Safe-Green™
Cat. No.
G108-G
Unit
1.0 ml
Price
$47.00
| Cat. No. | G108-G |
| Name | Safe-Green™ |
| Unit | 1.0 ml |
| Category | Gel Documentation |
| Description |
Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis. Features:
|
| Safeview Series | Loading Dye |
| LED Viewer Compatibility | Yes |
| Stain Color | Green |
| Application |
Safe detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels. |
| Shipping Conditions |
Shipped on blue ice packs. |
| Storage Condition |
Store at 4°C for up to 2 years. |
| Note |
Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108-G |
Datasheet
Search CoA here
MSDS
| Can SafeView products be used in replacement of ethidium bromide? | |
|
All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow. |
| How do SafeView products work and why are they not carcinogenic? | |
|
SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide. |
| How do I use SafeView products? | |
|
Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel. |
| Can SafeView products differentiate between double stranded and single stranded nucleic acids? | |
|
No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.
|
| At what temperature do I store SafeView products? | |
|
SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C. |
| How sensitive are SafeView products? | |
|
Safe-Green™: 0.2-0.6 ng DNA per band |
| Can SafeView products be used to post-stain gels? | |
|
Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.
|
| What is the functional pH range for Safe-Green? | |
|
Safe-Green™ functions optimally between pH 7 to 9.
|
| Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel? | |
|
If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis. |
| We notice band shifting of our DNA fragments when using Safe-Green. Are there any recommendations that you can give us to minimize this band shifting? | |
|
Band shifting to a certain degree is unavoidable for any nucleic acid stain as most of these fluorescent compounds are large, positively charged molecules. Loading dye format nucleic acid stains, while convenient, may also suffer from more prominent band shifting due to the stain binding and migrating with the sample simultaneously during electrophoresis. Post-staining, while takes longer to complete, may be the optimal choice of nucleic acid stain strategy if band shifting issue is a priority. We have observed band shifting with Safe-Green™ as this issue has been documented in the literature to be an inherent property of the specific fluorescent compound. Alternatively we offer SafeView™ Classic which we have observed to exhibit no band shifting issues. |
| I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do? | |
|
It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.
|
| Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel? | |
|
SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.
|
| Can SafeView products be stored at -20°C? | |
|
No, please store Safe-Green™ at 4°C and SafeView™ Classic, Safe-Red™ and Safe-Red™ Gel at 18-25°C. |
| What is the concentration of SafeView products? | |
|
SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.
|
| Would adding Safe-Green to the DNA ladder interfere with the migration rate of the loading dye of the ladder? | |
|
No, adding Safe-Green™ to the DNA ladder will not interfere with the DNA ladder's loading dye. You still need to add Safe-Green™ to the DNA ladder at the same ratio. To be more technically correct, Safe-Green™, and all other EB alternative DNA binding dyes, alter the migration pattern of DNA on agarose gel by shifting DNA to a larger molecular size; it is just simply because the overall molecular size of a particular DNA band increases as more dye is binding onto it. This is why we recommend mixing the DNA ladder and Safe-Green™ at the exact same 1:6 ratio, so that all samples on the gel will be subjected to the same concentration of Safe-Green™ and thus have the same shift.
|
| Can Safe-Green penetrate the nucleus? | |
|
Yes, our Safe-Green™ stain indeed has the ability to penetrate the nucleus.
|
| What color is the tracking dye of Safe-Green and Safe-Red during electrophoresis? | |
|
Safe-Green™ and Safe-Red™ both contain a dark blue tracking dye that migrates at ~300bp on a 1% agarose gel. Safe-Red™ contains an additional light blue tracking dye that migrates at ~4000bp on a 1% agarose gel.
|
| Can I perform gel extraction and subsequent cloning using SafeView products? | |
|
We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.
|
| What type of device or imaging system should be used to visualize SafeView agarose gels? | |
|
We recommend using abm’s SafeViewER™ Imager (Cat.No E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.
|
- Ibeagha-Awemu, EM et al. "Proteomics, genomics, and pathway analyses of Escherichia coli and Staphylococcus aureus infected milk whey reveal molecular pathways and networks involved in mastitis" J. Proteome Res. 9 (9):4604-4619 (2010). DOI: 10.1021/pr100336e. PubMed: 20704270. Application: PCR Products Viewing.
- Diaz-Balzac, CA et al. "Calbindin-D32k is localized to a subpopulation of neurons in the nervous system of the sea cucumber Holothuria glaberrima (Echinodermata)" PLoS ONE 7 (3):e32689 (2012). DOI: 10.1371/journal.pone.0032689. PubMed: 22412907. Application: PCR Products Viewing.
