Enhanced Primary Human Hepatocytes - 5 million
Cat. No.
T5996
Unit
5x10⁶ cells / 1.0 ml
Price
Inquiry
| Cat. No. | T5996 | ||||||
| Name | Enhanced Primary Human Hepatocytes - 5 million | ||||||
| Description |
Enhanced Primary Human Hepatocytes are expanded primary cells which retains the physiologically relevant profile and phenotype of primary hepatocytes. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for metabolism studies. The late expansion cells are tested for CYP induction and inhibition. It is not recommended for further extensive passaging after thawing but can be used for long term culture which is necessary for cell-based assays.
|
||||||
| Organism | Human (H. sapiens) | ||||||
| Tissue | Liver | ||||||
| Growth Properties | Adherent, polygonal | ||||||
| Cell Type | Primary Cells | ||||||
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. | ||||||
| Shipping Conditions | Ship with dry ice. | ||||||
| Product Format | Frozen | ||||||
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. | ||||||
| BioSafety | II | ||||||
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. | ||||||
| Growth Conditions | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Ready-to-use Hepatocyte Thawing Medium (TM102) and the Enhanced Primary Human Hepatocytes Media Kit (TM103) which comes with the Hepatocyte Growth Basal Medium, and Hepatocyte Growth Supplement Mix are required to grow the cells, 37.0°C, 5% CO₂. | ||||||
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
||||||
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
||||||
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
||||||
| Cryopreservation | Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. | ||||||
| Seeding Density (cells/cm2) | 10,000 | ||||||
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. | ||||||
| Disclaimer |
1. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). 2. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 3. abm warrants that cells shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period". |
||||||
| Application | Research Use Only. | ||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T5996 |
Print/Download Datasheet
Search CoA here
Supporting Protocol
| I want to make sure these cells express my gene of interest before I decide to buy the cell line. Can you provide a sample so this can be tested? | |
|
We do not perform extensive downstream characterization, or gene expression profiling of our cell lines. However, you can check the ‘References’ tab to see if a publication is available, or you can contact us to see if RNA or cell lysates are available for your cell line of interest. Please directly contact us for further information at quotes@abmgood.com.
|
| Does abm's PriGrow series medium contain antibiotics? | |
|
No, the medium does not contain antibiotics and needs to be supplemented by the end-user as desired.
|
| Do I need to use abm's media and Applied Cell Extracellular Matrix (G422) to culture my cells? | |
|
We strongly encourage using the recommended media and culture conditions described in the "Growth Conditions" section as they have been optimized for cell growth. Other supplier's media and coating conditions have not been tested.
|
| What is the difference between Applied Cell Extracellular Matrix (G422) and Collagen Coating? | |
|
The main component of our Applied Cell Extracellular Matrix (G422) is type I collagen specifically. For more information on abm's Applied Cell Extracellular Matrix please visit: http://www.abmgood.com/Applied-Cell-Extracellular-Matrix-G422.html.
|
| Do I have to use T25 ECM-coated flasks for growing the cells? | |
|
We strongly recommend thawing cells in the T25 flasks specified on the cell-specific product page to ensure optimal post-thaw recovery of the frozen cells before testing other culture vessels.
|
| If I want to plate these cells to multi-well plates (e.g. 96 well plates) or dishes, how should I prepare the plates? | |
| What percentage of Trypsin-EDTA should be used to subculture cells? Can I use Trypsin-EDTA containing phenol red? | |
|
The standard concentration used for subculturing is 0.25% Trypsin-EDTA, available at abm (Cat. No. TM050). Yes, Trypsin-EDTA containing phenol red can be used as it does not interfere with trypsinization.
|
| How often do I need to change the media? | |
|
Media should be changed every 2-3 days or as specified within the recommended growth conditions.
|
| Why are these cells classified as biosafety level II? | |
|
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
|
| How long can I store frozen vials? | |
|
Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen vapor phase (or below -130°C) indefinitely without affecting viability.
|
| What is the recommended storage temperature for cells? | |
|
In general, if you received:
|
| Can I substitute heat-inactivated FBS with FBS or vice versa? | |
|
FBS and heat-inactivated FBS are different in their composition; they cannot be substituted for one another.
|
| My cells are not detaching, what method do you recommend to trypsinize the cells? | |
|
| How are live cells shipped? | |
|
Live cells are shipped as a live culture in a T12.5 flask. Cells are seeded at an optimal confluency to avoid metabolic waste build up during transport. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
|
| Why are cells not attaching and forming clumps after subculturing with trypsin? | |
|
Cells form clumps and display attachment issues when the trypsinization agent is not effectively neutralized. To completely neutralize the trypsin agent, add an equal amount of complete growth media prior to centrifugation. Subtle changes in culture conditions, particularly in pH and the quality of serum used in the growth medium, may also affect the amount of clumping exhibited by the cells.
|
| Why are my cells forming clumps after plating? | |
|
It is important to ensure cell clumps are broken up through gentle re-suspension by pipetting up and down several times after pelleting cells by centrifugation. This will ensure a homogenous single cell suspension for even plating.
|
| Do I need to add the selection drug to the complete growth medium? | |
|
We recommend maintaining selection pressure using the drug concentration specified in the Growth Conditions section.
|
| Where can I find the CoA for my product? | |
|
Certificates of analysis can be found here (https://www.abmgood.com/CoA-Library.html).
|
There are no references for this product yet!
This product has no review yet.
