Immortalized Human Hair Follicle Inner Root Sheath Cell Line - SV40 + hTERT
Cat. No.
T9233
Unit
1x106 cells / 1.0 ml
Price
Inquiry
Worried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under abm's Cell Line Insurance.
Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.
For for-profit organizations, please contact quotes@abmgood.com for pricing.
| Cat. No. | T9233 |
| Name | Immortalized Human Hair Follicle Inner Root Sheath Cell Line - SV40 + hTERT |
| Organism | Human (H. sapiens) |
| Tissue | Hair Follicle |
| Growth Properties | Adherent, polygonal |
| Cell Type | Immortalized Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂ |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
| Cryopreservation |
Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Seeding Density (cells/cm2) | 30,000 |
| Population Doubling Time (h) | 24 - 48 |
| Immortalization Method |
Serial passaging and infection with lentivirus carrying SV40 and hTERT genes |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period." |
| Application | Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T9233 |
Print/Download Datasheet
| I want to make sure these cells express my gene of interest before I decide to buy the cell line. Can you provide a sample so this can be tested? | |
|
We do not perform extensive downstream characterization, or gene expression profiling of our cell lines. However, you can check the ‘References’ tab to see if a publication is available, or you can contact us to see if RNA or cell lysates are available for your cell line of interest. Please directly contact us for further information at quotes@abmgood.com.
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| Does abm's PriGrow series medium contain antibiotics? | |
|
No, the medium does not contain antibiotics and needs to be supplemented by the end-user as desired.
|
| Do I need to use abm's media and Applied Cell Extracellular Matrix (G422) to culture my cells? | |
|
We strongly encourage using the recommended media and culture conditions described in the "Growth Conditions" section as they have been optimized for cell growth. Other supplier's media and coating conditions have not been tested.
|
| What is the difference between Applied Cell Extracellular Matrix (G422) and Collagen Coating? | |
|
The main component of our Applied Cell Extracellular Matrix (G422) is type I collagen specifically. For more information on abm's Applied Cell Extracellular Matrix please visit: http://www.abmgood.com/Applied-Cell-Extracellular-Matrix-G422.html.
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| Do I have to use T25 ECM-coated flasks for growing the cells? | |
|
We strongly recommend thawing cells in the T25 flasks specified on the cell-specific product page to ensure optimal post-thaw recovery of the frozen cells before testing other culture vessels.
|
| If I want to plate these cells to multi-well plates (e.g. 96 well plates) or dishes, how should I prepare the plates? | |
| What percentage of Trypsin-EDTA should be used to subculture cells? Can I use Trypsin-EDTA containing phenol red? | |
|
The standard concentration used for subculturing is 0.25% Trypsin-EDTA, available at abm (Cat. No. TM050). Yes, Trypsin-EDTA containing phenol red can be used as it does not interfere with trypsinization.
|
| How often do I need to change the media? | |
|
Media should be changed every 2-3 days or as specified within the recommended growth conditions.
|
| Why are these cells classified as biosafety level II? | |
|
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
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| How long can I store frozen vials? | |
|
Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen vapor phase (or below -130°C) indefinitely without affecting viability.
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| What is the recommended storage temperature for cells? | |
|
In general, if you received:
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| Can I substitute heat-inactivated FBS with FBS or vice versa? | |
|
FBS and heat-inactivated FBS are different in their composition; they cannot be substituted for one another.
|
| My cells are not detaching, what method do you recommend to trypsinize the cells? | |
|
| How are live cells shipped? | |
|
Live cells are shipped as a live culture in a T12.5 flask. Cells are seeded at an optimal confluency to avoid metabolic waste build up during transport. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
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| Why are cells not attaching and forming clumps after subculturing with trypsin? | |
|
Cells form clumps and display attachment issues when the trypsinization agent is not effectively neutralized. To completely neutralize the trypsin agent, add an equal amount of complete growth media prior to centrifugation. Subtle changes in culture conditions, particularly in pH and the quality of serum used in the growth medium, may also affect the amount of clumping exhibited by the cells.
