pLenti-5-UTR-GFP

                            LOCUS       dna                     10250 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2439..2444
                     /dbxref="REBASE:ClaI"
     misc_binding    3425..3430
                     /dbxref="REBASE:EcoRI"
     ORF             3850..4563
                     /sequence="ORF_6 rf(6)"
     ORF             4430..5359
                     /sequence="ORF_5 rf(5)"
     misc_binding    4469..4474
                     /dbxref="REBASE:EcoRV"
     misc_binding    4490..4495
                     /dbxref="REBASE:BamHI"
     misc_binding    4496..4501
                     /dbxref="REBASE:XbaI"
     Reporter        4520..5236
                     /gene="EGFP reporter"
     ORF             4520..5260
                     /sequence="ORF_3 rf(2)"
     Promoter        5311..5579
                     /gene="SV40 prom"
     Rep_Origin      5478..5555
                     /gene="SV40 origin"
     ORF             5545..6213
                     /sequence="ORF_2 rf(1)"
     misc_binding    5558..5570
                     /dbxref="REBASE:SfiI"
     Marker          5614..6213
                     /gene="puro marker"
     Other Gene      6823..6875
                     /gene="delta_U3 other"
     Other Gene      6876..7056
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7562..8181
                     /gene="pBR322 origin"
     Promoter        8834..8883
                     /gene="NEOKAN prom"
     misc_binding    8941..8946
                     /dbxref="REBASE:BclI"
     ORF             8972..9766
                     /sequence="ORF_4 rf(2)"
     Marker          8975..9763
                     /gene="NTP_II marker"
     Rep_Origin      9871..10176
                     /gene="f1 origin"
BASE COUNT    2408 a   2828 c   2885 g   2129 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg ggggagctca     
     2461 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg     
     2521 gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat cccgagccgg     
     2581 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg     
     2641 cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag gtaccgggga     
     2701 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct     
     2761 agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc cgcatcccga     
     2821 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct     
     2881 gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg aaaacaattc     
     2941 ggggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc     
     3001 ccgctagggg gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat     
     3061 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt     
     3121 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag     
     3181 gtaccgggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc     
     3241 ctcccccgct agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc     
     3301 cgcatcccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc     
     3361 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg     
     3421 aaaagaattc atagcagaca tacaacggac ggtgggccca gacccaggct gtgtagaccc     
     3481 agcccccccg ccccgcagtg cctaggtcac ccactaacgc cccaggcctt gtcttggctg     
     3541 ggcgtgactg ttaccctcaa aagcaggcag ctccagggta aaaggtgccc tgccctgtag     
     3601 agcccacctt ccttcccagg gctgcggctg ggtaggtttg tagccttcat cacgggccac     
     3661 ctccagccac tggaccgctg gcccctgccc tgtcctgggg agtgtggtcc tgcgacttct     
     3721 aagtggccgc aagccacctg actcccccaa caccacactc tacctctcaa gcccaggtct     
     3781 ctccctagtg acccacccag cacatttagc tagctgagcc ccacagccag aggtcctcag     
     3841 gccctgcttt cagggcagtt gctctgaagt cggcaagggg gagtgactgc ctggccactc     
     3901 catgccctcc aagagctcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg     
     3961 cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg     
     4021 tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg     
     4081 ggccgaaccg ggcactgccc agcgtggggc gcgggggcca cggcgcgcgc ccccagcccc     
     4141 cgggcccagc accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct     
     4201 cgctctcgct cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac     
     4261 tgtgcggcga agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag     
     4321 ttgcctttta tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg     
     4381 ccaccaccgc cgagaccgcg tccgccccga gatctcagag cctcgccttt catatcggcg     
     4441 cgccactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag     
     4501 actgcagctc gaggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc     
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg     
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct     
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg     
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt     
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa     
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga     
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat     
     4981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga     
     5041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt     
     5101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga     
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat     
     5221 ggacgagctg tacaaggggc accaccacca ccaccactga gtcgagtacc catacgacgt     
     5281 cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag     
     5341 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag     
     5401 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc     
     5461 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc     
     5521 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat     
     5581 tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc cacggtgcgc     
     5641 ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac     
     5701 taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt caccgagctg     
     5761 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac     
     5821 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc     
     5881 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg     
     5941 gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc     
     6001 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag     
     6061 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc     
     6121 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc     
     6181 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca acctctggat     
     6241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt     
     6301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc     
     6361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg     
     6421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc     
     6481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa     
     6541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat     
     6601 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc     
     6661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt     
     6721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga     
     6781 cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg ctaattcact     
     6841 cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct     
     6901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc     
     6961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc     
     7021 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta     
     7081 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg     
     7141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     
     7201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc     
     7261 atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt atgggtctgc     
     7321 attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta aggagaaaat     
     7381 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     
     8641 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg     
     8701 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa     
     8761 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg     
     8821 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg     
     8881 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc     
     8941 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg     
     9001 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg     
     9061 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa     
     9121 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct     
     9181 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga     
     9241 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc     
     9301 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac     
     9361 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc     
     9421 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact     
     9481 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga     
     9541 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg     
     9601 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga     
     9661 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga     
     9721 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt     
     9781 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat     
     9841 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat     
     9901 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac     
     9961 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc     
    10021 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga     
    10081 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     
    10141 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg     
    10201 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt                
//