Other Cell Lines
SELECT CATEGORY
SPECIES
BIO SYSTEM
CELL TYPE
SEARCH RESULT
{"id":34805,"cat_no":"T5996","retired_cat_no":"","old_cat_no":"T5996","name":"Enhanced Primary Human Hepatocytes - 5 million","price":"0.0000","price_before_discount":"0.0000","academic_price":"0.00","commercial_price":null,"distributor_price_note":null,"unit_quantity":"5x10\u2076 cells \/ 1.0 ml","category_id":72,"category_name":"Primary Cells","frontend_category_id":27,"frontend_category_ids":"86,20,21,27","gene_id":0,"ncbi_id":0,"gene_name":"","gene_full_name":null,"alias":"","accession_number":null,"species_id":6,"species":"Human (H. sapiens)","real_species_id":0,"cell_type_id":2,"cell_type":"Primary Cells","product_type":"Primary","tissue_id":1,"tissue":"Liver","tissue_system":"Digestive System","source":null,"keywords":null,"invisible_search_keywords":null,"filter_group_id":13,"filter_group_name":"Cell Lines","filter_name":"Primary Cells","search_filter_ids":"41,49,102,108","search_filter_keys":"41-49,102-108","temp_field":null,"filter_id":16,"customization":0,"filter_table_id":6091,"filter_table_name":"","related_group_id":8,"operator":null,"status":1,"is_ko":0,"need_remove":0,"units":null,"document_sort":"","price_old":"0.0000","price2024":"0.0000","created_at":null,"updated_at":"2024-12-23 17:54:02","points_rule":5,"Confirmed":"Tony Confirmed","application_no_use":null,"application_id_no_use":null,"raised_in":null,"raised_in_id":null,"geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"Primary-Cells.html","frontendBreadcrumbs":[{"id":20,"pid":86,"name":"Cellular Materials","page_id":18,"page_type":"App\\Models\\Category","href":"","sort":20,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"cellular-materials.html","url":"cellular-materials.html"},{"id":21,"pid":20,"name":"Cell Library Collections","page_id":221,"page_type":"App\\Models\\Category","href":null,"sort":21,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":"2025-02-12 01:11:16","url_key":"cellular-collections.html","url":"cellular-collections.html"},{"id":27,"pid":21,"name":"Primary Cells","page_id":72,"page_type":"App\\Models\\Category","href":null,"sort":27,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"Primary-Cells.html","url":"Primary-Cells.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseCells","info_family":"","geo_price":"Inquiry","geo_price_symbol":"Inquiry","category":{"id":72,"categories":"Primary Cells","pid":18,"url_key":"Primary-Cells.html","css":null,"wid":0,"breadcrumbs":"[{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/\u0022,\u0022title\u0022:\u0022Home\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/cellular-materials.html\u0022,\u0022title\u0022:\u0022Cellular Materials\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/Primary-Cells.html\u0022,\u0022title\u0022:\u0022Primary Cells\u0022}]","breadcrumbsIdJson":"\u003Cscript type=\u0022application\/ld+json\u0022\u003E{\u0022@context\u0022:\u0022https:\/\/schema.org\u0022,\u0022@type\u0022:\u0022BreadcrumbList\u0022,\u0022itemListElement\u0022:[{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:1,\u0022name\u0022:\u0022Home\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\/\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:2,\u0022name\u0022:\u0022Cellular Materials\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\/cellular-materials.html\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:3,\u0022name\u0022:\u0022Primary Cells\u0022}]}\u003C\/script\u003E","breadcrumbsHtml":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\/\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\/cellular-materials.html\u0022\u003ECellular Materials\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022active\u0022\u003EPrimary Cells\u003C\/li\u003E\u003C\/ul\u003E","description":"\u003Ch2 class=\u0022abm-categories-title-h2\u0022\u003EPrimary Cells\u003C\/h2\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cp\u003EPrimary cells are directly isolated from living tissues and retain many of the physiological characteristics of their tissue of origin. Unlike immortalized or transformed cell lines, primary cells offer more biologically relevant models, making them essential for accurate and meaningful life science research.\u003C\/p\u003E\n\u003Ch4 class=\u0022abm-mt-3\u0022\u003E\u003Cstrong\u003EApplications of Primary Cells:\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003EDrug Discovery and Toxicology:\u003C\/strong\u003E Evaluating drug efficacy and toxicity in a natural cellular environment.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ECancer Research:\u003C\/strong\u003E Investigating tumor growth and progression using both normal and cancerous tissue-derived cells.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EImmunology Studies:\u003C\/strong\u003E Exploring immune responses with primary immune cells.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ETissue Engineering:\u003C\/strong\u003E Creating biomimetic tissues for regenerative medicine.\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003ETo support your research needs, \u003Cstrong\u003Eabm\u003C\/strong\u003E offers a collection of over 400 primary cell types from both human and animal origins. Our extensive inventory ensures that you can find the right primary cells for your specific project.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-container-fluid abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022search-box\u0022 id=\u0022search-box\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-row\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-col\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-title\u0022\u003ESELECT CATEGORY\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-line\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-items\u0022\u003E\n\u003Cul\u003E\n\u003Cli class=\u0022search-sub-filter-item-radio\u0022\u003E\u003Clabel class=\u0022abm-radio\u0022\u003E\u003Cinput type=\u0022radio\u0022 name=\u0022search-filter_id\u0022 value=\u002214\u0022 \/\u003E\u003Cspan class=\u0022abm-radio-icon\u0022\u003E\u003C\/span\u003E\u003Cspan class=\u0022abm-radio-text\u0022\u003EImmortalized Cells\u003C\/span\u003E\u003C\/label\u003E\u003C\/li\u003E\n\u003Cli class=\u0022search-sub-filter-item-radio\u0022\u003E\u003Clabel class=\u0022abm-radio\u0022\u003E\u003Cinput type=\u0022radio\u0022 name=\u0022search-filter_id\u0022 value=\u002215\u0022 \/\u003E\u003Cspan class=\u0022abm-radio-icon\u0022\u003E\u003C\/span\u003E\u003Cspan class=\u0022abm-radio-text\u0022\u003ETumor