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| Why are my cells forming clumps after plating? | |
|
It is important to ensure cell clumps are broken up through gentle re-suspension by pipetting up and down several times after pelleting cells by centrifugation. This will ensure a homogenous single cell suspension for even plating.
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| Do I need to add the selection drug to the complete growth medium? | |
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We recommend maintaining selection pressure using the drug concentration specified in the Growth Conditions section.
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| Where can I find the CoA for my product? | |
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Certificates of analysis can be found here (https://www.abmgood.com/CoA-Library.html).
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There are no references for this product yet!
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margin-right: 15px;\n align-items: center;\n }\n\n .abm-radio input {\n display: none;\n }\n\n .abm-radio .abm-radio-icon {\n display: block;\n width: 1em;\n height: 1em;\n border-radius: 50%;\n border: 2px solid #ccc;\n margin-right: 5px;\n position: relative;\n }\n\n .abm-radio .abm-radio-text {\n color: #333;\n font-weight: 700;\n }\n\n .abm-radio:hover .abm-radio-icon,\n .abm-radio input:checked+.abm-radio-icon {\n border-color: #EF6331;\n }\n\n .abm-radio:hover .abm-radio-text {\n color: #EF6331;\n }\n\n .abm-radio input:checked+.abm-radio-icon::after {\n content: \u0022\u0022;\n display: block;\n width: 0.5em;\n height: 0.5em;\n background-color: #EF6331;\n border-radius: 50%;\n position: absolute;\n top: 50%;\n left: 50%;\n transform: translate(-50%, -50%);\n }\n\n .abm-radio input:checked+.abm-radio-icon+.abm-radio-text {\n color: #EF6331;\n }\n\n .loading {\n display: flex;\n position: absolute;\n left: 50%;\n top: 50%;\n transform: translate(-50%, -50%);\n }\n\n .loading-item {\n width: 5px;\n height: 20px;\n background-color: #EF6331;\n\n -webkit-animation: loading 1.2s infinite ease-in-out;\n animation: loading 1.2s infinite ease-in-out;\n }\n\n .loading-item+.loading-item {\n margin-left: 5px;\n }\n\n .loading-item:nth-child(2) {\n -webkit-animation-delay: .1s;\n animation-delay: .1s;\n }\n\n .loading-item:nth-child(3) {\n -webkit-animation-delay: .2s;\n animation-delay: .2s;\n }\n\n .loading-item:nth-child(4) {\n -webkit-animation-delay: .3s;\n animation-delay: .3s;\n }\n\n .loading-item:nth-child(5) {\n -webkit-animation-delay: .4s;\n animation-delay: .4s;\n }\n\n .abm-image-zoomable {\n cursor: zoom-in;\n }\n\n @-webkit-keyframes loading {\n\n 0%,\n 40%,\n 100% {\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @keyframes loading {\n\n 0%,\n 40%,\n 100% {\n transform: scaleY(1);\n -webkit-transform: scaleY(1);\n }\n\n 20% {\n transform: scaleY(1.7);\n -webkit-transform: scaleY(1.7);\n }\n }\n\n @media screen and (max-width: 768px) {\n .search-sub-filter-row {\n grid-template-columns: 1fr;\n grid-gap: 15px;\n }\n\n .search-sub-filter-items {\n height: unset;\n border-right: 1px solid #ddd;\n }\n\n .abm-search-item-action {\n display: none;\n }\n }\n\u003C\/style\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const API = \u0027\/product\/searchApi\u0027;\n\n const DEFAULT_FILTER_ID = 14;\n\n function jump(params = {}, id = \u0027search-box\u0027) {\n let url = generateURL(params, location.origin + location.pathname);\n if (id) {\n scrollToID(id);\n }\n history.pushState(params, \u0027\u0027, url);\n search();\n }\n\n function scrollToID(id) {\n const el = document.getElementById(id);\n if (el) {\n window.scrollTo(0, getOffsetTop(el));\n }\n }\n\n function getOffsetTop(el) {\n let result = el.offsetTop;\n if (el.offsetParent) {\n result += getOffsetTop(el.offsetParent);\n }\n return result;\n }\n\n function getParams(state) {\n const params = [];\n for (const key in state) {\n params.push(`${key}=${state[key]}`);\n }\n return params;\n }\n\n function generateURL(state, base = API) {\n const params = getParams(state);\n if (params \u0026\u0026 params.length) {\n base += \u0027?