Immortalized Mast Cells (NCL-2) ; Capable of histamine release (degranulation)

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Examples include:\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003ESkin cells for the beauty industry\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/mast-cell-lines.html\u0022 class=\u0022abm-text-bold abm-link\u0022\u003EMast cells\u003C\/a\u003E for allergy research\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/dermal-papilla-cells.html\u0022 class=\u0022abm-text-bold abm-link\u0022\u003EDermal papilla cell lines\u003C\/a\u003E from androgenetic alopecia for hair growth research and drug development\u003C\/li\u003E\n\u003Cli\u003ECow and chicken muscle cells for in \u003Ci\u003Evitro\u003C\/i\u003E meat production\u003C\/li\u003E\n\u003Cli\u003EImmortalized human hepatocytes for toxicity studies in new drug development\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003EIn addition to our extensive catalog, we also offer custom cell immortalization services to meet your specific needs. 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(product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media abm-image-zoomable\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? (\u0027\u003Cstrong\u003E\u0027 + item.value + \u0027\u003C\/strong\u003E\u0027) : item.value}\u003C\/span\u003E\n \u003Cdiv class=\u0022search-sub-filter-item-clear\u0022\u003EX\u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n return html;\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-container\u0027).innerHTML = html;\n\n eventListener(\u0027#paginate-container a\u0027, \u0027click\u0027, handlePaginateClick);\n }\n\n function handlePaginateClick() {\n if (this.parentNode.classList.contains(\u0027disabled\u0027)) {\n return;\n }\n const state = history.state || {};\n state.page = this.dataset.page;\n jump(state, \u0027result-box\u0027);\n }\n\n function disableFilterSection() {\n const el = document.querySelector(\u0027.search-box\u0027);\n el.style.position = \u0027relative\u0027;\n addShadow(el, \u0027shadow-search-box\u0027, true);\n\n const elResult = document.querySelector(\u0027.result-box\u0027);\n elResult.style.position = \u0027relative\u0027;\n addShadow(elResult, \u0027shadow-result-box\u0027);\n\n document.querySelector(\u0027.search-clear button\u0027).disabled = false;\n }\n\n function addShadow(elem, id, addLoading = false) {\n const el = document.getElementById(id);\n if (el) {\n el.style.display = \u0027block\u0027;\n el.style.opacity = 1;\n return;\n }\n\n elem.insertAdjacentHTML(\u0027beforeEnd\u0027, genearteShadow(id, addLoading));\n }\n\n function removeShadow(id) {\n const el = document.getElementById(id);\n if (el) {\n el.style.opacity = 0;\n setTimeout(function () {\n el.style.display = \u0027none\u0027;\n }, 500);\n }\n }\n\n function genearteShadow(id, addLoading = false) {\n let html = `\u003Cdiv id=\u0022${id}\u0022 style=\u0022position: absolute; left: 0; top: 0; right: 0; bottom: 0; background-color: rgba(255, 255, 255, .9); z-index: 100; transition: .5s;\u0022\u003E`;\n if (addLoading) {\n html += \u0027\u003Cdiv class=\u0022loading\u0022\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027\n }\n html += `\u003C\/div\u003E`;\n\n return html;\n }\n\n function enableFilterSection() {\n removeShadow(\u0027shadow-search-box\u0027);\n removeShadow(\u0027shadow-result-box\u0027);\n document.querySelector(\u0027.search-clear button\u0027).disabled = !document.querySelector(\u0027.search-sub-filter-item.active\u0027);\n }\n\n search();\n\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.addEventListener(\u0027change\u0027, function () {\n if (el.classList.contains(\u0027prevent\u0027)) {\n return false;\n }\n\n document.querySelector(\u0027input[name=\u0022search-filter_id\u0022][value=\u0022\u0027 + DEFAULT_FILTER_ID + \u0027\u0022]\u0027).checked = true;\n\n switch (el.value + \u0027\u0027) {\n case \u002714\u0027:\n location.href = \u0027\/Immortalized-Cell-Lines.html\u0027;\n break;\n case \u002715\u0027:\n location.href = \u0027\/Tumor-Cell-Lines.html\u0027;\n