Human Mast Cell Line (LAD2) ; Capable of histamine release (degranulation)

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(product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? 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Browse our collection of immortalized, primary, and tumor cells online.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":"abm_catalog_cellular","image":null,"independentPage":0,"top_type":1,"sort_order":203,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-02-24 00:20:51"},"info":{"id":7785,"cat_no_base":"T8157","parent_id":54479,"image":null,"price-original":null,"description":"\u003Cp\u003EThe LAD2 human mast cell line originates from bone marrow aspirates of a patient with mast cell sarcoma\/leukemia. These cells exhibit ultrastructural characteristics of human mast cells and express \u003Cstrong\u003EFc\u0026epsilon;RI\u003C\/strong\u003E, along with markers such as \u003Cstrong\u003ECD4, 9, 13, 14, 22, 31, 32, 45, 64, 71, 103, 117, 132, CXCR4 (CD184), and CCR5 (CD195)\u003C\/strong\u003E. Additionally, they contain intracytoplasmic histamine, tryptase, and chymase. Notably, \u003Cstrong\u003ELAD2 cells do not carry activating mutations at codon 816 of the \u003Cem\u003Ec-kit\u003C\/em\u003E gene\u003C\/strong\u003E. Similar to LAD1, LAD2 cells release \u0026beta;-hexosaminidase upon Fc\u0026epsilon;RI or Fc\u0026upsih;RI aggregation.\u003C\/p\u003E\n\u003Cp\u003EThe availability of the LAD2 mast cell line provides a unique opportunity to study the biology of human mast cells. Over time, it has become the most widely used and recognized gold-standard mast cell line for allergy and mastocytosis research, as well as a crucial tool in pharmaceutical development.\u003C\/p\u003E","shipment_type":null,"internal_leadtime":null,"lysate_type":null,"small_image":null,"growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003EThis novel, mast cell line (LAD2) responds to human stem cell factor (SCF) and has functional Fc\u03b5RI and FcyRI receptors. Alongside expressing Fc\u03b5RI, CD4, 9, 13, 22, 45, 64, 71, 103, 117, 132, CCR5 and CXCR4, these cells also express CD14, 31 and 32, to a lesser degree. Cells are susceptible T-tropic, M-tropic, and dual tropic HIV infection. Dervied from CD34+ cells, these cells are suitable for research relating to human mast cell survival and proliferation, as well as activation through the gamma and epsilon receptors.\u0026nbsp;\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"**Check handling protocol and ensure non-DMSO, non-FBS cryopreservative is available before working with cells**850 rpm for 5 minutes*Stempro-34 SFM (Thermo Fisher Scientific # 10639011) + Stempro supplement + 100ng\/ml hSCF (R \u0026 D Systems # 255-SC\/CF) + 2mM L-Glutamine + 1% P\/S**Normal to observe cell death in the first two weeks after thawing.***5 % DMSO 20% FBS and 75 % culture media**","alias":null,"tier_price":null,"operator_no_use":null,"addtime":null,"cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":"Rounded, granulated; some large aggregates at higher densities","expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":null,"cell_morphology_specification":null,"tax_class_id":null,"inventory_location":null,"vector":null,"internal_note":"\u003Cp\u003E***Only sell to academic*** Cannot be used in Industry Sponsored Research.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E**No live cells unless its paid - cells are too sensitive**\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E**\u003C\/span\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EMast Cell Degranulation Assay Kit :\u0026nbsp;\u003C\/span\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003ETribioscience (\u003C\/span\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003ETBS2101)\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp\u003E********************************\u0026nbsp;Special MTA Required **************************************\u003C\/p\u003E\n\u003Cp\u003E***************************** Ethical Statement from Licensor******************************\u003C\/p\u003E\n\u003Cp\u003EThe LAD2 cells were collected according to 45 CFR Part 46, Protection of Human Subjects, with assurance number# 00005897. If you need more information, please get in touch with Olivera, Ana (NIH\/NIAID) [E] ana.olivera@nih.gov.\u003C\/p\u003E\n\u003Cp\u003E---------------------------------------------------------------------------------------------------------------\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003ECells are\u0026nbsp;extremely sensitive to mycoplasma contamination.\u0026nbsp;Cells are passaged for less than a year before they start losing their functionality.