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General Materials

MegaFi™ Pro Fidelity 2X PCR MasterMix

Cat. No.

G887

Unit

800 rxn (10.0 ml)

Price

$335.00

Cat. No.

G887

Name

MegaFi™ Pro Fidelity 2X PCR MasterMix

Unit

800 rxn (10.0 ml)

Category

PCR Polymerase

Description

MegaFi™ Pro Fidelity 2X PCR MasterMix sets a new benchmark for specific, sensitive, robust, and high-fidelity PCR performance in a uniquely-formaulated buffer with gel loading dye. This advanced, strategically engineered enzyme is combined with a monoclonal antibody that inhibits both 5’-3’ polymerase activity and 3'-5’ exonuclease activity at room temperature, enabling precise Hot Start reactions. With exceptional sensitivity, MegaFi™ Pro can amplify even the most challenging templates, outperforming leading competitors. It delivers unparalleled high fidelity with an ultra-low error rate—over 2,000 times lower than Taq polymerase—making it the most accurate enzyme available. Ideal for demanding applications such as Next Generation Sequencing, molecular cloning, and diagnostics, MegaFi™ Pro ensures the highest quality results for your PCR needs.

Product Features:

2000X lower error rates than regular Taq
Amplifies difficult templates
Blunt ends
Contains gel loading dye
Included in abm's PCR Buffet Program

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Application

Ultra high-fidelity PCR
Next Generation Sequencing
Accurate CRISPR edit detection
Mutagenesis

Concentration

Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G887

Datasheet

MegaFi™ Pro Fidelity 2X PCR MasterMix Datasheet

Search CoA here

MSDS

MegaFi™ Pro Fidelity 2X Mastermix MSDS

Other

	What are the advantages of using a High-Fidelity DNA Polymerase?
	MegaFi Pro has high fidelity and high processivity. This means it has low error rates leading to more accurate sequences and longer amplicons leading to a faster reaction time. High fidelity is achieved due to its strong proofreading exonuclease activity, making it the preferred polymerase for all PCR applications requiring greater accuracy or the amplification of difficult templates.

	What ends will my PCR products have?
	Blunt ends, this makes it not suitable for TA cloning

	What does 3' - 5' exonuclease activity mean?
	It is the proofreading activity which digests nucleotides with 3´ hydroxyl groups from the 3´ to 5´ direction. This includes correction of mismatched base pairs and terminal 3´ digestion.

	Does the dye in the MasterMix affect downstream applications?
	The loading dye does not interfere with downstream cloning experiments. However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.

How do you determine the fidelity of MegaFi™ Pro?

The fidelity of MegaFi Pro has been tested through Blue-white screening assay.

LacZa gene was amplified using MegaFi™ Pro, NEB's Q5, Vazyme's PhantaMax, and Native Taq. The PCR amplicons were then subcloned into pUC19 vector and plated into a X-gal containing agar plate. Any mutations from PCR will cause the colonies to turn while no mutation will remain blue. Blue and white colonies were counted and the error rate per base was calculated and compared to native Taq.

I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue?

The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.

	Can I store my PCR reaction mix once it has been assembled?
	It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.

	What discontinued abm product is Cat. No. G887 equivalent to?
	This product is abm’s next generation of DNA Polymerases and is functionally equivalent to Cat. G124, G124-dye, G464, G464-dye, G497, G497-dye, G886, and G897 (Precision, Bestaq, Kodaq, and MegaFi PCR MasterMixes), with improved performance. Contact our customer service team technical@abmgood.com for more information.

Bi, L., Zhang, X., Zou, S., Ji, D., & Zhang, Q. (2022). Detection of prokaryotes on the astomatous ciliated protist Kentrophoros flavus (Ciliophora, Karyorelictea) revealed a consistently associated muribaculaceae-like bacterium. Frontiers in Marine Science, 9, 879388. https://doi.org/10.3389/fmars.2022.879388

Deng, J., Deng, X., Yao, H., Ji, S., & Dong, L. (2024). Gibberellins Play an Essential Role in the Bud Growth of Petunia hybrida. Current Issues in Molecular Biology, 46(9), 9906-9915. https://doi.org/10.3390/cimb46090590

Deng, J., Deng, X., Zhang, M., Tang, Z., & Dong, L. (2025). PhSLB1 Regulates Branch Development in Petunia. Journal of the American Society for Horticultural Science, 150(1), 19-27. https://doi.org/10.21273/JASHS05413-24

Dong, L., Xu, X., Jiang, Z., Yao, H., Tian, W., & Deng, X. (2023). PhCYP724B1 positively regulates shoot branching. https://doi.org/10.21203/rs.3.rs-3165454/v1

Du J, Yin H, Li J, Zhang W, Ding G, Zhou D, Sun Y, Shen B. Transcription factor B-H2 regulates CYP9J34 expression conveying deltamethrin resistance in Culex pipiens pallens. Pest Manag Sci. 2024 Apr;80(4):1991-2000. https://doi.org/10.1002/ps.7934 E pub 2024 Jan 18. PMID: 38092527.

