opacity: 1;\n transition: .3s;\n }\n\n .search-clear button:not(:disabled):hover {\n opacity: .8;\n }\n\n .search-clear button:disabled {\n opacity: .5;\n cursor: not-allowed;\n }\n\n .result-box {\n margin-top: 50px;\n }\n\n #result-container {\n padding-top: 20px;\n }\n\n #result-container ul {\n list-style: none;\n padding: 0;\n }\n\n .search-result-notice {\n background-color: #f2f2f2;\n padding: 15px 20px;\n margin-bottom: 20px;\n border-radius: 6px;\n }\n\n\n \/* Radio *\/\n .abm-radio {\n display: flex;\n margin-right: 15px;\n align-items: center;\n }\n\n .abm-radio input {\n display: none;\n }\n\n .abm-radio .abm-radio-icon {\n display: block;\n width: 1em;\n height: 1em;\n border-radius: 50%;\n border: 2px solid #ccc;\n margin-right: 5px;\n position: relative;\n }\n\n .abm-radio .abm-radio-text {\n color: #333;\n font-weight: 700;\n }\n\n .abm-radio:hover .abm-radio-icon,\n .abm-radio input:checked+.abm-radio-icon {\n border-color: #EF6331;\n }\n\n .abm-radio:hover .abm-radio-text {\n color: #EF6331;\n }\n\n .abm-radio input:checked+.abm-radio-icon::after {\n content: \u0022\u0022;\n display: block;\n width: 0.5em;\n height: 0.5em;\n background-color: #EF6331;\n border-radius: 50%;\n position: absolute;\n top: 50%;\n left: 50%;\n transform: translate(-50%, -50%);\n }\n\n .abm-radio input:checked+.abm-radio-icon+.abm-radio-text {\n color: #EF6331;\n }\n\n .loading {\n display: flex;\n position: absolute;\n left: 50%;\n top: 50%;\n transform: translate(-50%, -50%);\n }\n\n .loading-item {\n width: 5px;\n height: 20px;\n background-color: #EF6331;\n\n -webkit-animation: loading 1.2s infinite ease-in-out;\n animation: loading 1.2s infinite ease-in-out;\n }\n\n .loading-item+.loading-item {\n margin-left: 5px;\n }\n\n .loading-item:nth-child(2) {\n -webkit-animation-delay: .1s;\n animation-delay: .1s;\n }\n\n .loading-item:nth-child(3) {\n -webkit-animation-delay: .2s;\n animation-delay: .2s;\n }\n\n .loading-item:nth-child(4) {\n -webkit-animation-delay: .3s;\n animation-delay: .3s;\n }\n\n .loading-item:nth-child(5) {\n -webkit-animation-delay: .4s;\n animation-delay: .4s;\n }\n\n @-webkit-keyframes loading {\n\n 0%,\n 40%,\n 100% {\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @keyframes loading {\n\n 0%,\n 40%,\n 100% {\n transform: scaleY(1);\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n transform: scaleY(1.7);\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @media screen and (max-width: 768px) {\n .search-sub-filter-row {\n grid-template-columns: 1fr;\n grid-gap: 15px;\n }\n\n .search-sub-filter-items {\n height: unset;\n border-right: 1px solid #ddd;\n }\n\n .abm-search-item-action {\n display: none;\n }\n }\n\u003C\/style\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const API = \u0027\/product\/searchApi\u0027;\n\n const DEFAULT_FILTER_ID = 15;\n\n function jump(params = {}, id = \u0027search-box\u0027) {\n let url = generateURL(params, location.origin + location.pathname);\n if (id) {\n scrollToID(id);\n }\n history.pushState(params, \u0027\u0027, url);\n search();\n }\n\n function scrollToID(id) {\n const el = document.getElementById(id);\n if (el) {\n window.scrollTo(0, getOffsetTop(el));\n }\n }\n\n function getOffsetTop(el) {\n let result = el.offsetTop;\n if (el.offsetParent) {\n result += getOffsetTop(el.offsetParent);\n }\n return result;\n }\n\n function getParams(state) {\n const params = [];\n for (const key in state) {\n params.push(`${key}=${state[key]}`);\n }\n return params;\n }\n\n function generateURL(state, base = API) {\n const params = getParams(state);\n if (params \u0026\u0026 params.length) {\n base += \u0027?