\u0026nbsp;\u0026nbsp;Functionality is determined every 2 months using a degranulation assay (protocol in folder).\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E2024-10-11: Made edits to the website to make the instructions clearer for end users. More info on the change is logged in the cell folder.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003EJune 14, 2023 -\u0026nbsp;\u003C\/p\u003E\n\u003Cul class=\u0022 list-paddingleft-2\u0022 style=\u0022font-family: \u0027Microsoft YaHei UI\u0027; font-size: 14px; text-wrap: wrap; background-color: #ffffff;\u0022\u003E\n\u003Cli\u003E\n\u003Cp\u003EThaw in small vessel (T12.5 or T25 max) after a spin down\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003ECells are sensitive after thaw, leave them alone for 3-5 days minimum, some death will occur but this is normal, licensor confirmed it.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EThey can be FD in 5% DMSO (she did extensive QC on it)\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003ETM018 + 10% FBS + 1% P\/S looks like a promising alternate media and she is doing final QC on the cells with that media in the next 1-2 weeks.`\u0026nbsp;\u003C\/p\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003EPublication for troubleshooting cells:\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003ER\u0026aring;dinger, M., Jensen, B. M., Kuehn, H. S., Kirshenbaum, A., \u0026amp; Gilfillan, A. M. (2010). Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood. Current protocols in immunology, Chapter 7, Unit\u0026ndash;7.37. https:\/\/doi.org\/10.1002\/0471142735.im0737s90\u003C\/p\u003E\n\u003Cp\u003E************************************** Lot Passage No. *************************************\u003C\/p\u003E\n\u003Cp\u003EC23E08JA: P=U+2 |\u0026nbsp;C23S20GA: P=U+4 | C23S18GA: P=U+5\u0026nbsp;|\u0026nbsp;C24S19JCN: P=U+6\u0026nbsp;| C24S23JANB: P=U+6\u0026nbsp;| C24C04JANB: P=U+6\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: nowrap;\u0022\u003EC23E08JA: FD in 5% DMSO | C24S19JCNB: FD in 5% DMSO + 20% FBS+ 75% CM\u0026nbsp;|\u0026nbsp;C24S23JANB and C24C04JANB: FD in CyroScarless (bioverde) with NO DMSO and FBS\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: nowrap;\u0022\u003Esome cell death observed in first week upon thaw - debris will be noted.\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp\u003EB24N14HAN: P= U+P5 \u0026nbsp; \u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: nowrap;\u0022\u003E\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":null,"seeding_density_ml":"500,000-800,000","source":null,"source_catno":"\u003Cp\u003E$1495 **ACADEMIC CUSTOMERS ONLY, Special MTA (no major edits allowed.\u003C\/p\u003E\u003Cp\u003EIf Commercial End User obatins license with NIH, abm to send the Special Commercial MTA to be executed, and can only release the cells once NIH send authorization to release material.\u003C\/p\u003E","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":"Initially grow slowly; double in 2-4 weeks under optimal conditions.","source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":"**No Live Cells**","donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":null,"biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003ESuspension; Rounded, granulated; some large aggregates at higher densities\u003C\/p\u003E","expression_profile":null,"storage":null,"propagation_method":"\u003Cp\u003EPriGrow X Series Medium (TM8157) + 100ng\/ml hSCF + 2mM L-Glutamine + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u0026deg;C, 5% CO\u2082.\u003C\/p\u003E\n\u003Cp\u003EReplace half of the medium weekly with an equal volume of fresh medium. Do not allow cells to become over-confluent. Cells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed.\u003C\/p\u003E\n\u003Cp\u003EDegranulation assays must be conducted every 2 months to test functionality (protocol can be found under the Documents tab).\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-decoration: underline;\u0022\u003EThawing Cells:\u003C\/span\u003E\u003Cbr \/\u003EThaw vial according to regular thawing protocols. Wash cells in growth media to remove CryoScarless and place in T25 with 10 mL of culture medium. Post-thaw viability should be 80-95%. Remove debris during the first week post-tha by skimming, where possible. Hemidepletion should be performed weekly. Expanding cultures should be transferred to T75 or larger flask as required.