qPCR Lentivirus Titer Kit
Cat. No.
LV900
Unit
100 rxn
Price
$212.00
| Cat. No. | LV900 | |||||||||||||||||||
| Name | qPCR Lentivirus Titer Kit | |||||||||||||||||||
| Unit | 100 rxn | |||||||||||||||||||
| Category | qPCR Virus Titer Kits | |||||||||||||||||||
| Description |
The only kit on the market with complete elimination of NTC! abm’s qPCR Lentivirus Titer Kit is a one-step assay that employs a quick RNA extraction step that is followed by RT-qPCR, offering high sensitivity and specificity for accurate lentivirus titer quantification. Designed to minimize non-specific background, this kit delivers superior performance compared to others on the market. The MasterMix contains a dye similar to SYBR Green™ and EvaGreen®, optimizing amplification efficiency, while the ROX Reference Dye is provided separately for broad compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our ROX Machine Compatibility Chart. Kit Features:
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| Storage Condition |
Store at -20°C. This product is stable for 2 year from the date of shipping if stored and handled properly. |
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| Note |
qPCR Lentivirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV900 |
| Why do I get higher titers for the more diluted sample from the same stock? | |
|
This means your virus stock may have strong PCR inhibitors in the sample. Thus, it would best to trust the more diluted samples' results, as it lessens the inhibitor's influence.
|
| Will the transfected plasmids used to produce the lentiviral particles interfere with the qPCR results? What about the host's genome? | |
|
The transfection plasmids and host's genome remain in the packaging cells while the viral particles are harvested from the supernatant. The titering process will not involve the cells, thus the plasmids and genome will not interfere with the results as well.
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| Can this kit titer FIV particles? | |
|
No, this kit cannot titer FIV particles.
|
| My experiment needs MOI=10. How can I know that viral particle? How to IU(infectious units)/ml convert to viral particle? | |
|
Multiplicity of Infection (MOI) Calculation Method: MOI = (Product Titer (IU/ml) x Virus Volume (ml)) /Total Cell Number For example, to achieve a MOI of 10 with a 1 x 10^6 IU/ml titer virus on 1 x 10^5cells, use 1.0 ml of the virus.
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| Does your titration kit work with pLKO.1 based siRNA lentivirus? | |
|
Our titer kit should be able to detect pLKO.1 vector with an qPCR product size of about 100 bp.
|
| Can I use this kit to exclude the generation of replication competent lentivirus (RCL) in previously infected cells (stable cell lines?) so I can safely transfer them to Biosafety Level 1? | |
|
Yes, this kit can be used to determine that no replicative competent virus is being produced by previously infected cells. It is important to consult with the health and safety officer(s) at your own institution in order to determine if infected cells can be transferred to Biosafety Level 1.
|
| Is this kit suitable for titrating retroviruses and adenoviruses? | |
|
No, this kit is designed for Lentiviruses only.
|
| The dissociation curve shows that the Tm of my Lentivirus is lower than that of standards, why? | |
|
The Tm of a qPCR product is mainly depending on two variables: the reaction mixture's chemical compositions and the sequence of the amplicon. Since all the reactions in this case have almost the same compositions (ex: all the input templates are basically water, not for instant high salt template solution), the main variation would be in the template sequence. It is possible that your the vector has a slightly different sequence (likely having less GC content) than our std's sequence, thus having a lower Tm than the Std.
|
| Will the qPCR Lentivirus Titration(Titer) Kit amplify the integrated genomic region of the lentivirus? | |
|
No, the qPCR Lentivirus Titration (Titer) Kit (Cat#LV900) will not amplify the integrated genomic regions of lentivirus because the primers amplify the 5' LTR of the lentivirus.
|
| Does the assay detect free viral RNA and so overestimate the viral titer? If so, is there a way to minimize this? | |
|
In theory, it is possible for our Cat#LV900 to detect free viral RNA and free viral RNA can be present in the supernatant collected during lentivirus production, however this should not impact the titer to any significant extent and should be very minimal in detection, if any. If desired, you may wish to further purify your virus using our Ultra-Pure Lentivirus Purification Kit (Cat#LV998) or Speedy Lentivirus Purification (Cat#LV999) to remove any free viral RNA. However, we have never encountered any issues using our LV900 kit and free viral RNA has not been a factor; we use this kit regularly during our routine QC processes, and it is one of the most popular products in our catalogue.
|
| For lenti titering, is it possible to start with an ultracentrifuged lenti sample (suspended in PBS) as opposed to straight supernatant? | |
|
Yes, starting with an ultracentrifuged lenti sample should indeed be suitable for use with our qPCR Lentivirus Titration(Titer) Kit (LV900).