\u0027 + params.join(\u0027\u0026\u0027);\n }\n return base;\n }\n\n function getDefaultFilterId() {\n return getSearchValueByKey(\u0027filter_id\u0027, DEFAULT_FILTER_ID);\n }\n\n function getSearchValueByKey(key, defaultVal = \u0027\u0027) {\n const search = location.search;\n if (search) {\n const str = search.substring(1);\n const arr = str.split(\u0027\u0026\u0027);\n for (const item of arr) {\n const keyValue = item.split(\u0027=\u0027);\n if (keyValue[0] === key) {\n return keyValue[1];\n }\n }\n }\n return defaultVal;\n }\n\n function search() {\n const historyStateExists = !!history.state;\n\n let state = history.state;\n if (!state) {\n state = { filter_id: getDefaultFilterId() };\n } else if (!state.filter_id) {\n state.filter_id = getDefaultFilterId();\n }\n\n for (const item of [\u0027species_id\u0027, \u0027tissue_system\u0027, \u0027tissue_id\u0027, \u0027page\u0027]) {\n const value = getSearchValueByKey(item);\n if (!state[item] \u0026\u0026 value) {\n state[item] = value;\n }\n }\n\n if (!historyStateExists) {\n history.pushState(Object.assign({}, state), \u0027\u0027);\n }\n\n disableFilterSection();\n fetch(generateURL(state)).then(res =\u003E res.json()).then(res =\u003E {\n if (0 === res.code) {\n renderProducts(res.data.products, res.data.page);\n renderSubFilter({\n organism: res.data.species,\n tissue_system: res.data.tissue_system,\n tissue_id: res.data.tissue\n });\n renderPaginate(res.data.page, res.data.lastPage);\n\n const filterId = state.filter_id;\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.checked = el.value + \u0027\u0027 === filterId + \u0027\u0027;\n });\n }\n\n enableFilterSection();\n }).catch(() =\u003E {\n enableFilterSection();\n });\n }\n\n function renderProducts(products, currentPage = 1) {\n let html = \u0027\u0027;\n if (currentPage \u003E 1) {\n html += \u0027\u003Cdiv class=\u0022search-result-notice\u0022\u003EProduct not found? Click \u003Ca href=\u0022javascript:;\u0022 onclick=\u0022scrollToID(\\\u0027search-box\\\u0027)\u0022\u003Ehere\u003C\/a\u003E to refine the result.\u003C\/div\u003E\u0027;\n }\n html += \u0027\u003Cul\u003E\u0027;\n for (const product of products) {\n const url = getProductUrl(product);\n const price = getProductPrice(product);\n\n const mediaPart = generateMediaPart(product);\n const categoryPart = generateCategoryPart(product);\n\n html += `\n \u003Cli class=\u0022abm-search-item\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-container\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-header\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-title\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-name\u0022\u003E\n \u003Ca class=\u0022abm-link\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003E${product.name}\u003C\/a\u003E\n \u003C\/div\u003E\n ${categoryPart}\n \u003C\/div\u003E\n \u003Cdiv class=\u0022abm-search-item-action\u0022\u003E\n \u003Ca class=\u0022btn abm-btn btn-block\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003EView Product\u003C\/a\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003Chr class=\u0022abm-search-item-hr\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-body\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-content\u0022\u003E\n \u003Cul class=\u0022abm-search-item-content-base\u0022\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003ECat. No.:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.cat_no}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EPrice:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${price}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EUnit:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.unit_quantity}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003C\/ul\u003E\n \u003C\/div\u003E\n ${mediaPart}\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n document.querySelector(\u0027#result-container\u0027).innerHTML = html;\n }\n\n function getProductUrl(product) {\n if (product.seo \u0026\u0026 product.seo.url_key) {\n return \u0027\/\u0027 + ltrim(product.seo.url_key, \u0027\/\u0027);\n }\n\n const type = product.model_type.substr(22).toLowerCase();\n return \u0027\/catalog\/products\/\u0027 + product.id + \u0027\/type\/\u0027 + type;\n }\n\n function getProductPrice(product) {\n return isNaN(Number(product.geo_price)) ? (product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? (\u0027\u003Cstrong\u003E\u0027 + item.value + \u0027\u003C\/strong\u003E\u0027) : item.value}\u003C\/span\u003E\n \u003Cdiv class=\u0022search-sub-filter-item-clear\u0022\u003EX\u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n