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-decoration: underline;\u0022\u003EInstructions for Freezing and Storing Cells:\u003C\/span\u003E\u003Cbr \/\u003EFollowing centrifugation, leave a small amount of culturing media (50-100 uL). Suspend cells in non-DMSO, non-FBS containing CryoScarless Cryopreservative (BioVerde) at a final concentration of 5x10\u0026lt;sup\u0026gt;6\u0026lt;\/sup\u0026gt; cells\/mL. Place vials in a cryo container and store at -80\u0026deg;C or in liquid nitrogen for longer preservation.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","usage":null,"freeze_thaw_recovery":null,"str_profile":null,"str_markers":"{\u0022D5S818\u0022:\u002210,11\u0022,\u0022D13S317\u0022:\u002210,12\u0022,\u0022D7S820\u0022:\u002211,11\u0022,\u0022D16S539\u0022:\u002211,13\u0022,\u0022VWA\u0022:\u002215,18\u0022,\u0022TH01\u0022:\u00227,7\u0022,\u0022AMEL\u0022:\u0022X,Y\u0022,\u0022TPOX\u0022:\u002211,11\u0022,\u0022CSF1PO\u0022:\u00229,10\u0022,\u0022D12S391\u0022:\u002218,22\u0022,\u0022FGA\u0022:\u002223,24\u0022,\u0022D2S1338\u0022:\u002217,21\u0022,\u0022D21S11\u0022:\u002232.2,33.2\u0022,\u0022D18S51\u0022:\u002213,14\u0022,\u0022D8S1179\u0022:\u002213,14\u0022,\u0022D3S1358\u0022:\u002216,19\u0022,\u0022D6S1043\u0022:\u002212,14\u0022,\u0022PENTAE\u0022:\u00225,10\u0022,\u0022D19S433\u0022:\u002213,15\u0022,\u0022PENTAD\u0022:\u002213,14\u0022,\u0022D1S1656\u0022:\u002215,17.3\u0022}","subculture_protocol":"\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E1. Transfer expanding cultures to T75 or larger culture vessels to keep cell densities between 5x10\u003Csup\u003E5\u003C\/sup\u003E\u0026nbsp;-\u0026nbsp;\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E8x10\u003C\/span\u003E\u003Csup style=\u0022text-wrap: wrap;\u0022\u003E5\u003C\/sup\u003E\u003Csup\u003E\u0026nbsp;\u003C\/sup\u003Ecells\/ml. Do not exceed a density of 1x10\u003Csup\u003E6\u003C\/sup\u003E cells\/ml.\u0026nbsp;\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E2.\u0026nbsp;Remove half the volume of the culture media and replace with fresh media (hemidepletion) the first or second week after thawing. Remove debris by skimming media, if possible. It is normal to see increased cell death for the first 2-3 weeks following thawing.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E3. Alternatively, collect cells and centrifuge (850 rpm for 5 min ) to form a pellet. Discard half the supernatant and re-suspend the cell pellet in the remaining media. Add fresh complete media in 1:1 ratio and aliquot the cell suspension to new culture vessels, as desired. \u003Cstrong\u003EA complete media change is not advised\u003C\/strong\u003E.\u0026nbsp;\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E4. Incubate the cells at the recommended conditions.\u003C\/p\u003E","preservation_protocol":null,"passage_number":null,"qc":null,"shipping_conditions":"\u003Cp\u003EShip with dry ice.\u003C\/p\u003E","disclaimer_general":"\u003Cp\u003E1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and procedures. The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period.\u0022\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":"NIH (NIAID)","licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":null,"internal_product_note":"\u003Cp\u003E**SPECIAL MTA**\u003C\/p\u003E","donor_history":"Male, 44, Mastocytosis","split_ratio":null,"expression":"\u003Cp\u003EFc\u0026epsilon;RI, CD33, CD34, CD63, CD117, CD133, CD193\u003C\/p\u003E","growth_conditions":"\u003Cp\u003E\u003Cspan style=\u0022color: rgb(255, 0, 0);\u0022\u003E\u003Cstrong\u003EPriCoat\u2122 T25 Flasks (\u003Ca href=\u0022https:\/\/www.abmgood.com\/pricoat-t25-flasks.html\u0022 target=\u0022_self\u0022\u003EG299\u003C\/a\u003E) are recommended for optimal cell culture.\u003C\/strong\u003E\u003C\/span\u003E PriGrow X Series Medium for T8157 (\u003Ca href=\u0022https:\/\/www.abmgood.com\/PriGrow-X-Series-Medium-for-T8157-tm8157.html\u0022 target=\u0022_self\u0022\u003ETM8157\u003C\/a\u003E) + 100ng\/ml human Stem Cell Factor (\u003Ca href=\u0022https:\/\/www.abmgood.com\/recombinant-human-stem-cell-factor-kitlg-z100815.html\u0022 target=\u0022_self\u0022\u003EZ100815\u003C\/a\u003E) + 2mM L-Glutamine\u0026nbsp;\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E(\u003C\/span\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\/l-glutamine-g275.html\u0022 target=\u0022_self\u0022 style=\u0022text-wrap: wrap;\u0022\u003EG275\u003C\/a\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E\u003C\/span\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E)\u003C\/span\u003E + 1% Penicillin\/Streptomycin Solution (\u003Ca href=\u0022https:\/\/www.abmgood.com\/penicillin-streptomycin-solution-g255.html\u0022 target=\u0022_self\u0022\u003EG255\u003C\/a\u003E), 37.0\u00b0C, 5% CO\u2082.