Ferreira, P., Kwan, G. T., Haldorson, S., Rummer, J. L., Tashiro, F., Castro, L. F. C., ... & Wilson, J. M. (2022). A multi-tasking stomach: functional coexistence of acid–peptic digestion and defensive body inflation in three distantly related vertebrate lineages. Biology letters, 18(2), 20210583. https://doi.org/10.1098/rsbl.2021.0583

Jiang, Z., Liu, T., Zhang, Q., Dong, L., & Deng, X. (2023). PhNAL1 Is Involved in Regulating Branch Development of Petunia. Journal of the American Society for Horticultural Science, 148(6), 276-282. https://doi.org/10.3390/horticulturae10090912

Kim, H. K., Kim, C. J., Jang, D., & Lim, D. H. (2023). MicroRNA miR-274-5p suppresses found-in-neurons associated with melanotic mass formation and developmental growth in Drosophila. Insects, 14(8), 709. https://doi.org/10.3390/insects14080709

Kolátková, V., Mooney, M., Kelly, K., Hineva, E., Gawryluk, R. M., & Elliott, J. (2023). Eelgrass (Zostera spp.) associated phytomyxids are host‐specific congeneric parasites and predominant eukaryotes in the eelgrass rhizosphere on a global scale. Environmental Microbiology, 25(8), 1522-1537. https://doi.org/10.1111/1462-2920.16376

Lee, S., Le, Q. T., Yang, S., Hwang, K. Y., & Lee, H. (2023). Arabidopsis ecotype Ct-1, with its altered nitrate sensing ability, exhibits enhanced growth under low nitrate conditions in comparison to Col-0. Plant Science, 336, 111827. https://doi.org/10.1016/j.plantsci.2023.111827

Liu, C., Yang, P., Wang, X., Xiang, B., E, G., & Huang, Y. (2024). Candidate circRNAs related to skeletal muscle development in Dazu black goats. Animal Biotechnology, 35(1), 2286609. https://doi.org/10.1080/10495398.2023.2286609

Mera Barrera, M. B., & Pardo Calva, D. F. (2025). Detección del gen blaCTX-M grupo 1 en enterobacterales productores de betalactamasas de espectro extendido en cepas procedentes de la Clínica Santa Ana y Hospital del Río, Cuenca 2024. https://dspace.ucuenca.edu.ec/handle/123456789/46278

Peng, K., Sun, A., Zhu, J., Gao, J., Li, Y., Shao, G., ... & Lin, Q. (2021). Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs. Oncology Letters, 22(3), 638. https://doi.org/10.3892/ol.2021.12899

Wu, W., Li, C., Zhang, Q., Wang, Y., Li, X., Yang, Q., & Dong, L. (2024). PhSPEAR1 Participates in Regulating the Branch Development of Petunia. Horticulturae, 10(9), 912. https://doi.org/10.3390/horticulturae10090912

Wu, X., Alibayov, B., Xiang, X., Lattar, S. M., Sakai, F., Medders, A. A., ... & Vidal, J. E. (2024). Ultrastructural, metabolic and genetic characteristics of determinants facilitating the acquisition of macrolide resistance by Streptococcus pneumoniae. Drug Resistance Updates, 77, 101138. https://doi.org/10.1016/j.drup.2024.101138

Yang, Y., Cai, Q., Luo, L., Sun, Z., & Li, L. (2024). Genome-Wide Analysis of C-Repeat Binding Factor Gene Family in Capsicum baccatum and Functional Exploration in Low-Temperature Response. Plants, 13(4), 549. https://doi.org/10.3390/plants13040549

Yang, Y., Cai, Q., Wang, Y., Li, L., & Sun, Z. (2024). Genome-wide exploration of the WD40 gene family in eggplant (Solanum melongena L.) and analysis of its function in fruit color formation. Agronomy, 14(3), 521. https://doi.org/10.3390/agronomy14030521