break;\n case \u002716\u0027:\n location.href = \u0027\/Primary-Cells.html\u0027;\n break;\n default:\n jump({ filter_id: el.value });\n }\n });\n });\n\n document.querySelector(\u0027.search-clear button\u0027).addEventListener(\u0027click\u0027, () =\u003E {\n jump({\n filter_id: getDefaultFilterId()\n });\n });\n\n \/\/----------------\n \/\/ \u56fe\u7247\u653e\u5927 START\n \/\/----------------\n document.addEventListener(\u0027click\u0027, (e) =\u003E {\n const elements = document.querySelectorAll(\u0027.abm-image-zoomable\u0027);\n let curr = null;\n for (const element of elements) {\n if (element.contains(e.target)) {\n curr = element;\n break;\n }\n }\n\n if (!curr) {\n return false;\n }\n\n abmImageZoom(curr);\n });\n\n function abmImageZoom(el) {\n const images = el.querySelectorAll(\u0027img\u0027);\n const id = \u0027abm-image-zoom-carousel-\u0027 + parseInt(new Date().getTime());\n let items = \u0027\u0027;\n let hasActive = false;\n let active = null;\n for (const image of images) {\n const parent = image.parentElement;\n if (parent \u0026\u0026 parent.classList.contains(\u0027active\u0027)) {\n active = image;\n break;\n }\n }\n\n for (const image of images) {\n let activeClass = \u0027\u0027;\n if (active) {\n activeClass = active === image ? \u0027active\u0027 : \u0027\u0027;\n } else {\n activeClass = hasActive ? \u0027\u0027 : \u0027active\u0027;\n }\n items += `\u003Cdiv class=\u0022item ${activeClass}\u0022\u003E\u003Cimg src=\u0022${image.src}\u0022\u003E\u003C\/div\u003E`;\n hasActive = true;\n }\n if (!items) {\n return false;\n }\n items += `\u003Cstyle\u003E#abm-image-zoomable-modal .modal-dialog { max-width: 1200px; }\n #${id} .carousel-inner .item { position: relative; width: 100%; padding-top: 66.67%; overflow: hidden; }\n #${id} .carousel-inner .item.active.left, #${id} .carousel-inner .item.active.right { position: absolute; top: 0; }\n #${id} .carousel-inner .item img { position: absolute; left: 50%; top: 50%; transform: translate(-50%, -50%); max-width: 100%; max-height: 100%; }\n #${id} .carousel-control { display: none; color: #EF6331; }`;\n if (images.length \u003E 1) {\n items += ` #${id}:hover .carousel-control { display: block; }`;\n }\n items += \u0027\u003C\/style\u003E\u0027;\n\n const zoomModalEl = document.createElement(\u0027div\u0027);\n zoomModalEl.id = \u0027abm-image-zoomable-modal\u0027;\n zoomModalEl.classList.add(\u0027modal\u0027, \u0027fade\u0027);\n zoomModalEl.setAttribute(\u0027tabindex\u0027, \u0027-1\u0027);\n zoomModalEl.setAttribute(\u0027role\u0027, \u0027dialog\u0027);\n zoomModalEl.setAttribute(\u0027aria-hidden\u0027, \u0027true\u0027);\n let html = `\n \u003Cdiv class=\u0022modal-dialog\u0022\u003E\n \u003Cdiv class=\u0022modal-content\u0022\u003E\n \u003Cdiv class=\u0022modal-body\u0022\u003E\n \u003Cdiv id=\u0022${id}\u0022 class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022 data-interval=\u0022\u0022\u003E\n \u003C!-- Wrapper for slides --\u003E\n \u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E${items}\u003C\/div\u003E\n\n \u003C!-- Controls --\u003E\n \u003Ca class=\u0022left carousel-control\u0022 href=\u0022#${id}\u0022 role=\u0022button\u0022 data-slide=\u0022prev\u0022\u003E\n \u003Cspan class=\u0022glyphicon glyphicon-chevron-left\u0022 aria-hidden=\u0022true\u0022\u003E\u003C\/span\u003E\n \u003Cspan class=\u0022sr-only\u0022\u003EPrevious\u003C\/span\u003E\n \u003C\/a\u003E\n \u003Ca class=\u0022right carousel-control\u0022 href=\u0022#${id}\u0022 role=\u0022button\u0022 data-slide=\u0022next\u0022\u003E\n \u003Cspan class=\u0022glyphicon glyphicon-chevron-right\u0022 aria-hidden=\u0022true\u0022\u003E\u003C\/span\u003E\n \u003Cspan class=\u0022sr-only\u0022\u003ENext\u003C\/span\u003E\n \u003C\/a\u003E\n \u003C\/div\u003E\n \u003Cdiv class=\u0022abm-mt-3\u0022\u003E\n \u003Cbutton type=\u0022button\u0022 class=\u0022btn abm-btn abm-w-100\u0022 data-dismiss=\u0022modal\u0022\u003EClose\u003C\/button\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/div\u003E\n `;\n zoomModalEl.innerHTML = html;\n document.body.appendChild(zoomModalEl);\n const $el = $(zoomModalEl);\n $el.modal(\u0027show\u0027);\n $el.on(\u0027hidden.bs.modal\u0027, function () {\n zoomModalEl.remove();\n });\n }\n \/\/----------------\n \/\/ \u56fe\u7247\u653e\u5927 END\n \/\/----------------\n\n window.onpopstate = function () {\n \/\/ \u76f4\u63a5\u8fd4\u56de\u4e0a\u4e00\u7ea7\u9875\u9762\n history.back();\n return false;\n if (!history.state) {\n history.back();\n }\n search();\n }\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"Immortalized Cells","meta_keywords":"Immortalized cell lines, Cell biology, Cell culture, abm, Primary cells, Mycoplasma detection and elimination, Growth factors and cytokines, Cell immortalization service, Cell characterization, Cell culture methods","meta_description":"abm offers over 400 immortalized cell lines from various organisms, tissue systems, and tissues1. Discover our high-quality and affordable solutions for your cell biology research.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":"abm_catalog_cellular","image":null,"independentPage":0,"top_type":1,"sort_order":201,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-04-05 01:14:02"},"info":{"id":849,"cat_no_base":"T0788","parent_id":40630,"image":null,"price-original":null,"description":"\u003Cp\u003ENCL-2 is the only mast line cell line existing without Kit gene mutation derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth signals. Moreover, NCL-2 cells expressed \u003Cstrong\u003EFceRI and KIT\u003C\/strong\u003E, and release histamine and LTB4 in response to antigen stimulation. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.\u003C\/p\u003E","shipment_type":null,"internal_leadtime":"0","lysate_type":null,"small_image":null,"growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003EMost immortalized mast cell lines are tumour cell lines, and carry a gain-of-function mutation of Kit. Immortalized Mast Cells (NCL-2) is derived from NC mouse bone marrow, and does not carry a Kit mutation. NLC-2 can respond to exogenous growth signals SCF and IL-3, and releases various chemical mediators against several stimuli including crosslinking of Fc \u03b5RI. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"MEM-alpha + 10% FBS + 50 uM B-me + 2 mM L-glu + 5 ng\/mL IL-3\r\n33 degree","alias":null,"tier_price":null,"operator_no_use":null,"addtime":null,"cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":"Round","expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":null,"cell_morphology_specification":null,"tax_class_id":2,"inventory_location":"D10R1B1 (B1-B5); D5 (E3, E4, F5-F6)","vector":null,"internal_note":null,"component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":null,"seeding_density_ml":"150,000 - 200,000","source":null,"source_catno":"3950\/7950","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":"30","source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":null,"donor_gender":"Male","appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":"2","biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":"Spontaneous immortalization","bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003ESuspension, round\u003C\/p\u003E","expression_profile":"\u003Cp\u003EFc \u0026epsilon;RI, KIT\u003C\/p\u003E","storage":null,"propagation_method":"\u003Cp\u003EThe base medium for this cell line is Prigrow VIII medium available at\u0026nbsp;\u003Cstrong\u003Eabm\u003C\/strong\u003E, Cat. No.\u003Ca href=\u0022\/prigrow-viii-medium-tm018.html#TM018\u0022\u003ETM018\u003C\/a\u003E\u0026nbsp;or Prigrow II medium available at\u0026nbsp;\u003Cstrong\u003Eabm\u003C\/strong\u003E, Cat. No.\u003Ca href=\u0022\/prigrow-ii-medium-tm002.html#TM002\u0022\u003ETM002\u003C\/a\u003E. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (\u003Ca href=\u0022\/usda-research-grade-origin-fetal-bovine-serum-tm999-500.html#TM999\u0022\u003ETM999\u003C\/a\u003E)* to a final concentration of 10%, Penicillin\/Streptomycin Solution (\u003Ca href=\u0022\/penicillin-streptomycin-solution-g255.html#G255\u0022\u003EG255\u003C\/a\u003E) to a final concentration of 1%, \u0026beta;-mercaptoethanol to a final concentration of 50 \u0026mu;M, L-glutamine (\u003Ca href=\u0022\/l-glutamine-g275.html#G275\u0022\u003EG275\u003C\/a\u003E) to a final concentration of 2 mM, and Recombinant Mouse IL3 (\u003Ca href=\u0022\/recombinant-mouse-il3-e-coli-10000273.html#Z200145\u0022\u003EZ200145\u003C\/a\u003E) to a final concentration of 5 ng\/mL.