\u0026nbsp;\u003C\/p\u003E\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EReplace half of the medium with an equal volume of fresh medium, weekly. Do not allow cells to become over-confluent or grow past a density of 1x10\u003C\/span\u003E\u003Csup style=\u0022text-wrap: wrap;\u0022\u003E6\u003C\/sup\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E\u0026nbsp;cells\/ml.\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E\u003Cstrong\u003ENote: it is normal to observe cell death in the first two weeks after thawing. Allow cells to recover under the recommended culture conditons.\u0026nbsp;\u003C\/strong\u003ECells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed.\u0026nbsp;\u003C\/p\u003E\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E*Cells may lose functionality as a result of continuous culture.\u0026nbsp;Degranulation assays must be conducted every 2 months to test functionality -protocol can be found under the \u0026quot;Documents\u0026quot; tab.\u0026nbsp;\u003Cbr\/\u003E\u003C\/p\u003E","recommend":"\u003Cp\u003EWorried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":"\u003Cp\u003EKirshenbaum, A. S., Akin, C., Wu, Y., Rottem, M., Goff, J. P., Beaven, M. A., ... \u0026amp; Metcalfe, D. D. (2003). Characterization of novel stem cell factor responsive human mast cell lines LAD 1 and 2 established from a patient with mast cell sarcoma\/leukemia; activation following aggregation of Fc\u03b5RI or Fc\u03b3RI. Leukemia research, 27(8), 677-682.\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E\u003Cp\u003ES. Kirshenbaum, A., Yin, Y., Sundstrom, J. B., Bandara, G., \u0026amp; D. Metcalfe, D. (2019). Description and characterization of a novel human mast cell line for scientific study. International Journal of Molecular Sciences, 20(22), 5520.\u003C\/p\u003E","cryopreservation":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EFollowing centrifugation, leave a small amount of culturing media (50-100 uL). Suspend cells in non-DMSO, non-FBS containing CryoScarless Cryopreservative (BioVerde) at a final concentration of 5x10\u003C\/span\u003E\u003Csup style=\u0022text-wrap: wrap;\u0022\u003E6\u003C\/sup\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E\u0026nbsp;cells\/mL. Place vials in a cryo container and store at -80\u0026deg;C or in liquid nitrogen for longer preservation.\u003C\/span\u003E\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","promotions":null,"sync_images":null,"thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 850rpm for 5 minutes.\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E\n\u003Cp\u003E6.\u0026nbsp;\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EPost-thaw viability should be 70-95%; however, it is normal to observe increased cell death and debris in the first two-three weeks after the post-thaw period. Remove debris and dead cells during this period by skimming, where possible. Hemidepletion of the media should be performed 1-2 times per week depending on cell density. Allow cells to recover during the first 2-4 weeks post-thaw. In addition, add fresh 100 ng\/ml SCF 1-2 times per week.\u003C\/span\u003E\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2023-05-03 20:38:23","updated_at":"2025-04-04 16:48:00","concentration":null},"seo":{"id":76822,"catalog_id":54479,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"Human-Mast-Cell-Line-LAD2-t8157.html","meta_title":null,"meta_keywords":null,"meta_description":null,"created_at":"2023-05-16 19:03:41","updated_at":"2024-05-13 19:44:57"},"gene":null,"media":[{"id":388214,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/fr5RQt8gaoF0xOkhVlDyKntjIxtvzMiACN30Ao1P.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:04:16","updated_at":"2025-02-13 19:04:16"},{"id":388215,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/I0xsrV7wHTnHYVWqXNr224bu4pC2hSgDngTuIzbF.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:04:25","updated_at":"2025-02-13 19:04:25"},{"id":388216,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/JavR1Fx3SDIGFepMvMY3zUY5rtXxVcqt7LwJeCra.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:04:33","updated_at":"2025-02-13 19:04:33"},{"id":388217,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/CPLL1ZnldJu3MDFvrXjEsGayJldOelmLOL1Vm0zY.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:04:43","updated_at":"2025-02-13 19:04:43"},{"id":388218,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/Kx6FhYQ29aphFRaVk5yGOcrg7czlUnvSLzu9Zctr.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:04:48","updated_at":"2025-02-13 19:04:48"},{"id":382634,"parent_id":54479,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/h5JFbdVBKYKDLqgUh5FniFZnoDibJlzD4ng6g7bq.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2023-06-14 21:31:24","updated_at":"2023-06-14 21:31:24"}]}