Yi, S., Cai, Q., Yang, Y., Shen, H., Sun, Z., & Li, L. (2024). Identification and Functional Characterization of the SaMYB113 Gene in Solanum aculeatissimum. Plants, 13(11), 1570. https://doi.org/10.3390/plants13111570

Zhang, F., Song, W., Yang, R., Jin, C., Xie, Y., Shen, Y., ... & He, Y. (2024). Semen promotes oocyte development in Sebastes schlegelii elucidating ovarian development dynamics in live-bearing fish. Iscience, 27(3). https://doi.org/10.1016/j.isci.2024.109193

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No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/PCR-Sure-Kit.html\u0022\u003EPCR Sure\u0026trade; Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/PCR-Sure-Kit.html\u0022\u003EG065\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E8 x 10 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G065\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Versatile\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EAmplify your difficult templates with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s PCR Sure\u0026trade; Kit. From crude templates to purified plasmids, High GC to Low GC, human, mouse, rat or plant DNA, one of our highly optimized mastermix is sure to work!\u003C\/p\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-sure-featured-product-page-figure-1.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-sure-featured-product-page-figure-2.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExtremely Easy to Use\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EWith 8 optimized 2X MasterMixes, simply add your primers and template and run on one single program!\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/PCR-Sure-tube-image.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022MegaFi\u0026trade; Pro\u0022 id=\u0022MegaFi\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EMegaFi\u0026trade; Pro Fidelity DNA Polymerase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EMegaFi\u0026trade; Pro Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 2000X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/high-fidelity-icon.png\u0022 \/\u003E\n\u003Cp\u003EMegaFi\u0026trade; Pro Fidelity\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/amplifies-difficult-templates-icon.png\u0022 \/\u003E\n\u003Cp\u003EAmplifies difficult templates\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/effective-in-long-range-pcr-icon.png\u0022 \/\u003E\n\u003Cp\u003EEffective in long-range PCR\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-megafi-fidelity-product-photo-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-pro-fidelity-dna-polymerase.html\u0022\u003EMegaFi\u0026trade; Pro Fidelity DNA Polymerase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-pro-fidelity-dna-polymerase.html\u0022\u003EG886\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E400 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G886\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-pro-fidelity-2x-pcr-mastermix.html\u0022\u003EMegaFi\u0026trade; Fidelity 2X PCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-pro-fidelity-2x-pcr-mastermix.html\u0022\u003EG887\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E800 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G887\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafitm-one-step-rt-pcr.html#g597\u0022\u003EMegaFi\u0026trade; Pro One-Step RT-PCR *\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafitm-one-step-rt-pcr.html#g597\u0022\u003EG597\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G597\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd colspan=\u00224\u0022\u003E*Contains \u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EOneScript\u0026trade; Hot Reverse Transcriptase (Cat. No. G593)\u003C\/a\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EUltra-Low Error Rates\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/lmopgadochmjhaaf.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EMegaFi\u0026trade; Pro DNA Polymerase is THE BEST high fidelity DNA polymerase\u003C\/strong\u003E A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuperior Fidelity\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/ifnegnebipmbfiee.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EMegaFi\u0026trade; Pro Fidelity DNA Polymerase has ~2000X higher fidelity than other enzymes on the market. \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s\u003C\/strong\u003E MegaFi\u0026trade; Pro Fidelity DNA Polymerase (Cat. No. G886) has the highest accuracy rate compared to other DNA polymerases as tested using blue\/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022Kodaq 2X MasterMix\u0022 id=\u0022Kodaq\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EKodaq 2X PCR MasterMix\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EKodaq 2X PCR MasterMix has a ready-to-use formula which includes a uniquely-formulated buffer with gel loading dye and strategically engineered mutations for robust, high-fidelity performance. The buffer accommodates high AT and GC content and common PCR inhibitors found in DNA samples.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/kodaq-long-range.png\u0022 \/\u003E\n\u003Cp\u003ELong Range PCR\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/kodaq-ready-to-use.png\u0022 \/\u003E\n\u003Cp\u003EHighly Robust\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/kodaq-robust.png\u0022 \/\u003E\n\u003Cp\u003ESuperior sensitivity\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-kodaq-product-photo-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/Kodaq-PCR-MasterMix.html\u0022\u003EKodaq 2X PCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/Kodaq-PCR-MasterMix.html\u0022\u003EG883\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E800 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G883\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/Kodaq-figure-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Ch2 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cb\u003EHighly Robust\u003C\/b\u003E\u003C\/h2\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EPCR amplification of a GC-Rich 1.4 kbp plasmid template using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s Kodaq 2X PCR MasterMix (Cat. No. G883) (column 1) vs. competitor products (column 2 to 5).