Cells\u003C\/span\u003E\u003C\/label\u003E\u003C\/li\u003E\n\u003Cli class=\u0022search-sub-filter-item-radio\u0022\u003E\u003Clabel class=\u0022abm-radio\u0022\u003E\u003Cinput type=\u0022radio\u0022 name=\u0022search-filter_id\u0022 value=\u002216\u0022 checked=\u0022checked\u0022 \/\u003E\u003Cspan class=\u0022abm-radio-icon\u0022\u003E\u003C\/span\u003E\u003Cspan class=\u0022abm-radio-text\u0022\u003EPrimary Cells\u003C\/span\u003E\u003C\/label\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-col\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-title\u0022\u003ESPECIES\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-line\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-items\u0022 id=\u0022search-sub-filter-organism-box\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-col\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-title\u0022\u003EBIO SYSTEM\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-line\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-items\u0022 id=\u0022search-sub-filter-tissue_system-box\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-col\u0022\u003E\n\u003Cdiv class=\u0022search-sub-filter-title\u0022\u003ECELL TYPE\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-line\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022search-sub-filter-items\u0022 id=\u0022search-sub-filter-tissue-box\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022search-clear\u0022\u003E\u003Cbutton class=\u0022abm-btn\u0022\u003EClear All\u003C\/button\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022result-box\u0022 id=\u0022result-box\u0022\u003E\n\u003Cdiv class=\u0022result-title\u0022\u003ESEARCH RESULT\u003C\/div\u003E\n\u003Cdiv class=\u0022result-line\u0022\u003E\u003C\/div\u003E\n\u003Cdiv id=\u0022result-container\u0022\u003E\u003C\/div\u003E\n\u003Cdiv id=\u0022paginate-container\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cstyle\u003E\n .search-box *,\n .result-box .result-title {\n font-size: 14px;\n }\n\n .search-filter_id,\n .search-sub-filter {\n width: 100%;\n margin-bottom: 25px;\n }\n\n .search-filter_id-title,\n .search-sub-filter-title,\n .result-title {\n background-color: #EF6331;\n color: #fff;\n width: 100%;\n padding: 0 15px;\n overflow: hidden;\n text-overflow: ellipsis;\n word-break: keep-all;\n height: 32px;\n line-height: 32px;\n }\n\n .search-filter_id-line,\n .search-sub-filter-line,\n .result-line {\n width: 100%;\n background-color: #EF6331;\n }\n\n .search-filter_id-items {\n display: flex;\n flex-wrap: wrap;\n border-top: none;\n align-items: center;\n justify-content: space-around;\n padding: 15px 20px;\n }\n\n .search-filter_id-vertical-line {\n width: 1px;\n font-size: 0;\n background-color: #cacaca;\n }\n\n .search-filter_id-items .abm-radio {\n margin: 5px 15px 5px 0;\n }\n\n .search-sub-filter-row {\n display: grid;\n grid-template-columns: 1fr 1fr 1fr 1fr;\n }\n\n .search-sub-filter-col {\n display: flex;\n flex-direction: column;\n }\n\n .search-sub-filter-items {\n flex: 1;\n padding: 15px;\n border-left: 1px solid #ddd;\n border-bottom: 1px solid #ddd;\n }\n\n .search-sub-filter-col:last-child .search-sub-filter-items {\n border-right: 1px solid #ddd;\n }\n\n .search-sub-filter-items ::-webkit-scrollbar {\n width: 10px;\n }\n\n .search-sub-filter-items ::-webkit-scrollbar-track {\n background-color: #eee;\n \/*border-left: 1px solid #ddd;*\/\n \/*border-right: 1px solid #ddd;*\/\n border-radius: 5px;\n }\n\n .search-sub-filter-items ::-webkit-scrollbar-thumb {\n background-color: #ef6331;\n border-radius: 5px;\n }\n\n .search-sub-filter-items ul {\n min-height: 100%;\n height: 200px;\n overflow-x: hidden;\n overflow-y: auto;\n padding: 0;\n margin: -15px;\n }\n\n .search-sub-filter-item,\n .search-sub-filter-item-radio {\n list-style: none;\n padding: 2px 15px;\n cursor: pointer;\n position: relative;\n }\n\n .search-sub-filter-item:hover {\n background-color: #EEE;\n }\n\n .search-sub-filter-item.active,\n .search-sub-filter-item.selected {\n background-color: #CCC;\n }\n\n .search-sub-filter-item-radio label.abm-radio {\n margin-bottom: 0;\n }\n\n .search-sub-filter-item-clear {\n display: none;\n }\n\n .search-sub-filter-item.active .search-sub-filter-item-clear,\n .search-sub-filter-item.selected .search-sub-filter-item-clear {\n display: flex;\n align-items: center;\n position: absolute;\n top: 0;\n right: -1px;\n height: 100%;\n padding: 0 10px;\n background-color: #333;\n color: #fff;\n }\n\n .search-clear {\n text-align: right;\n }\n\n .search-clear button {\n padding: 8px 15px;\n border: 1px solid #EF6331;\n background-color: #EF6331;\n color: #fff;\n border-radius: 4px;\n cursor: pointer;\n opacity: 1;\n transition: .3s;\n }\n\n .search-clear button:not(:disabled):hover {\n opacity: .8;\n }\n\n .search-clear button:disabled {\n opacity: .5;\n cursor: not-allowed;\n }\n\n .result-box {\n margin-top: 50px;\n }\n\n #result-container {\n padding-top: 20px;\n }\n\n #result-container ul {\n list-style: none;\n padding: 0;\n }\n\n .search-result-notice {\n background-color: #f2f2f2;\n padding: 15px 20px;\n margin-bottom: 20px;\n border-radius: 6px;\n }\n\n\n \/* Radio *\/\n .abm-radio {\n display: flex;\n margin-right: 15px;\n align-items: center;\n }\n\n .abm-radio input {\n display: none;\n }\n\n .abm-radio .abm-radio-icon {\n display: block;\n width: 1em;\n height: 1em;\n border-radius: 50%;\n border: 2px solid #ccc;\n margin-right: 5px;\n position: relative;\n }\n\n .abm-radio .abm-radio-text {\n color: #333;\n font-weight: 700;\n }\n\n .abm-radio:hover .abm-radio-icon,\n .abm-radio input:checked+.abm-radio-icon {\n border-color: #EF6331;\n }\n\n .abm-radio:hover .abm-radio-text {\n color: #EF6331;\n }\n\n .abm-radio input:checked+.abm-radio-icon::after {\n content: \u0022\u0022;\n display: block;\n width: 0.5em;\n height: 0.5em;\n background-color: #EF6331;\n border-radius: 50%;\n position: absolute;\n top: 50%;\n left: 50%;\n transform: translate(-50%, -50%);\n }\n\n .abm-radio input:checked+.abm-radio-icon+.abm-radio-text {\n color: #EF6331;\n }\n\n .loading {\n display: flex;\n position: absolute;\n left: 50%;\n top: 50%;\n transform: translate(-50%, -50%);\n }\n\n .loading-item {\n width: 5px;\n height: 20px;\n background-color: #EF6331;\n\n -webkit-animation: loading 1.2s infinite ease-in-out;\n animation: loading 1.2s infinite ease-in-out;\n }\n\n .loading-item+.loading-item {\n margin-left: 5px;\n }\n\n .loading-item:nth-child(2) {\n -webkit-animation-delay: .1s;\n animation-delay: .1s;\n }\n\n .loading-item:nth-child(3) {\n -webkit-animation-delay: .2s;\n animation-delay: .2s;\n }\n\n .loading-item:nth-child(4) {\n -webkit-animation-delay: .3s;\n animation-delay: .3s;\n }\n\n .loading-item:nth-child(5) {\n -webkit-animation-delay: .4s;\n animation-delay: .4s;\n }\n\n @-webkit-keyframes loading {\n\n 0%,\n 40%,\n 100% {\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @keyframes loading {\n\n 0%,\n 40%,\n 100% {\n transform: scaleY(1);\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n transform: scaleY(1.7);\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @media screen and (max-width: 768px) {\n .search-sub-filter-row {\n grid-template-columns: 1fr;\n grid-gap: 15px;\n }\n\n .search-sub-filter-items {\n height: unset;\n border-right: 1px solid #ddd;\n }\n\n .abm-search-item-action {\n display: none;\n }\n }\n\u003C\/style\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const API = \u0027\/product\/searchApi\u0027;\n\n const DEFAULT_FILTER_ID = 16;\n\n function jump(params = {}, id = \u0027search-box\u0027) {\n let url = generateURL(params, location.origin + location.pathname);\n if (id) {\n scrollToID(id);\n }\n history.pushState(params, \u0027\u0027, url);\n search();\n }\n\n function scrollToID(id) {\n const el = document.getElementById(id);\n if (el) {\n window.scrollTo(0, getOffsetTop(el));\n }\n }\n\n function getOffsetTop(el) {\n let result = el.offsetTop;\n if (el.offsetParent) {\n result += getOffsetTop(el.offsetParent);\n }\n return result;\n }\n\n function getParams(state) {\n const params = [];\n for (const key in state) {\n params.push(`${key}=${state[key]}`);\n }\n return params;\n }\n\n function generateURL(state, base = API) {\n const params = getParams(state);\n if (params \u0026\u0026 params.length) {\n base += \u0027?\u0027 + params.join(\u0027\u0026\u0027);\n }\n return base;\n }\n\n function getDefaultFilterId() {\n return getSearchValueByKey(\u0027filter_id\u0027, DEFAULT_FILTER_ID);\n }\n\n function getSearchValueByKey(key, defaultVal = \u0027\u0027) {\n const search = location.search;\n if (search) {\n const str = search.substring(1);\n const arr = str.split(\u0027\u0026\u0027);\n for (const item of arr) {\n const keyValue = item.split(\u0027=\u0027);\n if (keyValue[0] === key) {\n return keyValue[1];\n }\n }\n }\n return defaultVal;\n }\n\n function search() {\n const historyStateExists = !!history.state;\n\n let state = history.state;\n if (!state) {\n state = { filter_id: getDefaultFilterId() };\n } else if (!state.filter_id) {\n state.filter_id = getDefaultFilterId();\n }\n\n for (const item of [\u0027species_id\u0027, \u0027tissue_system\u0027, \u0027tissue_id\u0027, \u0027page\u0027]) {\n const value = getSearchValueByKey(item);\n if (!state[item] \u0026\u0026 value) {\n state[item] = value;\n }\n }\n\n if (!historyStateExists) {\n history.pushState(Object.assign({}, state), \u0027\u0027);\n }\n\n disableFilterSection();\n fetch(generateURL(state)).then(res =\u003E res.json()).then(res =\u003E {\n if (0 === res.code) {\n renderProducts(res.data.products, res.data.page);\n renderSubFilter({\n organism: res.data.species,\n tissue_system: res.data.tissue_system,\n tissue_id: res.data.tissue\n });\n renderPaginate(res.data.page, res.data.lastPage);\n\n const filterId = state.filter_id;\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.checked = el.value + \u0027\u0027 === filterId + \u0027\u0027;\n });\n }\n\n enableFilterSection();\n }).catch(() =\u003E {\n enableFilterSection();\n });\n }\n\n function renderProducts(products, currentPage = 1) {\n let html = \u0027\u0027;\n if (currentPage \u003E 1) {\n html += \u0027\u003Cdiv class=\u0022search-result-notice\u0022\u003EProduct not found? Click \u003Ca href=\u0022javascript:;\u0022 onclick=\u0022scrollToID(\\\u0027search-box\\\u0027)\u0022\u003Ehere\u003C\/a\u003E to refine the result.\u003C\/div\u003E\u0027;\n }\n html += \u0027\u003Cul\u003E\u0027;\n for (const product of products) {\n const url = getProductUrl(product);\n const price = getProductPrice(product);\n\n const mediaPart = generateMediaPart(product);\n const categoryPart = generateCategoryPart(product);\n\n html += `\n \u003Cli class=\u0022abm-search-item\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-container\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-header\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-title\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-name\u0022\u003E\n \u003Ca class=\u0022abm-link\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003E${product.name}\u003C\/a\u003E\n \u003C\/div\u003E\n ${categoryPart}\n \u003C\/div\u003E\n \u003Cdiv class=\u0022abm-search-item-action\u0022\u003E\n \u003Ca class=\u0022btn abm-btn btn-block\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003EView Product\u003C\/a\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003Chr class=\u0022abm-search-item-hr\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-body\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-content\u0022\u003E\n \u003Cul class=\u0022abm-search-item-content-base\u0022\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003ECat. No.:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.cat_no}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EPrice:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${price}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EUnit:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.unit_quantity}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003C\/ul\u003E\n \u003C\/div\u003E\n ${mediaPart}\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n document.querySelector(\u0027#result-container\u0027).innerHTML = html;\n }\n\n function getProductUrl(product) {\n if (product.seo \u0026\u0026 product.seo.url_key) {\n return \u0027\/\u0027 + ltrim(product.seo.url_key, \u0027\/\u0027);\n }\n\n const type = product.model_type.substr(22).toLowerCase();\n return \u0027\/catalog\/products\/\u0027 + product.id + \u0027\/type\/\u0027 + type;\n }\n\n function getProductPrice(product) {\n return isNaN(Number(product.geo_price)) ? (product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? (\u0027\u003Cstrong\u003E\u0027 + item.value + \u0027\u003C\/strong\u003E\u0027) : item.value}\u003C\/span\u003E\n \u003Cdiv class=\u0022search-sub-filter-item-clear\u0022\u003EX\u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n return html;\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-container\u0027).innerHTML = html;\n\n eventListener(\u0027#paginate-container a\u0027, \u0027click\u0027, handlePaginateClick);\n }\n\n function