Question (1072)
Question
Can I insert my own polyA signal into abm’s lentiviral vector? Where is the polyA signal located in abm’s lentiviral vectors?
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Since lentivirus is an RNA virus, during the synthesis of the RNA genome to be packaged, if there is a polyadenylation signal in the insert, the RNA will be terminated prematurely. There is a SV40 poly(A) signal in the vector located downstream of the 3'LTR. It is designed this way to ensure a high amount of transcriptional RNA is present so that a high viral titer is obtained. We have received positive feedback from previous customers in regards to the high and stable expression levels of our lentivectors.
ABM Scientific Support
Answered on Mar 24 2025