return html;\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-container\u0027).innerHTML = html;\n\n eventListener(\u0027#paginate-container a\u0027, \u0027click\u0027, handlePaginateClick);\n }\n\n function handlePaginateClick() {\n if (this.parentNode.classList.contains(\u0027disabled\u0027)) {\n return;\n }\n const state = history.state || {};\n state.page = this.dataset.page;\n jump(state, \u0027result-box\u0027);\n }\n\n function disableFilterSection() {\n const el = document.querySelector(\u0027.search-box\u0027);\n el.style.position = \u0027relative\u0027;\n addShadow(el, \u0027shadow-search-box\u0027, true);\n\n const elResult = document.querySelector(\u0027.result-box\u0027);\n elResult.style.position = \u0027relative\u0027;\n addShadow(elResult, \u0027shadow-result-box\u0027);\n\n document.querySelector(\u0027.search-clear button\u0027).disabled = false;\n }\n\n function addShadow(elem, id, addLoading = false) {\n const el = document.getElementById(id);\n if (el) {\n el.style.display = \u0027block\u0027;\n el.style.opacity = 1;\n return;\n }\n\n elem.insertAdjacentHTML(\u0027beforeEnd\u0027, genearteShadow(id, addLoading));\n }\n\n function removeShadow(id) {\n const el = document.getElementById(id);\n if (el) {\n el.style.opacity = 0;\n setTimeout(function () {\n el.style.display = \u0027none\u0027;\n }, 500);\n }\n }\n\n function genearteShadow(id, addLoading = false) {\n let html = `\u003Cdiv id=\u0022${id}\u0022 style=\u0022position: absolute; left: 0; top: 0; right: 0; bottom: 0; background-color: rgba(255, 255, 255, .9); z-index: 100; transition: .5s;\u0022\u003E`;\n if (addLoading) {\n html += \u0027\u003Cdiv class=\u0022loading\u0022\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027\n }\n html += `\u003C\/div\u003E`;\n\n return html;\n }\n\n function enableFilterSection() {\n removeShadow(\u0027shadow-search-box\u0027);\n removeShadow(\u0027shadow-result-box\u0027);\n document.querySelector(\u0027.search-clear button\u0027).disabled = !document.querySelector(\u0027.search-sub-filter-item.active\u0027);\n }\n\n search();\n\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.addEventListener(\u0027change\u0027, function (e) {\n if (el.classList.contains(\u0027prevent\u0027)) {\n return false;\n }\n\n document.querySelector(\u0027input[name=\u0022search-filter_id\u0022][value=\u0022\u0027 + DEFAULT_FILTER_ID + \u0027\u0022]\u0027).checked = true;\n\n switch (el.value + \u0027\u0027) {\n case \u002714\u0027:\n location.href = \u0027\/Immortalized-Cell-Lines.html\u0027;\n break;\n case \u002715\u0027:\n location.href = \u0027\/Tumor-Cell-Lines.html\u0027;\n break;\n case \u002716\u0027:\n location.href = \u0027\/Primary-Cells.html\u0027;\n break;\n default:\n jump({ filter_id: el.value });\n }\n });\n });\n\n document.querySelector(\u0027.search-clear button\u0027).addEventListener(\u0027click\u0027, () =\u003E {\n jump({\n filter_id: getDefaultFilterId()\n });\n });\n\n window.onpopstate = function () {\n \/\/ \u76f4\u63a5\u8fd4\u56de\u4e0a\u4e00\u7ea7\u9875\u9762\n history.back();\n return false;\n if (!history.state) {\n history.back();\n }\n search();\n }\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"Tumor Cell","meta_keywords":"tumor cell lines, abm, cellular materials, cancer research, cell biology, immortalized cells, primary cells, KSTAR, SUNE2, nasopharyngeal carcinoma","meta_description":"abm offers a wide range of tumor cell lines derived from various tissues and species for cancer research and cell biology applications. Browse our collection of immortalized, primary, and tumor cells online.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":"abm_catalog_cellular","image":null,"independentPage":0,"top_type":1,"sort_order":203,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-02-24 00:20:51"},"info":{"id":29548,"cat_no_base":"T8389","parent_id":77134,"image":null,"price-original":null,"description":"\u003Cp\u003EA human mast cell line was derived from HMC-1.2 cells, which express constitutively active KIT due to the presence of two oncogenic mutations: D816V and V560G. Using CRISPR technology, a site-specific mutation of V560G generated a subline, \u003Cstrong\u003EHMC-1.3\u003C\/strong\u003E, harboring a single \u003Cstrong\u003Emono-allelic D816V-KIT variant\u003C\/strong\u003E.