Mycoplasma Pro PCR Detection Kit~10 min
- Prepare master mix
- Add 2.5 µL culture supernatant
- Prepare PCR samples
Competitor Comparison
Mycoplasma Pro PCR Detection Kit vs Competing PCR Kits
Cat. No. G239See how abm's Mycoplasma Pro PCR Detection Kit compares with two competing PCR assays across sample preparation, time to results, reaction setup, and gel interpretation.
~10 minsample preparation
1 hr 20 mintotal time to results
1 reactionper sample
100reactions per kit
Key Differences at a Glance
The main differences are workflow time, reaction setup, and how much interpretation is required at the gel.
Less preparation~10 min · 3 pre-PCR steps
Faster result1 hr 20 min total
1 reaction per sampleCompetitor A requires 2
| What matters | Competitor A | Competitor S | Mycoplasma Pro PCR Detection Kit |
|---|---|---|---|
| Sample preparation | ~60 min · 7 steps | ~20 min · 5 steps | ~10 min · 3 steps |
| Total time to results | 2 hr 37 min | 2 hr 31 min | 1 hr 20 min |
| Reactions per sample | 2 reactions | 1 reaction | 1 reaction |
| How results are read | Compare paired sample + confirmation lanes | Read 481 bp internal control + 259 bp positive band | Read 180 bp internal control + 300–600 bp positive range in one lane |
A Shorter Path to Results
The Mycoplasma Pro PCR Detection Kit combines shorter sample preparation with a shorter PCR run.
1 hr 20 min77 min faster than Competitor A · 71 min faster than Competitor S
Sample preparationPCR run
Mycoplasma Pro PCR Detection KitDirect-supernatant workflow
1 hr 20 minfastest reported total
Competitor ACell-harvest workflow
2 hr 37 min77 min longer
Competitor SHeat-and-spin workflow
2 hr 31 min71 min longer
What happens before PCR?
Competitor A~60 min
- Scrape cells
- Spin (3 min)
- Add lysis buffer
- Incubate (15 min)
- Heat (10 min)
- Spin (5 min)
- Prepare PCR
Competitor S~20 min
- Heat (5 min)
- Centrifuge
- Prepare master mix
- Prepare samples
- Incubate (5 min)
Same Results. Different Readout.
All three assays reached the same sample calls. The difference is what has to be read before you can make that call.
Same calls: 1 Negative 2 Positive 3 Positive 4 Positive
Competitor A2 reactions / sample
What you read
Lane 1Sample
+
Lane 2Sample + positive control
Compare both lanes
2 lanesEach sample requires a paired-lane comparison.
Competitor S1 reaction / sample
What you read in one lane
481 bp Internal control 259 bp Positive-result band
Strong target ↑Internal control may weaken ↓
2 signalsSignal balance can vary in strongly positive samples.
Mycoplasma Pro PCR Detection Kit1 reaction / sample
What you read in one lane
300–600 bp Positive-result region 180 bp Internal control
Separated regions in one lane
1 laneClearly separated result regions make the readout straightforward.
See the detailed gel readouts belowThe annotated gels show the exact bands, controls, and lane setup behind each readout.
Technical Comparison
| Comparison point | Mycoplasma Pro PCR Detection Kit | Competitor A | Competitor S |
|---|---|---|---|
| Sample type and amount | Cell supernatant, 2.5 µL | Cell suspension, 1 mL | Cell supernatant, 100 µL |
| Reactions per sample | 1 | 2 | 1 |
| Reactions per kit | 100 | 40 | 24 |
| Sample preparation time | ~10 min | ~1 hour | ~20 min |
| PCR run time | 1 hour 10 min | 1 hour 37 min | 2 hours 11 min |
| Total time to results | 1 hour 20 min | 2 hours 37 min | 2 hours 31 min |
| Internal PCR control | Yes | No | Yes |
| Target coverage | 300+ | 60+ | Not specified |
| Reported sensitivity | 10 CFU/mL | 2 fg at 32 cycles | ≤20 mycoplasma genomes per 2 mL sample |
| Result readout | 180 bp internal control + 300–600 bp positive-result range in the same sample reaction | Positive result at 434–468 bp; separate S,+ reaction is used for confirmation | 481 bp internal control + 259 bp positive result; internal-control signal can weaken in strongly positive samples |
| Additional materials required | None reported | Heat block and centrifuge | Taq DNA polymerase, heat block, and centrifuge |
Reported sensitivity values use different units and test conditions and are not a normalized head-to-head analytical comparison.
Explore the Mycoplasma Detection Options from abm
Compare the Mycoplasma Pro PCR Detection Kit with the full range of PCR and qPCR mycoplasma detection kits.