handlePaginateClick() {\n if (this.parentNode.classList.contains(\u0027disabled\u0027)) {\n return;\n }\n const state = history.state || {};\n state.page = this.dataset.page;\n jump(state, \u0027result-box\u0027);\n }\n\n function disableFilterSection() {\n const el = document.querySelector(\u0027.search-box\u0027);\n el.style.position = \u0027relative\u0027;\n addShadow(el, \u0027shadow-search-box\u0027, true);\n\n const elResult = document.querySelector(\u0027.result-box\u0027);\n elResult.style.position = \u0027relative\u0027;\n addShadow(elResult, \u0027shadow-result-box\u0027);\n\n document.querySelector(\u0027.search-clear button\u0027).disabled = false;\n }\n\n function addShadow(elem, id, addLoading = false) {\n const el = document.getElementById(id);\n if (el) {\n el.style.display = \u0027block\u0027;\n el.style.opacity = 1;\n return;\n }\n\n elem.insertAdjacentHTML(\u0027beforeEnd\u0027, genearteShadow(id, addLoading));\n }\n\n function removeShadow(id) {\n const el = document.getElementById(id);\n if (el) {\n el.style.opacity = 0;\n setTimeout(function () {\n el.style.display = \u0027none\u0027;\n }, 500);\n }\n }\n\n function genearteShadow(id, addLoading = false) {\n let html = `\u003Cdiv id=\u0022${id}\u0022 style=\u0022position: absolute; left: 0; top: 0; right: 0; bottom: 0; background-color: rgba(255, 255, 255, .9); z-index: 100; transition: .5s;\u0022\u003E`;\n if (addLoading) {\n html += \u0027\u003Cdiv class=\u0022loading\u0022\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027\n }\n html += `\u003C\/div\u003E`;\n\n return html;\n }\n\n function enableFilterSection() {\n removeShadow(\u0027shadow-search-box\u0027);\n removeShadow(\u0027shadow-result-box\u0027);\n document.querySelector(\u0027.search-clear button\u0027).disabled = !document.querySelector(\u0027.search-sub-filter-item.active\u0027);\n }\n\n search();\n\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.addEventListener(\u0027change\u0027, function () {\n if (el.classList.contains(\u0027prevent\u0027)) {\n return false;\n }\n\n document.querySelector(\u0027input[name=\u0022search-filter_id\u0022][value=\u0022\u0027 + DEFAULT_FILTER_ID + \u0027\u0022]\u0027).checked = true;\n\n switch (el.value + \u0027\u0027) {\n case \u002714\u0027:\n location.href = \u0027\/Immortalized-Cell-Lines.html\u0027;\n break;\n case \u002715\u0027:\n location.href = \u0027\/Tumor-Cell-Lines.html\u0027;\n break;\n case \u002716\u0027:\n location.href = \u0027\/Primary-Cells.html\u0027;\n break;\n default:\n jump({ filter_id: el.value });\n }\n });\n });\n\n document.querySelector(\u0027.search-clear button\u0027).addEventListener(\u0027click\u0027, () =\u003E {\n jump({\n filter_id: getDefaultFilterId()\n });\n });\n\n window.onpopstate = function () {\n \/\/ \u76f4\u63a5\u8fd4\u56de\u4e0a\u4e00\u7ea7\u9875\u9762\n history.back();\n return false;\n if (!history.state) {\n history.back();\n }\n search();\n }\n\u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"Primary Cell Library","meta_keywords":"Primary Cells, human primary cells, animal primary cells, human cell lines, animal cell lines","meta_description":"abm\u2019s comprehensive cell collection provides primary and immortalized cell lines for your research needs. Continuously dividing immortalized cells arise from defined mutational events that allow the primary cells to evade normal cellular senescence. The resulting immortalized primary cells are highly useful for cell biology research as they are significantly easier to culture and maintain compared to their primary counterparts.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":204,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-02-24 00:17:52"},"info":{"id":365,"cat_no_base":"T5996","parent_id":34805,"image":"\/e\/n\/enhanced-primary-human-hepatocytes-cyp-inhibition-data-2.jpg","price-original":null,"description":"\u003Cp\u003E\u003Cp\u003EEnhanced Primary Human Hepatocytes are expanded primary cells which retains the physiologically relevant profile and phenotype of primary hepatocytes. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for metabolism studies. The late expansion cells are tested for CYP induction and inhibition. It is not recommended for further extensive passaging after thawing but can be used for long term culture which is necessary for cell-based assays.\u003C\/p\u003E\u003Cbr\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E\u003Ctable class=\u0022data table additional-attributes product-attribute-specs-table\u0022 width=\u0022NaN\u0022\u003E\u003Ctbody style=\u0022box-sizing: border-box;\u0022\u003E\u003Ctr class=\u0022configProduct firstRow\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003ETo make complete Hepatocyte Growth Basal Medium add the entire content of Hepatocyte Growth Supplement Mix into Hepatocyte Growth Basal Medium and mix properly in BioSafety Cabinet. Addition of the Hepatocyte Growth Supplement Mix may change medium appearance more opaque.\u003Cbr\/\u003E\u003Cbr\/\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003ETo thaw:\u003C\/span\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E1. Pre-warm Hepatocyte Thawing Medium and fully supplemented Hepatocyte Growth Medium to room temperature.\u003Cbr\/\u003E\u003Cbr\/\u003E2. Carefully remove cryovial from storage tank.\u003Cbr\/\u003E\u003Cbr\/\u003E3. Thaw cells in 37\u00b0C water bath until only a small chunk of ice is left.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EDo not shake the vial, or take it out of the water during thawing, as this will damage the cells.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E4. Spray the vial and the tube containing 50 ml of thawing medium with 70% ethanol and transfer to a Biosafety cabinet.\u003Cbr\/\u003E\u003Cbr\/\u003E5. Transfer the now completely thawed cell suspension from the cryovial into 50 ml Hepatocyte Thawing Medium by gently pouring the cells into the medium.\u003Cbr\/\u003E\u003Cbr\/\u003E6. Use a 1 ml pipette to transfer 1 ml of the thawing medium back to the cryovial and pour the contents back into the 50 ml tube. Repeat this process twice to completely remove the cells from the cryovial.\u003Cbr\/\u003E\u003Cbr\/\u003E7. Pellet the cells by centrifuging at 90\u00d7g for 5 min at RT.\u003Cbr\/\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003EImportant note:\u003C\/span\u003E\u0026nbsp;Higher g-forces will significantly reduce cell recovery.\u003Cbr\/\u003E\u003Cbr\/\u003E8. Aspirate the supernatant without disturbing the pellet. Leave approximately 200-400 ?l medium on top of the cells. 