|
| Is this kit suitable for detecting both 2nd and 3rd generation lentivirus? | |
|
Yes, the kit is suitable for detecting both 2nd and 3rd generation lentivirus.
|
| What type of vectors will this kit work with? | |
|
The titer primers supplied in LV900 are designed for all HIV-1 based viral detection.
|
| Is DNAse treatment required after harvesting the lentivirus stock? | |
|
DNase treatment is not recommended. When packaging lentivirus, we recommend removing the transfection medium from the cells after overnight incubation, and replace with complete culture media for the cells. Incubate another 24 hours, and then collect the supernatant.
|
| What is the primer design based on? | |
|
The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
|
| How come my cells are not successfully infected with the evaluated titer from the kit? | |
|
One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
|
| Does the standard need to be diluted each time? Can diluted standards be used multiple times? | |
|
It is advised to dilute the standard fresh each time as the diluted standards might be degraded over time, thus compromising their accuracy.
|
| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
|
| How much ROX reference dye should I add to the MasterMix? | |
|
The recommended volume of ROX reference dye varies based on the qPCR instrument:
|
| Can I use this MasterMix with any qPCR machine? | |
|
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-adeno-associated-virus-titration-titer-kit-g931.html#g931\u0022\u003EqPCR AAV Titer Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-adeno-associated-virus-titration-titer-kit-g931.html#g931\u0022\u003EG931\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G931\u0022\u003E$212.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-lentivirus-titration-titer-kit.html\u0022\u003EqPCR Lentivirus Titer Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-lentivirus-titration-titer-kit.html\u0022\u003ELV900\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022LV900\u0022\u003E$212.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-retrovirus-titration-titer-kit-g949.html#G949\u0022\u003EqPCR Retrovirus Titer Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/qpcr-retrovirus-titration-titer-kit-g949.html#G949\u0022\u003EG949\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G949\u0022\u003E$212.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResults in only 1 hour\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/viral-titer-kits-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003Eabm\u0026rsquo;s qPCR Lentivirus Titer Kit provides results in only 1 hour. \u003C\/strong\u003E Total qRT-PCR reaction times of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s qPCR Lentivirus Titer Kit (Cat. No. LV900), Taqman\u0026reg; RNA-to-Ct\u0026trade; 1-Step Kit, and time required for a typical 2-step reaction were compared.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EAccurate Titers\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/viral-titer-kits-data-2.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003Eabm\u0026rsquo;s qPCR AAV Titer Kit provides consistent, and accurate titer measurements. \u003C\/strong\u003E The titers of AAV3, 5, and 7 were measured using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s qPCR AAV Titer Kit (Cat. No. G931) containing primers for both CMV and ITR. The titer results for each serotype show there is no significant difference in real-time PCR amplification for CMV or ITR.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EEasy Visualization\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/qPCR-lenti-titer-kit-graph.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003Eabm\u0026rsquo;s qPCR Lentivirus Titer Kit enables easy visualization of viral titers.\u003C\/strong\u003E A lentivirus sample (blue) was processed following the protocol for the qPCR Lentivirus Titer Kit (Cat. No. LV900) and amplified in a qPCR reaction along with standards included in the kit (purple).\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003C\/p\u003E\n\u003Ch5 class=\u0022abm-custom-page-p\u0022\u003E\u0026nbsp;\u003C\/h5\u003E\n\u003Cdiv class=\u0022abm-categories-text\u0022\u003E\n\u003Ctable border=\u00220\u0022 cellpadding=\u002210\u0022 style=\u0022background-color: #f9eae4; border-radius: 10px; vertical-align: middle; width: 99.1458%;\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022width: 5.2856%;\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/promotion-tag-v2.png\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 91.6725%;\u0022\u003E\n\u003Cdiv class=\u0022abm-categories-text\u0022\u003E\u003Cstrong\u003EBundle up, save more!\u0026nbsp;\u003C\/strong\u003E\u0026nbsp;These\u0026nbsp;\u003Ca href=\u0022\/Lentivirus-Bundles.html\u0022 title=\u0022Lentivirus Bundles\u0022\u003Ecost-saving bundles\u003C\/a\u003E\u0026nbsp;combine our best-selling Lentivirus qPCR titer kit with reagents for virus packaging and transduction!\u003C\/div\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-categories-text\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-categories-text\u0022\u003E\u003Cstrong\u003E\u003C\/strong\u003E\u003Cspan style=\u0022color: #ffffff;\u0022\u003E.