\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/Kodaq-figure-2.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EPCR amplification of difficult templates (including plant gDNA) using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s Kodaq 2X PCR MasterMix (Cat. No. G883) (column 1) vs. competitor products (column 2 and 3). The red arrows indicate the expected product sizes.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022BlasTaq\u0026trade;\u0022 id=\u0022BlasTaq\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; DNA Polymerase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EBlast through a routine 2 hour PCR run in only 20 minutes with our BlasTaq\u0026trade; DNA Polymerase. Reach extension speeds as fast as 6 kb\/min and streamline your PCR!\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/extension-speed-icon.png\u0022 \/\u003E\n\u003Cp\u003E6 kb\/min extension speed\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/reaction-time-icon.png\u0022 \/\u003E\n\u003Cp\u003E40-70% less time than Taq\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/superior-sensitivity-icon.png\u0022 \/\u003E\n\u003Cp\u003ESuperior sensitivity\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-blastaq-product-photo-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-dna-polymerase.html#g894\u0022\u003EBlasTaq\u0026trade; DNA Polymerase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-dna-polymerase.html#g894\u0022\u003EG894\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E400 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G894\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-pcr-taq-mastermix.html\u0022\u003EBlasTaq\u0026trade; 2X PCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-pcr-taq-mastermix.html\u0022\u003EG895\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E800 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G895\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Fast Extension Speeds\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-blastaq-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; DNA Polymerase enables extension speeds as fast as 6 kb\/min.\u003C\/strong\u003E Total reaction times for \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s BlasTaq\u0026trade; DNA Polymerase (Cat. No. G894) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EHighly Robust\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-blastaq-data-2.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EPCR amplification using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s BlasTaq\u0026trade; DNA Polymerase (Cat. No. G894) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022BlasTaq\u0026trade; HotStart\u0022 id=\u0022BlasTaqHotStart\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; HotStart DNA Polymerase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EEliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq\u0026trade; HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/non-specific-amplification-icon.png\u0022 \/\u003E\n\u003Cp\u003ENo non-specific amplification or primer dimers\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/high-yield-icon.png\u0022 \/\u003E\n\u003Cp\u003EImproves yield of desired product\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/simplifies-reaction-setup-icon.png\u0022 \/\u003E\n\u003Cp\u003ESimplifies reaction setup\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-hotstart-product-photo-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-hotstart-dna-polymerase.html\u0022\u003EBlasTaq\u0026trade; HotStart DNA Polymerase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-hotstart-dna-polymerase.html\u0022\u003EG595\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E400 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G595\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-hotstart-2X-mastermix.html\u0022\u003EBlasTaq\u0026trade; HotStart 2X PCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-hotstart-2X-mastermix.html\u0022\u003EG598\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E800 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G598\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent for suspension cells\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-hotstart-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; HotStart DNA Polymerase eliminates non-specific amplification. abm\u003C\/strong\u003E \u0026rsquo;s BlasTaq\u0026trade; HotStart DNA Polymerase (Cat. No. G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is our BlasTaq\u0026trade; HotStart formulation and lane 5 contains no antibody.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Fast Extension Speeds\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-hotstart-data-2.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; HotStart DNA Polymerase has extension speeds of 6 kb\/min. abm\u003C\/strong\u003E \u0026rsquo;s BlasTaq\u0026trade; HotStart DNA Polymerase (Cat. No. G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022Ultra-Pure Blastaq\u0026trade;\u0022 id=\u0022UltraPure\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUltra-Pure BlasTaq\u0026trade; 2X PCR MasterMix\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ETake your PCR to the next level with \u003Cstrong\u003EUltra-Pure BlasTaq\u0026trade; 2X PCR MasterMix\u003C\/strong\u003E. This formulation includes the same strategically-engineered, next generation Taq Polymerase which was subject to rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/ultra-pure-high-purity.png\u0022 \/\u003E\n\u003Cp\u003EHigh purity\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/ultra-pure-speed.png\u0022 \/\u003E\n\u003Cp\u003E6 kb\/min extension speed\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/ultra-pure-fast.png\u0022 \/\u003E\n\u003Cp\u003E40-70% less time than Taq\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/pcr-polymerase-ultra-pure-product-photo-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/catalog\/products\/3363\/type\/molecular\u0022\u003EUltra-Pure BlasTaq\u0026trade; 2X PCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/catalog\/products\/3363\/type\/molecular\u0022\u003EG885\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G885\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EHigh Purity\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/ultra-pure-blastaq-image.png\u0022 \/\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EUltra-Pure BlasTaq\u0026trade; PCR MasterMix ensures efficient amplification while maintaining low-DNA contamination levels when compared to leading competitors.