\u0027 + params.join(\u0027\u0026\u0027);\n }\n return base;\n }\n\n function getDefaultFilterId() {\n return getSearchValueByKey(\u0027filter_id\u0027, DEFAULT_FILTER_ID);\n }\n\n function getSearchValueByKey(key, defaultVal = \u0027\u0027) {\n const search = location.search;\n if (search) {\n const str = search.substring(1);\n const arr = str.split(\u0027\u0026\u0027);\n for (const item of arr) {\n const keyValue = item.split(\u0027=\u0027);\n if (keyValue[0] === key) {\n return keyValue[1];\n }\n }\n }\n return defaultVal;\n }\n\n function search() {\n const historyStateExists = !!history.state;\n\n let state = history.state;\n if (!state) {\n state = { filter_id: getDefaultFilterId() };\n } else if (!state.filter_id) {\n state.filter_id = getDefaultFilterId();\n }\n\n for (const item of [\u0027species_id\u0027, \u0027tissue_system\u0027, \u0027tissue_id\u0027, \u0027page\u0027]) {\n const value = getSearchValueByKey(item);\n if (!state[item] \u0026\u0026 value) {\n state[item] = value;\n }\n }\n\n if (!historyStateExists) {\n history.pushState(Object.assign({}, state), \u0027\u0027);\n }\n\n disableFilterSection();\n fetch(generateURL(state)).then(res =\u003E res.json()).then(res =\u003E {\n if (0 === res.code) {\n renderProducts(res.data.products, res.data.page);\n renderSubFilter({\n organism: res.data.species,\n tissue_system: res.data.tissue_system,\n tissue_id: res.data.tissue\n });\n renderPaginate(res.data.page, res.data.lastPage);\n\n const filterId = state.filter_id;\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.checked = el.value + \u0027\u0027 === filterId + \u0027\u0027;\n });\n }\n\n enableFilterSection();\n }).catch(() =\u003E {\n enableFilterSection();\n });\n }\n\n function renderProducts(products, currentPage = 1) {\n let html = \u0027\u0027;\n if (currentPage \u003E 1) {\n html += \u0027\u003Cdiv class=\u0022search-result-notice\u0022\u003EProduct not found? Click \u003Ca href=\u0022javascript:;\u0022 onclick=\u0022scrollToID(\\\u0027search-box\\\u0027)\u0022\u003Ehere\u003C\/a\u003E to refine the result.\u003C\/div\u003E\u0027;\n }\n html += \u0027\u003Cul\u003E\u0027;\n for (const product of products) {\n const url = getProductUrl(product);\n const price = getProductPrice(product);\n\n const mediaPart = generateMediaPart(product);\n const categoryPart = generateCategoryPart(product);\n\n html += `\n \u003Cli class=\u0022abm-search-item\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-container\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-header\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-title\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-name\u0022\u003E\n \u003Ca class=\u0022abm-link\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003E${product.name}\u003C\/a\u003E\n \u003C\/div\u003E\n ${categoryPart}\n \u003C\/div\u003E\n \u003Cdiv class=\u0022abm-search-item-action\u0022\u003E\n \u003Ca class=\u0022btn abm-btn btn-block\u0022 href=\u0022${url}\u0022 title=\u0022${product.name}\u0022\u003EView Product\u003C\/a\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003Chr class=\u0022abm-search-item-hr\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-body\u0022\u003E\n \u003Cdiv class=\u0022abm-search-item-content\u0022\u003E\n \u003Cul class=\u0022abm-search-item-content-base\u0022\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003ECat. No.:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.cat_no}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EPrice:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${price}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EUnit:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.unit_quantity}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003C\/ul\u003E\n \u003C\/div\u003E\n ${mediaPart}\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n document.querySelector(\u0027#result-container\u0027).innerHTML = html;\n }\n\n function getProductUrl(product) {\n if (product.seo \u0026\u0026 product.seo.url_key) {\n return \u0027\/\u0027 + ltrim(product.seo.url_key, \u0027\/\u0027);\n }\n\n const type = product.model_type.substr(22).toLowerCase();\n return \u0027\/catalog\/products\/\u0027 + product.id + \u0027\/type\/\u0027 + type;\n }\n\n function getProductPrice(product) {\n return isNaN(Number(product.geo_price)) ? (product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media abm-image-zoomable\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? (\u0027\u003Cstrong\u003E\u0027 + item.value + \u0027\u003C\/strong\u003E\u0027) : item.value}\u003C\/span\u003E\n \u003Cdiv class=\u0022search-sub-filter-item-clear\u0022\u003EX\u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n return html;\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-container\u0027).innerHTML = html;\n\n eventListener(\u0027#paginate-container a\u0027, \u0027click\u0027, handlePaginateClick);\n }\n\n function handlePaginateClick() {\n if (this.parentNode.classList.contains(\u0027disabled\u0027)) {\n return;\n }\n const state = history.state || {};\n state.page = this.dataset.page;\n jump(state, \u0027result-box\u0027);\n }\n\n function disableFilterSection() {\n const el = document.querySelector(\u0027.search-box\u0027);\n el.style.position = \u0027relative\u0027;\n addShadow(el, \u0027shadow-search-box\u0027, true);\n\n const elResult = document.querySelector(\u0027.result-box\u0027);\n elResult.style.position = \u0027relative\u0027;\n addShadow(elResult, \u0027shadow-result-box\u0027);\n\n document.querySelector(\u0027.search-clear button\u0027).disabled = false;\n }\n\n function addShadow(elem, id, addLoading = false) {\n const el = document.getElementById(id);\n if (el) {\n el.style.display = \u0027block\u0027;\n el.style.opacity = 1;\n return;\n }\n\n elem.insertAdjacentHTML(\u0027beforeEnd\u0027, genearteShadow(id, addLoading));\n }\n\n function removeShadow(id) {\n const el = document.getElementById(id);\n if (el) {\n el.style.opacity = 0;\n setTimeout(function () {\n el.style.display = \u0027none\u0027;\n }, 500);\n }\n }\n\n function genearteShadow(id, addLoading = false) {\n let html = `\u003Cdiv id=\u0022${id}\u0022 style=\u0022position: absolute; left: 0; top: 0; right: 0; bottom: 0; background-color: rgba(255, 255, 255, .9); z-index: 100; transition: .5s;\u0022\u003E`;\n if (addLoading) {\n html += \u0027\u003Cdiv class=\u0022loading\u0022\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027\n }\n html += `\u003C\/div\u003E`;\n\n return html;\n }\n\n function enableFilterSection() {\n removeShadow(\u0027shadow-search-box\u0027);\n removeShadow(\u0027shadow-result-box\u0027);\n document.querySelector(\u0027.search-clear button\u0027).disabled = !document.querySelector(\u0027.search-sub-filter-item.active\u0027);\n }\n\n search();\n\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.addEventListener(\u0027change\u0027, function () {\n if (el.classList.contains(\u0027prevent\u0027)) {\n return false;\n }\n\n document.querySelector(\u0027input[name=\u0022search-filter_id\u0022][value=\u0022\u0027 + DEFAULT_FILTER_ID + \u0027\u0022]\u0027).checked = true;\n\n switch (el.value + \u0027\u0027) {\n case \u002714\u0027:\n location.href = \u0027\/Immortalized-Cell-Lines.html\u0027;\n break;\n case \u002715\u0027:\n location.href = \u0027\/Tumor-Cell-Lines.html\u0027;\n break;\n case \u002716\u0027:\n location.href = \u0027\/Primary-Cells.html\u0027;\n break;\n default:\n jump({ filter_id: el.value });\n }\n });\n });\n\n document.querySelector(\u0027.search-clear button\u0027).addEventListener(\u0027click\u0027, () =\u003E {\n jump({\n filter_id: getDefaultFilterId()\n });\n });\n\n \/\/----------------\n \/\/ \u56fe\u7247\u653e\u5927 START\n \/\/----------------\n document.addEventListener(\u0027click\u0027, (e) =\u003E {\n const elements = document.querySelectorAll(\u0027.abm-image-zoomable\u0027);\n let curr = null;\n for (const element of elements) {\n if (element.contains(e.target)) {\n curr = element;\n break;\n }\n }\n\n if (!curr) {\n return false;\n }\n\n abmImageZoom(curr);\n });\n\n function abmImageZoom(el) {\n const images = el.querySelectorAll(\u0027img\u0027);\n const id = \u0027abm-image-zoom-carousel-\u0027 + parseInt(new Date().getTime());\n let items = \u0027\u0027;\n let hasActive = false;\n let active = null;\n for (const image of images) {\n const parent = image.parentElement;\n if (parent \u0026\u0026 parent.classList.contains(\u0027active\u0027)) {\n active = image;\n break;\n }\n }\n\n for (const image of images) {\n let activeClass = \u0027\u0027;\n if (active) {\n activeClass = active === image ? 