9. Gently loosen and re-suspend the cells without adding any extra medium by agitating and rotating the tube.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EDo not vortex or shake the cells as this will compromise cell survival.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E10. Add an appropriate volume of pre-warmed supplemented Hepatocyte Growth Medium to the pellet (~1ml per million cells thawed) and re-suspend the cells.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EAvoid pipetting the cells up and down.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E11. Determine viable cell number by cell count.\u003Cbr\/\u003E\u003Cbr\/\u003E12. Dilute hepatocytes in pre-warmed, fully supplemented Hepatocyte Growth Medium and seed at ~10,000 cells\/cm\u003Cspan style=\u0022box-sizing: border-box; font-size: 11.6071px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;\u0022\u003E2\u003C\/span\u003E\u0026nbsp;in collagen coated cell culture flasks (e.g. T175) or appropriate cell culture dishes.\u003Cbr\/\u003E\u003Cbr\/\u003E13. Incubate the cells at 95% humidity, 37\u00b0C and 5% CO2.\u003Cbr\/\u003E\u003Cbr\/\u003E\u003C\/td\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003E\u003Cbr\/\u003E\u003C\/td\u003E\u003C\/tr\u003E\u003Ctr class=\u0022configProduct\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Cth class=\u0022label\u0022 style=\u0022box-sizing: border-box; text-align: left; border-width: initial; border-style: none; border-color: initial; padding: 4px 30px 25px 0px; vertical-align: top; width: 200px;\u0022\u003ESubculture Protocol\u003C\/th\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003ETo Subculture:\u003C\/span\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E1. Pre-warm PBS, trypsin\/EDTA and Hepatocyte Medium to 37\u003C\/td\u003E\u003C\/tr\u003E\u003Ctr class=\u0022configProduct\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Cth class=\u0022label\u0022 style=\u0022box-sizing: border-box; text-align: left; border-width: initial; border-style: none; border-color: initial; padding: 4px 30px 25px 0px; vertical-align: top; width: 200px;\u0022\u003EPreservation Protocol\u003C\/th\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003EWe do not recommend to freeze down the Enhanced Primary Human Hepatocytes.\u003C\/td\u003E\u003C\/tr\u003E\u003C\/tbody\u003E\u003C\/table\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E","shipment_type":null,"internal_leadtime":"1","lysate_type":null,"small_image":"\/e\/n\/enhanced-primary-human-hepatocytes-cyp-inhibition-data-2.jpg","growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":"Blasticidin resistance marker, Hepatic markers via immunofluorescence staining: CK8+, AAT+, CK18+, HAS+, and AFP- Basal and inducible CYP activities Glycogen storage via PAS staining","storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003E\u0026lt;p\u0026gt;Enhanced Primary Human Hepatocytes are expanded primary cells which retains the physiologically relevant profile and phenotype of primary hepatocytes. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for metabolism studies. The late expansion cells are tested for CYP induction and inhibition. It is not recommended for further extensive passaging after thawing but can be used for long term culture which is necessary for cell-based assays.\u0026lt;\/p\u0026gt;\u0026lt;br\u0026gt;\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E\u003Ctable class=\u0022data table additional-attributes product-attribute-specs-table\u0022 width=\u0022NaN\u0022\u003E\u003Ctbody style=\u0022box-sizing: border-box;\u0022\u003E\u003Ctr class=\u0022configProduct firstRow\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003ETo make complete Hepatocyte Growth Basal Medium add the entire content of Hepatocyte Growth Supplement Mix into Hepatocyte Growth Basal Medium and mix properly in BioSafety Cabinet. Addition of the Hepatocyte Growth Supplement Mix may change medium appearance more opaque.\u003Cbr\/\u003E\u003Cbr\/\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003ETo thaw:\u003C\/span\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E1. Pre-warm Hepatocyte Thawing Medium and fully supplemented Hepatocyte Growth Medium to room temperature.\u003Cbr\/\u003E\u003Cbr\/\u003E2. Carefully remove cryovial from storage tank.\u003Cbr\/\u003E\u003Cbr\/\u003E3. Thaw cells in 37\u00b0C water bath until only a small chunk of ice is left.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EDo not shake the vial, or take it out of the water during thawing, as this will damage the cells.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E4. Spray the vial and the tube containing 50 ml of thawing medium with 70% ethanol and transfer to a Biosafety cabinet.\u003Cbr\/\u003E\u003Cbr\/\u003E5. Transfer the now completely thawed cell suspension from the cryovial into 50 ml Hepatocyte Thawing Medium by gently pouring the cells into the medium.\u003Cbr\/\u003E\u003Cbr\/\u003E6. Use a 1 ml pipette to transfer 1 ml of the thawing medium back to the cryovial and pour the contents back into the 50 ml tube. Repeat this process twice to completely remove the cells from the cryovial.\u003Cbr\/\u003E\u003Cbr\/\u003E7. Pellet the cells by centrifuging at 90\u00d7g for 5 min at RT.\u003Cbr\/\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003EImportant note:\u003C\/span\u003E\u0026nbsp;Higher g-forces will significantly reduce cell recovery.\u003Cbr\/\u003E\u003Cbr\/\u003E8. Aspirate the supernatant without disturbing the pellet. Leave approximately 200-400 ?l medium on top of the cells. 9. Gently loosen and re-suspend the cells without adding any extra medium by agitating and rotating the tube.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EDo not vortex or shake the cells as this will compromise cell survival.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E10. Add an appropriate volume of pre-warmed supplemented Hepatocyte Growth Medium to the pellet (~1ml per million cells thawed) and re-suspend the cells.\u0026nbsp;\u003Cem style=\u0022box-sizing: border-box;\u0022\u003EAvoid pipetting the cells up and down.\u003C\/em\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E11. Determine viable cell number by cell count.\u003Cbr\/\u003E\u003Cbr\/\u003E12. Dilute hepatocytes in pre-warmed, fully supplemented Hepatocyte Growth Medium and seed at ~10,000 cells\/cm\u003Cspan style=\u0022box-sizing: border-box; font-size: 11.6071px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;\u0022\u003E2\u003C\/span\u003E\u0026nbsp;in collagen coated cell culture flasks (e.g. T175) or appropriate cell culture dishes.