\u003C\/strong\u003E Two different primer sets (blue and orange) against host DNA were tested with two competitor products (1 and 2) and \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s Ultra-Pure BlasTaq\u0026trade; enzyme (3). There is no amplification seen in the no-template controls (NTC) from Ultra-Pure BlasTaq\u0026trade; PCR MasterMix indicating exceptional purity of the product. Activity Control Primer Set demonstrated similar performance for all three tested products.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div abm-page-left-link\u0022 data-text=\u0022Unsure which polymerase is right for you?\u0022 id=\u0022Unsure-which-polymerase-is-right-for-you\u0022\u003E\n\u003Ch2 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cb\u003EUnsure which polymerase is right for you?\u003C\/b\u003E\u003C\/h2\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which polymerase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple PCR Enzyme Selection Guide below to compare the characteristics of each polymerase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022width: 100%; height: 176px; --child2-white-space: normal; --last-child-white-space: normal; --first-child-width: auto;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Cth style=\u0022width: 14.29%; height: 25px; text-align: center; vertical-align: middle;\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth style=\u0022width: 14.29%; height: 25px; text-align: center; vertical-align: middle;\u0022\u003E\u003Ca href=\u0022#MegaFi\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EMegaFi\u0026trade; Pro Fidelity\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth style=\u0022width: 14.29%; height: 25px; text-align: center; vertical-align: middle;\u0022\u003E\u003Ca href=\u0022#BlasTaq\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EBlasTaq\u0026trade;\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth style=\u0022width: 14.29%; height: 25px; text-align: center; vertical-align: middle;\u0022\u003E\u003Ca href=\u0022#BlasTaqHotStart\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EBlasTaq\u0026trade; 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vertical-align: middle;\u0022\u003E15 sec\/kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E60 sec\/kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E60 sec\/kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003EMax Target Length\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E15-20 kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E10-15 kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E10-15 kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E10-15 kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E6.5 kb\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003E15-20 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003ESuitable for TA cloning\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003ENo\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003EYes\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003EYes\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003EYes\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003EYes\u003C\/td\u003E\n\u003Ctd style=\u0022height: 25px; text-align: center; vertical-align: middle;\u0022\u003ENo\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022height: 25px; 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Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1600px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-introduction\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/Resources_DNA-Polymerase-Variations.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to PCR\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"PCR Polymerase","meta_keywords":null,"meta_description":null,"deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":102,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":963,"cat_no_base":"G887","parent_id":118,"description":"\u003Cp\u003E\u003Cstrong\u003EMegaFi\u0026trade; Pro Fidelity 2X PCR MasterMix\u003C\/strong\u003E sets a new benchmark for \u003Cstrong\u003Especific\u003C\/strong\u003E, \u003Cstrong\u003Esensitive\u003C\/strong\u003E,\u003Cstrong\u003E robust\u003C\/strong\u003E, and \u003Cstrong\u003Ehigh-fidelity\u003C\/strong\u003E PCR performance in a uniquely-formaulated buffer with gel loading dye. This advanced, strategically engineered enzyme is combined with a \u003Cstrong\u003Emonoclonal antibody\u003C\/strong\u003E that inhibits both 5\u0026rsquo;-3\u0026rsquo; polymerase activity and 3\u0027-5\u0026rsquo; exonuclease activity at room temperature, enabling precise Hot Start reactions. With exceptional \u003Cstrong\u003Esensitivity\u003C\/strong\u003E, MegaFi\u0026trade; Pro can amplify even the most challenging templates, outperforming leading competitors. It delivers unparalleled \u003Cstrong\u003Ehigh fidelity\u003C\/strong\u003E with an \u003Cstrong\u003Eultra-low error rate\u003C\/strong\u003E\u0026mdash;over \u003Cstrong\u003E2,000 times lower \u003C\/strong\u003Ethan Taq polymerase\u0026mdash;making it the most accurate enzyme available. 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