pLenti-5-UTR-Luc

                            LOCUS       dna                     10227 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2439..2444
                     /dbxref="REBASE:ClaI"
     misc_binding    2463..2468
                     /dbxref="REBASE:KpnI"
     misc_binding    2490..2495
                     /dbxref="REBASE:EcoRI"
     ORF             2915..3586
                     /sequence="ORF_6 rf(6)"
     misc_binding    3534..3539
                     /dbxref="REBASE:EcoRV"
     misc_binding    3555..3560
                     /dbxref="REBASE:BamHI"
     ORF             3567..5219
                     /sequence="ORF_3 rf(3)"
     ORF             4563..5336
                     /sequence="ORF_5 rf(5)"
     Promoter        5288..5556
                     /gene="SV40 prom"
     Rep_Origin      5455..5532
                     /gene="SV40 origin"
     ORF             5522..6190
                     /sequence="ORF_2 rf(2)"
     misc_binding    5535..5547
                     /dbxref="REBASE:SfiI"
     Marker          5591..6190
                     /gene="puro marker"
     Other Gene      6800..6852
                     /gene="delta_U3 other"
     Other Gene      6853..7033
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7539..8158
                     /gene="pBR322 origin"
     Promoter        8811..8860
                     /gene="NEOKAN prom"
     ORF             8949..9743
                     /sequence="ORF_4 rf(3)"
     Marker          8952..9740
                     /gene="NTP_II marker"
     Rep_Origin      9848..10153
                     /gene="f1 origin"
BASE COUNT    2472 a   2727 c   2824 g   2204 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg agtacttacg     
     2461 taggtacccc agtgtggtgg cctgcaggtg aattcatagc agacatacaa cggacggtgg     
     2521 gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag gtcacccact     
     2581 aacgccccag gccttgtctt ggctgggcgt gactgttacc ctcaaaagca ggcagctcca     
     2641 gggtaaaagg tgccctgccc tgtagagccc accttccttc ccagggctgc ggctgggtag     
     2701 gtttgtagcc ttcatcacgg gccacctcca gccactggac cgctggcccc tgccctgtcc     
     2761 tggggagtgt ggtcctgcga cttctaagtg gccgcaagcc acctgactcc cccaacacca     
     2821 cactctacct ctcaagccca ggtctctccc tagtgaccca cccagcacat ttagctagct     
     2881 gagccccaca gccagaggtc ctcaggccct gctttcaggg cagttgctct gaagtcggca     
     2941 agggggagtg actgcctggc cactccatgc cctccaagag ctccttctgc aggagcgtac     
     3001 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc     
     3061 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag     
     3121 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt ggggcgcggg     
     3181 ggccacggcg cgcgccccca gcccccgggc ccagcacccc aaggcggcca acgccaaaac     
     3241 tctccctcct cctcttcctc aatctcgctc tcgctctttt tttttttcgc aaaaggaggg     
     3301 gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg gtgagtgagc ggcgcggggc     
     3361 caatcagcgt gcgccgttcc gaaagttgcc ttttatggct cgagcggccg cggcggcgcc     
     3421 ctataaaacc cagcggcgcg acgcgccacc accgccgaga ccgcgtccgc cccgagatct     
     3481 cagagcctcg cctttcatat cggcgcgcca ctagtaccgg taggcctgtc gacgatatcg     
     3541 ggcccgcggc cgctggatcc gccaccatgg aagatgccaa aaacattaag aagggcccag     
     3601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc     
     3661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta     
     3721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc     
     3781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg     
     3841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc     
     3901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag     
     3961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca     
     4021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt     
     4081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca     
     4141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc     
     4201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca     
     4261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca     
     4321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc     
     4381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat     
     4441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga     
     4501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc     
     4561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca     
     4621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta     
     4681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg     
     4741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca     
     4801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact     
     4861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag     
     4921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc     
     4981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa     
     5041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc     
     5101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg     
     5161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaac     
     5221 tcgagcctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa     
     5281 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat     
     5341 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg     
     5401 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg     
     5461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt     
     5521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt     
     5581 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc     
     5641 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt     
     5701 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt     
     5761 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac     
     5821 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga     
     5881 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg     
     5941 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa     
     6001 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc     
     6061 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac     
     6121 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc     
     6181 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac     
     6241 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt     
     6301 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt     
     6361 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt     
     6421 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg     
     6481 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg     
     6541 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct     
     6601 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt     
     6661 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc     
     6721 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc     
     6781 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt     
     6841 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa     
     6901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt     
     6961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg     
     7021 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag     
     7081 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa     
     7141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt     
     7201 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag     
     7261 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc     
     7321 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct     
     7381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac     
     7441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga     
     7501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat     
     7561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac     
     7621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     
     7681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     
     7741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     
     7801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     
     7861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     
     7921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     
     7981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga     
     8041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt     
     8101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt     
     8161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga     
     8221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc     
     8281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct     
     8341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata     
     8401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca     
     8461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga     
     8521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga     
     8581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga     
     8641 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct     
     8701 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg     
     8761 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc     
     8821 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct     
     8881 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat     
     8941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga     
     9001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc     
     9061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga     
     9121 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg     
     9181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc     
     9241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg     
     9301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga     
     9361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc     
     9421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca     
     9481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg     
     9541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct     
     9601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg     
     9661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc     
     9721 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt     
     9781 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg     
     9841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc     
     9901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca     
     9961 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga     
    10021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa     
    10081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc     
    10141 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct     
    10201 taattaacat catcaataat atacctt                                         
//