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Please verify that the product \nis suitable for your studies by referencing published papers or ordering\n RNA (0.5 \u03bcg, Cat.# C207, $450.00) or cell lysate (100 \u03bcg, Cat.# C206, \n$600.00) to perform preliminary experiments, or alternatively use our \nGene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this \noption can be requested at the time of ordering. Please note that the \nend-user will need to evaluate the feasibility of live cell shipment by \ntaking into account the final destination\u0026#39;s temperature variation and \nits geographical location. In addition, we thoroughly test our cell \nlines for freeze-thaw recovery. If frozen cells were received and not \nrecovered in your lab under the exact, specified conditions (using \nrecommended culture vessel, media, additional supplements, and \natmospheric conditions), a live cell replacement is possible at a cost \n(plus shipping).\u003C\/p\u003E\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026#39;s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E\n is not liable for any repercussions arising from the use of its cell \nbiology product(s) in therapeutic\/diagnostic application(s). Please \ncontact a technical service representative for more information.\u003C\/p\u003E\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to \nthe accuracy of the information on this site. Citations from literature \nand provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon \ninitiation of culture for a period of thirty (30) days after shipment \nand that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E\n Material Product Information sheet, certificate of analysis, and\/or \ncatalog description. 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Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":null,"cryopreservation":"\u003Cp\u003ECryopreservation Medium (TM024), or complete growth media with 10% DMSO.\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a \ntemperature below -130\u00b0C, preferably in liquid nitrogen vapor phase \nstorage, until ready for use.\u003C\/p\u003E\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate \nculture as soon as possible upon receipt. If continued storage is \ndesired, the vial should only be stored below -130\u00b0C or in liquid \nnitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss \nof viability.\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E","promotions":null,"sync_images":null,"thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently \n(maximum 2 minutes). The vial cap should be kept above the water level \nto minimize the risk of contamination.\u003C\/p\u003E\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the \nvial with 70% ethanol. From this point onwards, all operations should be\n strictly carried out inside a biological safety cabinet using aseptic \nconditions.\u003C\/p\u003E\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube \ncontaining 5ml of pre-warmed, complete growth media. Centrifuge cells at\n 125xg for 5-7 minutes.\u003C\/p\u003E\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. \nRe-suspend the cell pellet in the recommended pre-warmed, complete \ngrowth media and dispense into a T25 culture flask.\u003C\/p\u003E\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-07 08:13:18","updated_at":"2025-02-06 17:45:11","concentration":null},"seo":{"id":68188,"catalog_id":37021,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"immortalized-human-hair-follicle-inner-root-sheath-cells-sv40-htert.html","meta_title":null,"meta_keywords":null,"meta_description":null,"created_at":"2022-09-23 06:53:13","updated_at":"2024-05-13 19:44:57"},"gene":null,"media":[{"id":385637,"parent_id":37021,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/GMrQjTUcfOMeAfTbfzegBzVeSPyiEZ8fiOD6Spt3.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-01-16 21:21:47","updated_at":"2025-01-16 21:21:47"}]}