\u003Cbr\/\u003E\u003Cbr\/\u003E13. Incubate the cells at 95% humidity, 37\u00b0C and 5% CO2.\u003Cbr\/\u003E\u003Cbr\/\u003E\u003C\/td\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003E\u003Cbr\/\u003E\u003C\/td\u003E\u003C\/tr\u003E\u003Ctr class=\u0022configProduct\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Cth class=\u0022label\u0022 style=\u0022box-sizing: border-box; text-align: left; border-width: initial; border-style: none; border-color: initial; padding: 4px 30px 25px 0px; vertical-align: top; width: 200px;\u0022\u003ESubculture Protocol\u003C\/th\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003E\u003Cspan style=\u0022box-sizing: border-box; font-weight: 700;\u0022\u003ETo Subculture:\u003C\/span\u003E\u003Cbr\/\u003E\u003Cbr\/\u003E1. Pre-warm PBS, trypsin\/EDTA and Hepatocyte Medium to 37\u003C\/td\u003E\u003C\/tr\u003E\u003Ctr class=\u0022configProduct\u0022 style=\u0022box-sizing: border-box;\u0022\u003E\u003Cth class=\u0022label\u0022 style=\u0022box-sizing: border-box; text-align: left; border-width: initial; border-style: none; border-color: initial; padding: 4px 30px 25px 0px; vertical-align: top; width: 200px;\u0022\u003EPreservation Protocol\u003C\/th\u003E\u003Ctd class=\u0022col data\u0022 style=\u0022box-sizing: border-box; vertical-align: top; padding: 4px 5px 10px; border-width: initial; border-style: none; border-color: initial;\u0022\u003EWe do not recommend to freeze down the Enhanced Primary Human Hepatocytes.\u003C\/td\u003E\u003C\/tr\u003E\u003C\/tbody\u003E\u003C\/table\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"thawing media is upcyte (MHE001), growth media is upcyte (MHE003)\r\n*G422 coated*","alias":null,"tier_price":null,"operator_no_use":"angela_t","addtime":null,"cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":"Polygonal","expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":"0","cell_morphology_specification":null,"tax_class_id":2,"inventory_location":"TM5996-1 ($85 list price), TM5996-2 + TM5996-3 = ($315 list price), distributors get 20% discount","vector":null,"internal_note":"\u003Cp\u003Eupcyte technologies (CHE002); OEM buy, relabel for sale\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"Research Use Only.","minimal_price":null,"seeding_density":null,"seeding_density_cm":"10,000","seeding_density_ml":null,"source":null,"source_catno":"Cat. CHE002 \u003CBr\u003E$2195 list price, 20% distributor discount to $1","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":null,"source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":"Upcyte","donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":"2","biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"Adherent, polygonal","expression_profile":null,"storage":null,"propagation_method":"\u003Cspan style=\u0022color:#F00;\u0022\u003EUse of Applied Cell Extracellular Matrix (\u003Ca href=\u0022\/Applied-Cell-Extracellular-Matrix-G422.html\u0022 title=\u0022Extracellular Matrix\u0022\u003EG422\u003C\/a\u003E) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.\u003C\/span\u003E \u003Cb\u003EWe strongly recommend end users to purchase the Applied Cell Extracellular Matrix (\u003Ca href=\u0022\/Applied-Cell-Extracellular-Matrix-G422.html\u0022\u003EG422\u003C\/a\u003E) to coat your cell culture vessels.\u003C\/b\u003E Use the ready-to-use Hepatocyte Thawing Medium (\u003Ca href=\u0022\/Hepatocyte-Thawing-Medium-TM102.html\u0022\u003ETM102\u003C\/a\u003E) and the Enhanced Primary Human Hepatocytes Media Kit (\u003Ca href=\u0022\/Hepatocyte-Growth-Medium-Kit-TM103.html\u0022\u003ETM103\u003C\/a\u003E) which comes with the Hepatocyte Growth Basal Medium, and Hepatocyte Growth Supplement Mix available from \u003Cb\u003Eabm \u003C\/b\u003E. Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0\u00b0C. \u003Cbr\u003E\u003Cbr\u003E To make complete Hepatocyte Growth Basal Medium add the entire content of Hepatocyte Growth Supplement Mix into Hepatocyte Growth Basal Medium and mix properly in BioSafety Cabinet. Addition of the Hepatocyte Growth Supplement Mix may change medium appearance more opaque.\n\u003Cbr\u003E\u003Cbr\u003E\n\u003Cb\u003ETo thaw:\u003C\/b\u003E\n\u003Cbr\u003E\u003Cbr\u003E\n1. Pre-warm Hepatocyte Thawing Medium and fully supplemented Hepatocyte Growth Medium to room temperature. \u003Cbr\u003E\u003Cbr\u003E\n2. Carefully remove cryovial from storage tank.\u003Cbr\u003E\u003Cbr\u003E\n3. Thaw cells in 37\u00b0C water bath until only a small chunk of ice is left. \u003Ci\u003EDo not shake the vial, or take it out of the water during thawing, as this will damage the cells.\u003C\/i\u003E\u003Cbr\u003E\u003Cbr\u003E\n4. Spray the vial and the tube containing 50 ml of thawing medium with 70% ethanol and transfer to a Biosafety cabinet.\u003Cbr\u003E\u003Cbr\u003E\n5. Transfer the now completely thawed cell suspension from the cryovial into 50 ml Hepatocyte Thawing Medium by gently pouring the cells into the medium.\u003Cbr\u003E\u003Cbr\u003E\n6. Use a 1 ml pipette to transfer 1 ml of the thawing medium back to the cryovial and pour the contents back into the 50 ml tube. Repeat this process twice to completely remove the cells from the cryovial. \u003Cbr\u003E\u003Cbr\u003E\n7. Pellet the cells by centrifuging at 90\u00d7g for 5 min at RT. \u003Cbr\u003E\u003Cb\u003EImportant note:\u003C\/b\u003E Higher g-forces will significantly\nreduce cell recovery. \u003Cbr\u003E\u003Cbr\u003E\n8. Aspirate the supernatant without disturbing the pellet. Leave approximately 200-400 ?l medium on top of the cells.\n9. Gently loosen and re-suspend the cells without adding any extra medium by agitating and rotating the tube. \u003Ci\u003EDo not vortex or shake the cells as this will compromise cell survival.\u003C\/i\u003E \u003Cbr\u003E\u003Cbr\u003E\n10. Add an appropriate volume of pre-warmed supplemented Hepatocyte Growth Medium to the pellet (~1ml per million cells thawed) and re-suspend the cells. \u003Ci\u003EAvoid pipetting the cells up and down.\u003C\/i\u003E\u003Cbr\u003E\u003Cbr\u003E\n11. Determine viable cell number by cell count.\u003Cbr\u003E\u003Cbr\u003E\n12. Dilute hepatocytes in pre-warmed, fully supplemented Hepatocyte Growth Medium and seed at ~10,000 cells\/cm\u003Csup\u003E2\u003C\/sup\u003E in collagen coated cell culture flasks (e.g. T175) or appropriate cell culture dishes.\u003Cbr\u003E\u003Cbr\u003E\n13. Incubate the cells at 95% humidity, 37\u00b0C and 5% CO2.