\u003Cbr \/\u003ECarbon dioxide (CO\u003Csub\u003E2\u003C\/sub\u003E): 5%, Temperature: 33.0\u0026deg;C.\u003Cbr \/\u003E\u003Cstrong\u003E* Do not use heat-inactivated FBS for cell culture unless specified otherwise.\u003C\/strong\u003E\u003C\/p\u003E","usage":null,"freeze_thaw_recovery":null,"str_profile":null,"str_markers":"","subculture_protocol":"\u003Cp\u003E1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10\u2076 cells\/ml.\u003C\/p\u003E\n\u003Cp\u003E2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.\u003C\/p\u003E\n\u003Cp\u003E3. Incubate the cells at the recommended conditions.\u003C\/p\u003E","preservation_protocol":"1. Freeze Medium: 90% FBS and 10% DMSO.\u003Cbr\u003E2. Storage Temperature: Liquid nitrogen vapour phase.","passage_number":null,"qc":"\u003Cp\u003E1) Flow cytometry\u003C\/p\u003E","shipping_conditions":"\u003Cp\u003EShip with dry ice.\u003C\/p\u003E","disclaimer_general":"\u003Cp\u003E1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each cell line. If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location.\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic or any other non-RUO application(s).\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period\u0022.\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":"Hiroshima University","licensing_institution":null,"guarantee":null,"depositor":"Hiroshima University","licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":"ABM","internal_product_note":"\u003Cp\u003ELicensed from: Hiroshima University\u003C\/p\u003E","donor_history":"Male, NC mouse","split_ratio":null,"expression":"\u003Cp\u003EFc \u0026epsilon;RI, KIT\u003C\/p\u003E","growth_conditions":"PriGrow VIII (\u003Ca class=\u0022abm-link\u0022 href=\u0022\/prigrow-viii-medium-tm018.html\u0022\u003ETM018\u003C\/a\u003E) + 10% FBS + 50 \u03bcM \u03b2-mercaptoethanol + 2 mM L-glutamine + 5 ng\/ml IL3 (Z200145) + 1% Penicillin\/Streptomycin Solution (\u003Ca class=\u0022abm-link\u0022 href=\u0022\/penicillin-streptomycin-solution-g255.html\u0022\u003EG255\u003C\/a\u003E), 33.0\u00b0C, 5% CO\u2082","recommend":"\u003Cp\u003EWorried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":"\u003Cp\u003EHiragun, T., Yanase, Y., Okabe, T., Hiragun, M., Kawai, M., \u0026amp; Hide, M. (2014). Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow. FEBS open bio, 4, 342-6. doi:10.1016\/j.fob.2014.03.011\u003C\/p\u003E","cryopreservation":"\u003Cp\u003ECryopreservation Medium (TM024), or complete growth media with 10% DMSO.\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","promotions":null,"sync_images":"","thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-07 08:13:18","updated_at":"2025-02-13 19:02:35","concentration":null},"seo":{"id":66681,"catalog_id":40630,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"immortalized-mast-cells-ncl-2.html","meta_title":"Immortalized Mast Cells (NCL-2)","meta_keywords":"Immortalized Mast Cells (NCL-2)","meta_description":"Immortalized Mast Cells (NCL-2)","created_at":"2022-09-23 06:53:13","updated_at":"2025-02-13 19:02:35"},"media":[{"id":388467,"parent_id":40630,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/rHyKxU1nKR6jFHFWh1QxKpDWv1uL5QmtmtHqiEv1.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-18 19:00:05","updated_at":"2025-02-18 19:00:05"},{"id":388291,"parent_id":40630,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/dPmAy3yBx4LvigMwRT2uvIsh2naryQ97Zd8VZEJp.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-18 18:58:44","updated_at":"2025-02-18 18:58:50"},{"id":388213,"parent_id":40630,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/MQRLklawcmDLeVmIFxRPdH3VYmxhzQWJd8ygKQrg.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-13 19:02:32","updated_at":"2025-02-18 18:58:50"}],"gene":null}