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12 col-md-12 abm-custom-page-padding-left-zero\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12 col-md-12 col-lg-12 abm-custom-page-padding-left-zero\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESimply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1600px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E \u003C!-- onload=\u0022window.parent.scrollTo(0,0)\u0022--\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResources\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-introduction\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/Resources_DNA-Polymerase-Variations.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to PCR\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"qPCR Virus Titer Kits","meta_keywords":"qPCR Virus Titer Kits","meta_description":"qPCR Virus Titer Kits","deleted_at":null,"enable":"Y","parent_list":"8","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":747,"cat_no_base":"LV900","parent_id":82,"description":"\u003Cp\u003E\u003Cstrong\u003EThe only kit on the market with complete elimination of NTC!\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s \u003Cstrong\u003EqPCR Lentivirus Titer Kit \u003C\/strong\u003Eis a one-step assay that \u003Cstrong\u003Eemploys a quick RNA extraction step that is followed by RT-qPCR\u003C\/strong\u003E, offering \u003Cstrong\u003Ehigh sensitivity and specificity \u003C\/strong\u003Efor accurate lentivirus titer quantification. Designed to minimize non-specific background, this kit delivers superior performance compared to others on the market. The \u003Cstrong\u003EMasterMix\u003C\/strong\u003E contains a dye similar to SYBR Green\u0026trade; and EvaGreen\u0026reg;, optimizing amplification efficiency, while the \u003Cstrong\u003EROX Reference Dye\u003C\/strong\u003E is provided separately for broad compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our \u003Ca href=\u0022\/uploads\/document\/ROX%20Machine%20Compatibility%20List_v1.pdf\u0022\u003EROX Machine Compatibility Chart\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EKit Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003ESaves time and eliminates inaccuracy: \u003C\/strong\u003EQuick\u0026nbsp;RNA extraction\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ENo NTC amplification:\u003C\/strong\u003E Eliminates non-specific (NTC) signals due to our unique primer design\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ECan be used directly on neat viral particles\u003C\/strong\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EReduces variability:\u003C\/strong\u003E Ready-to-use reagent mix\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ERT-qPCR in one step:\u003C\/strong\u003E More sensitive and accurate than other methods\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EContains dye comparable to SYBR Green\u0026trade; and EvaGreen\u0026trade;\u003C\/strong\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; vertical-align: middle; margin: 20px 0px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr valign=\u0022middle\u0022\u003E\n\u003Ctd\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/promotion-tag-v2.png\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cp\u003E\u003Cstrong\u003EBundle up, save more!\u0026nbsp;\u003C\/strong\u003E\u0026nbsp;These\u0026nbsp;\u003Ca title=\u0022Lentivirus Bundles\u0022 href=\u0022\/Lentivirus-Bundles.html\u0022\u003Ecost-saving bundles\u003C\/a\u003E combine our best-selling Lentivirus qPCR Titer Kit with reagents for virus packaging and transduction!\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EBlasTaq\u0026trade; 2X qPCR Titer MasterMix\u003C\/td\u003E\n\u003Ctd\u003E1.25 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EPrimer Mix\u003C\/td\u003E\n\u003Ctd\u003E100 rxn (200 \u0026micro;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EStandard Control DNA\u003C\/td\u003E\n\u003Ctd\u003E50 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EVirus Lysis Buffer\u003C\/td\u003E\n\u003Ctd\u003E800 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EROX Reference Dye\u003C\/td\u003E\n\u003Ctd\u003E15 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENuclease-Free H\u003Csub\u003E2\u003C\/sub\u003EO\u003C\/td\u003E\n\u003Ctd\u003E2 x 1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #d6effa; border-radius: 10px; vertical-align: middle; margin-bottom: 20px;\u0022 border=\u00220\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022padding: 30px;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003ECustomer Testimonials\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0022I recently received one of your qPCR Lentivirus Titration Kits and I think it is a wonderful product. The protocol is simple, fast, and accurate. I also really appreciate that there is an online titer calculator accompanying the kit on your website. The kit is allowing me to produce my own lentivirus, which is cheaper and more customizable.\u0022\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003E- Kelly Taylor, Medical University of South Carolina\u003C\/em\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0022We like the qPCR Lentivirus Titration(Titer) Kit. It is very easy to use and the titration can be completed in a short time.\u0022\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003E- Ying Liu, University of Texas Health Science Center Houston\u003C\/em\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0022We have been using Lentivirus qPCR kit to check our viruses for those viruses without reporter genes for a few years. The kit is very easy to use. We love it.