\u003C\/p\u003E\n\u003Cp\u003EAlthough HMC-1.2 and HMC-1.3 cells exhibited comparable growth in vitro, HMC-1.3 displayed significantly reduced proliferation and tumorigenicity in vivo. Phosphorylation levels of \u003Cstrong\u003EERK1\/2, AKT, and STAT5\u0026mdash;key pathways associated with oncogenic KIT signaling\u003C\/strong\u003E\u0026mdash;were similar between both cell lines. However, HMC-1.3 cells showed increased sensitivity to various pharmacological inhibitors, including clinically used tyrosine kinase inhibitors (TKIs) for advanced systemic mastocytosis, as well as JAK2 and BCL2 inhibitors.\u003C\/p\u003E\n\u003Cp\u003EThese findings indicate that the additional \u003Cstrong\u003EV560G-KIT mutation in HMC-1.2 cells modulates transcriptional programs driven by D816V-KIT\u003C\/strong\u003E, conferring a \u003Cstrong\u003Esurvival advantage, altered drug sensitivity, and enhanced tumorigenicity\u003C\/strong\u003E. This suggests that engineered human mast cells with a single D816V-KIT mutation (HMC-1.3) may serve as an improved preclinical model for mastocytosis research and drug testing.\u003C\/p\u003E","shipment_type":null,"internal_leadtime":null,"lysate_type":null,"small_image":null,"growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":null,"family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"IMDM + 10% FBS + 2 mM L-Glutamine + 1% P\/S","alias":null,"tier_price":null,"operator_no_use":null,"addtime":null,"cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":null,"expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":null,"cell_morphology_specification":null,"tax_class_id":null,"inventory_location":null,"vector":null,"internal_note":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap-mode: wrap;\u0022\u003EVAN 2024-11-21: Morphology update from Jovita: Cells are not fully suspension but are\u0026nbsp;\u003C\/span\u003E\u003Cspan style=\u0022text-wrap-mode: wrap;\u0022\u003Ehalf suspension and half adherent.\u003C\/span\u003E\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":null,"seeding_density_ml":null,"source":null,"source_catno":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap-mode: wrap;\u0022\u003EOnly sell to academic. Cannot be used in Industry Sponsored Research. Commercial Entities must obtain license before purchasing from abm, direct to licensing@abmgood.com.\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":"36-48","source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":null,"donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":null,"biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003ESemi-adherent\u003C\/p\u003E","expression_profile":null,"storage":null,"propagation_method":null,"usage":null,"freeze_thaw_recovery":null,"str_profile":null,"str_markers":"","subculture_protocol":"\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E1. Simply add fresh complete media directly to the culture. \u003Cstrong\u003ED\u003C\/strong\u003E\u003Cstrong\u003Eo not allow cell density to exceed 2x10\u2076 cells\/ml. Split culture 1:10 to 1:20.\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E2. Alternatively,\u0026nbsp;collect the media with suspension cells and then detach the adherent ones with tryple express for 1-2 min.\u0026nbsp;Replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E3. Incubate the cells at the recommended conditions.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","preservation_protocol":null,"passage_number":null,"qc":null,"shipping_conditions":"\u003Cp\u003EShip with dry ice.\u003C\/p\u003E","disclaimer_general":"\u003Cp\u003E1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and procedures. The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period.