\n\u003Cbr\u003E\u003Cbr\u003E","usage":null,"freeze_thaw_recovery":null,"str_profile":null,"str_markers":"[]","subculture_protocol":"\u003Cp\u003EVolumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.\u003C\/p\u003E\u003Cp\u003E1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.\u003C\/p\u003E\u003Cp\u003E2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u00b0C, for several minutes to facilitate detachment.\u003C\/p\u003E\u003Cp\u003E3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.\u003C\/p\u003E\u003Cp\u003E4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.\u003C\/p\u003E\u003Cp\u003E5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.\u003C\/p\u003E\u003Cp\u003E6. Incubate the cells at the recommended conditions.\u003C\/p\u003E","preservation_protocol":"We do not recommend to freeze down the Enhanced Primary Human Hepatocytes. ","passage_number":null,"qc":"\u003Cp\u003EEach lot has been tested for:\u003C\/p\u003E\n\u003Col\u003E\n\u003Cli\u003E\u0026gt;90% plateability\u003C\/li\u003E\n\u003Cli\u003EPost-thaw viability\u003C\/li\u003E\n\u003Cli\u003ECMV, HIV, HBV, HCV, and Mycoplasma negative\u003C\/li\u003E\n\u003Cli\u003EHepatic markers via immunofluorescence staining: CK8+, AAT+, CK18+, HAS+, and AFP-\u003C\/li\u003E\n\u003Cli\u003EBasal and inducible CYP activities\u003C\/li\u003E\n\u003Cli\u003EGlycogen storage via PAS staining\u003C\/li\u003E\n\u003C\/ol\u003E","shipping_conditions":"Ship with dry ice.","disclaimer_general":"\u003Cp\u003E1. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic or any other non-RUO application(s).\u003C\/p\u003E\r\n\u003Cp\u003E2. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\r\n\u003Cp\u003E3. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cells shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period\u0022.\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":"Upcyte","internal_product_note":null,"donor_history":null,"split_ratio":null,"expression":null,"growth_conditions":"Use of PriCoat\u2122 T25 Flasks (\u003Ca class=\u0022abm-link\u0022 href=\u0022\/pricoat-t25-flasks.html\u0022\u003EG299\u003C\/a\u003E) or Applied Cell Extracellular Matrix (\u003Ca class=\u0022abm-link\u0022 href=\u0022\/applied-cell-extracellular-matrix-g422.html \u0022\u003EG422\u003C\/a\u003E) is required for cell adhesion to the culture vessels. Ready-to-use Hepatocyte Thawing Medium (TM102) and the Enhanced Primary Human Hepatocytes Media Kit (TM103) which comes with the Hepatocyte Growth Basal Medium, and Hepatocyte Growth Supplement Mix are required to grow the cells, 37.0\u00b0C, 5% CO\u2082.","recommend":"","references":null,"cryopreservation":"Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a \r\ntemperature below -130\u00b0C, preferably in liquid nitrogen vapor phase \r\nstorage, until ready for use.\u003C\/p\u003E\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate \r\nculture as soon as possible upon receipt. If continued storage is \r\ndesired, the vial should only be stored below -130\u00b0C or in liquid \r\nnitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss \r\nof viability.\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E","promotions":null,"sync_images":"https:\/\/admin.abmgoodchina.com\/uploads\/images\/cells\/T5996_Morphology.jpg,https:\/\/admin.abmgoodchina.com\/website_img\/e\/n\/enhanced-primary-human-hepatocytes-cyp-data.jpg,https:\/\/admin.abmgoodchina.com\/website_img\/e\/n\/enhanced-primary-human-hepatocytes-cyp-inhibition-data-2.jpg,https:\/\/admin.abmgoodchina.com\/website_img\/e\/n\/enhanced-primary-human-hepatocytes-photomicrographs-2.jpg,https:\/\/admin.abmgoodchina.com\/website_img\/t\/5\/t5996-enhanced-primary-human-hepatocytes-cells.jpg","thawing_protocol":"\u003Cp\u003E\r\n 1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently \r\n(maximum 2 minutes). The vial cap should be kept above the water level \r\nto minimize the risk of contamination.\r\n\u003C\/p\u003E\r\n\u003Cp\u003E\r\n 2. Decontaminate the vial by spraying and wiping the exterior of the \r\nvial with 70% ethanol. From this point onwards, all operations should be\r\n strictly carried out inside a biological safety cabinet using aseptic \r\nconditions.\r\n\u003C\/p\u003E\r\n\u003Cp\u003E\r\n 3. Transfer the cell suspension into a 15ml sterile conical tube \r\ncontaining 5ml of pre-warmed, complete growth media. Centrifuge cells at\r\n 125xg for 5-7 minutes.\r\n\u003C\/p\u003E\r\n\u003Cp\u003E\r\n 4. Aspirate the supernatant without disturbing the cell pellet. \r\nRe-suspend the cell pellet in the recommended pre-warmed, complete \r\ngrowth media and dispense into a T25 culture flask.\r\n\u003C\/p\u003E\r\n\u003Cp\u003E\r\n 5. Incubate the cells at the recommended conditions.\r\n\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-07 08:13:18","updated_at":"2025-03-03 21:07:26","concentration":null},"seo":{"id":67515,"catalog_id":34805,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"enhanced-primary-human-hepatocytes-5-million-t5996.html","meta_title":null,"meta_keywords":null,"meta_description":null,"created_at":"2022-09-23 06:53:13","updated_at":"2024-05-13 19:44:57"},"gene":null,"media":[{"id":382821,"parent_id":34805,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/images\/cells\/T5996_Morphology.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-03-01 05:55:50","updated_at":"2024-03-01 05:55:50"},{"id":130216,"parent_id":34805,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/t\/5\/t5996-enhanced-primary-human-hepatocytes-cells.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":17187599,"sku_in_m2":"T5996","value_id_m2":48049062,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":130217,"parent_id":34805,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/e\/n\/enhanced-primary-human-hepatocytes-photomicrographs-2.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":17187599,"sku_in_m2":"T5996","value_id_m2":48049063,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":130218,"parent_id":34805,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/e\/n\/enhanced-primary-human-hepatocytes-cyp-data.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":0,"entity_id_m2":17187599,"sku_in_m2":"T5996","value_id_m2":48049064,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":130219,"parent_id":34805,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/e\/n\/enhanced-primary-human-hepatocytes-cyp-inhibition-data-2.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":4,"status":0,"entity_id_m2":17187599,"sku_in_m2":"T5996","value_id_m2":48049065,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"}]}