\u0022\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003E- Xinping Huang, Emory University\u003C\/em\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0022I tested LV900 with the dilution that you suggested and also compared it to another method that measures the functional titer counting the viral integrations in the genome of HEK cells. Diluting the viral lysate solved my problems and improved my results. Now, I decided to use the kit for my future lentiviral titration. Thank you very much for your help and the great service!\u0022\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003E- Anna Schnaubelt,\u0026nbsp;University Hospital Heidelberg\u003C\/em\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022vertical-align: middle;\u0022\u003E\n\u003Ctd style=\u0022text-align: left; vertical-align: middle;\u0022 width=\u002215%\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\u0022 alt=\u0022ISO 13485:2016 MDSAP Certified\u0022 width=\u002285%\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003EISO 13485:2016 MDSAP Certified\u003C\/strong\u003E\u003Cbr \/\u003EOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"no_selection","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp\u003EStore at -20\u0026deg;C.\u0026nbsp;This product is stable for 2 year from the date of shipping if stored and handled properly.\u0026nbsp;\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":"PCR Team","stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"PCR-1B","note":"\u003Cp\u003EqPCR Lentivirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\n\u003Cdiv\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EMACHINE TYPE\u003C\/th\u003E\n\u003Cth\u003EMACHINE\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EHigh ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026nbsp;\u0026reg; 7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u0026trade;, StepOne\u0026trade;, OpenArray, PRISM\u0026trade; Sequencing Detection Series\u003C\/li\u003E\n\u003Cli\u003EBiometra TOptical\u003C\/li\u003E\n\u003Cli\u003EFluidigm BioMark\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EWafergene SmartChip System\u003C\/li\u003E\n\u003Cli\u003ETianLong TL998 System\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ELow ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026reg; 7500, 7500 Fast, Viia\u0026trade; 7, QuantStudio, QuantStudio 3\/5\/6\/7\u003C\/li\u003E\n\u003Cli\u003EBioGene InSyte\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EIllumina Eco\u003C\/li\u003E\n\u003Cli\u003EAnalytikjena qTower Series\u003C\/li\u003E\n\u003Cli\u003EStratagene\u0026reg; Mx3000, Mx3005, Mx4000\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENo ROX machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EBioRad\u0026reg; CFX96, CFX384, Chromo4\u0026trade;, CFX Connect\u0026trade;, Opticon 2, MiniOpticon\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ERoche LightCycler\u0026reg; (480, 1536, Nano)\u003C\/li\u003E\n\u003Cli\u003EMJ Research Opticon\u0026trade;, Opticon\u0026trade; 2, Chromo\u0026reg; 4\u003C\/li\u003E\n\u003Cli\u003EEppendorf\u0026trade; Realplex 4\u003C\/li\u003E\n\u003Cli\u003EBioGene SynChron\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ECorbett Rotor-gene\u0026reg; (3000, 6200, 62H0, 6500, 65H0, 6600)\u003C\/li\u003E\n\u003Cli\u003EEppendorf Mastercycler\u0026reg; realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)\u003C\/li\u003E\n\u003Cli\u003ECepheid SmartCyler, GeneXpert\u003C\/li\u003E\n\u003Cli\u003EEnigma\u0026trade; ML\u003C\/li\u003E\n\u003Cli\u003EIdaho LightScanner\u0026reg; (24, 32), RapidCycler\u0026reg;2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS\u003C\/li\u003E\n\u003Cli\u003EQiagen Rotor-Gene\u0026trade; (Q, 6000)\u003C\/li\u003E\n\u003Cli\u003ETakara Dice\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EThermo Scientific PikoReal\u003C\/li\u003E\n\u003Cli\u003EDNA-Technology DT96, DTlite, DT-322\u003C\/li\u003E\n\u003Cli\u003EBioer LineGene (3310\/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)\u003C\/li\u003E\n\u003Cli\u003EBioneer Exicycler\u0026trade;\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":null,"updated_at":"2025-03-26 22:19:24","short_description":"\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u2019s\u00a0qPCR Lentivirus Titer Kit\u00a0is a one-step assay which does not require additional lysis or reverse transcription (RT) steps, and can be completed under one hour.\u00a0\u003C\/p\u003E","reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379815,"parent_id":82,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/l\/v\/lv900-lentivirus-titratinon-kit.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18085082,"sku_in_m2":"LV900","value_id_m2":49912430,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:39:19"},{"id":379817,"parent_id":82,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/l\/v\/lv900-qpcr-lentivirus-titer-kit_data2.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":18085082,"sku_in_m2":"LV900","value_id_m2":49912332,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2025-03-25 17:24:29"},{"id":388624,"parent_id":82,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/WV5YUHF8Z1qhZfTOiOrO9PrmTYTI3n3pEJTIDYfW.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-25 17:24:25","updated_at":"2025-03-25 17:24:29"}],"gene":null}