\u0022\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":"NIH (NIAID)","licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":null,"internal_product_note":null,"donor_history":"Male, 52, Mast cell leukemia","split_ratio":"1:10-1:20","expression":"\u003Cp\u003ED816V-KIT mutation\u003C\/p\u003E","growth_conditions":"\u003Cp\u003E\u003Cstrong style=\u0022color: rgb(255, 0, 0); text-wrap: wrap;\u0022\u003E\u003Cstrong\u003EPriCoat\u2122 T25 Flasks (\u003Ca href=\u0022https:\/\/www.abmgood.com\/pricoat-t25-flasks.html\u0022 target=\u0022_self\u0022\u003EG299\u003C\/a\u003E) are recommended for optimal cell culture.\u003C\/strong\u003E\u0026nbsp;\u003C\/strong\u003E Iscove\u2019s Modified Dulbecco\u2019s Medium (IMDM) (\u003Ca href=\u0022https:\/\/www.abmgood.com\/Iscoves-Modified-Dulbeccos-Medium-IMDM-.html\u0022 target=\u0022_self\u0022\u003ETM505\u003C\/a\u003E) + 10% FBS(Regular*) + 2 mM L-Glutamine (\u003Ca href=\u0022https:\/\/www.abmgood.com\/l-glutamine-g275.html\u0022 target=\u0022_self\u0022\u003EG275\u003C\/a\u003E) + 1% Penicillin\/Streptomycin Solution (\u003Ca href=\u0022https:\/\/www.abmgood.com\/penicillin-streptomycin-solution-g255.html\u0022 target=\u0022_self\u0022 style=\u0022text-wrap: wrap;\u0022\u003EG255\u003C\/a\u003E), 37.0\u00b0C, 5% CO\u2082.\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Ci\u003E*Do not heat-inactivate\u003C\/i\u003E\u003C\/p\u003E","recommend":"\u003Cp\u003EWorried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":"\u003Cp style=\u0022text-wrap-mode: wrap;\u0022\u003EButterfield, J. H., Weiler, D., Dewald, G., \u0026amp; Gleich, G. J. (1988). Establishment of an immature mast cell line from a patient with mast cell leukemia. Leukemia research, 12(4), 345\u2013355.\u0026nbsp;\u003Ca href=\u0022https:\/\/doi.org\/10.1016\/0145-2126(88)90050-1\u0022\u003Ehttps:\/\/doi.org\/10.1016\/0145-2126(88)90050-1\u003C\/a\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp style=\u0022text-wrap-mode: wrap;\u0022\u003E\u003Cbr\/\u003E\u003C\/p\u003E\u003Cp style=\u0022text-wrap-mode: wrap;\u0022\u003EBandara, G., Falduto, G. H., Luker, A., Bai, Y., Pfeiffer, A., Lack, J., Metcalfe, D. D., \u0026amp; Olivera, A. (2023). CRISPR\/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis. Frontiers in immunology, 14, 1078958.\u0026nbsp;\u003Ca href=\u0022https:\/\/doi.org\/10.3389\/fimmu.2023.1078958\u0022\u003Ehttps:\/\/doi.org\/10.3389\/fimmu.2023.1078958\u003C\/a\u003E\u0026nbsp;\u003C\/p\u003E","cryopreservation":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003ECryopreservation Medium (\u003C\/span\u003E\u003Ca style=\u0022text-wrap: wrap;\u0022 href=\u0022https:\/\/www.abmgood.com\/cryopreservation-medium-tm024.html\u0022 target=\u0022_self\u0022\u003ETM024\u003C\/a\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E), or complete growth media with 10% DMSO.\u003C\/span\u003E\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","promotions":null,"sync_images":null,"thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003ECells grow in suspension, but some cells may attach lightly to the flask. Detach cells by lightly tapping or pipetting.\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EChange media every 3 to 4 days by splitting cultures 1:10 to 1:20.\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EWe recommend to freeze down cells from early passages, and sultures should be refreshed from earlier stock every 3 months.\u003C\/strong\u003E\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2024-12-18 02:02:01","updated_at":"2025-02-13 18:57:39","concentration":null},"seo":{"id":1216220,"catalog_id":77134,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"HMC-1.3-Mast-Cell-Line-Clone-2E2.html","meta_title":null,"meta_keywords":null,"meta_description":null,"created_at":"2025-02-13 18:03:54","updated_at":"2025-02-13 18:05:22"},"gene":null,"media":[{"id":388222,"parent_id":77134,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/vyxEdpsUHiiekz5rJyl9BNY8RSF9yQ9vjEi0zKHz.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 20:47:08","updated_at":"2025-02-13 20:47:08"},{"id":388223,"parent_id":77134,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/X2HEDpEMXfg4IjJxvOsCoVqAx7tGRuItqpzojKdp.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 20:47:13","updated_at":"2025-02-13 20:47:13"}]}