- Machida, RJ et al. "PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences" PLoS ONE 7 (9):e46180 (2012). DOI: 10.1371/journal.pone.0046180. PubMed: 23049971. Application: PCR Products Viewing.
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- Goo, JS et al. "Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain" Carbohydr. Res. 07-Dec:386 (2014). DOI: 10.1016/j.carres.2013.12.005. PubMed: 24456970. Application: PCR Products Viewing.
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- Ish-Shalom, S et al. "Analysis of fungal gene expression by Real Time quantitative PCR" Methods Mol. Biol. 638:103-114 (2010). DOI: 10.1007/978-1-60761-611-5_7. PubMed: 20238263. Application: PCR Products Viewing.
- Lam, SW et al. "Rapid, specific and quantitative polymerase chain reaction (PCR) detection of pathogenic protozoa Entamoeba histolytica for drinking water supply" Water Science & Technology: Water Supply 11 (4):418-425 (2011). DOI: 10.2166/ws.2011.057. PubMed: 67657423. Application: PCR Products Viewing.
- Chang, KF et al. "First report of Fusarium proliferatum causing root rot in soybean (Glycine max L.) in Canada" Crop Prot 67:52-58 (2015). DOI: 10.1016/j.cropro.2014.09.020. Application: PCR Products Viewing.
- Mun, Y. S., & Hwang, Y. J. "Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean" Antibiotics 8(4):202 (2019). DOI: 10.3390/antibiotics8040202.
- Dort, E. N., & Hamelin, R. C. (2024). Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthoraspecies. https://doi.org/10.1101/2024.06.13.598956
- Helmerich, D. A., Budiarta, M., Taban, D., Doose, S., Beliu, G., & Sauer, M. (2023). PCNA as Protein‐Based Nanoruler for Sub‐10 nm Fluorescence Imaging. Advanced Materials, 36(7). Portico. https://doi.org/10.1002/adma.202310104
- Mahanta, A. K., Chaulagain, B., Trivedi, R., & Singh, J. (2024). Mannose-Functionalized Chitosan-Coated PLGA Nanoparticles for Brain-Targeted Codelivery of CBD and BDNF for the Treatment of Alzheimer’s Disease. ACS Chemical Neuroscience, 15(21), 4021–4032. https://doi.org/10.1021/acschemneuro.4c00392
- Noseworthy, M. K., Allen, E. A., Dale, A. L., Leal, I., John, E. P., Souque, T. J., Tanney, J. B., & Uzunovic, A. (2024). Evidence to support phytosanitary policies–the minimum effective heat treatment parameters for pathogens associated with forest products. Frontiers in Forests and Global Change, 7. https://doi.org/10.3389/ffgc.2024.1380040
- Thanwiset, T., Pitaksakulrat, O., Hongsrichan, N., Boonmars, T., Bunchu, N., Thipphet, K., Chaisongkram, C., Ponsrila, K., Kimkamkaew, S., Rompo, T., Kwak, M. L., Nakao, R., Blair, D., & Eamudomkarn, C. (2024). First report of Lipoptena axis Maa, 1965, from captive cervids in Thailand, based on morphological and molecular data. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-81179-3
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width: 12.9599%;\u0022\u003E\u2714\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: center; width: 26.1517%;\u0022\u003E1.0 \u0026ndash; 2.0 ng\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.80657%;\u0022\u003E1.0 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G108\u0022 style=\u0022width: 6.94575%;\u0022\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ESafeView\u0026trade; DNA Stains\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12 col-md-8\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESafeView\u0026trade; products are a new class of fluorescent nucleic acid stains for the detection of double-stranded DNA, single-stranded DNA and RNA in agarose gel electrophoresis. Four formulations are available, including green or red fluorescence and gel-casting or loading dye formats, making them compatible for any experimental preference or setup!\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1.2em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/safeview-icon-safe.png\u0022 \/\u003E\n\u003Cp\u003ESafe\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/safeview-icon-easy-to-use.png\u0022 \/\u003E\n\u003Cp\u003EEasy-to-use\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/safeview-icon-sensitive.png\u0022 \/\u003E\n\u003Cp\u003ESensitive\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EIncreased sensitivity compared to ethidium bromide\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1.2em;\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/zfDIxlj3gy2eopP3i3Wi9R2WdP8H2Ak7B99kWBnL.png\u0022 alt=\u0022\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003ESafeView\u0026trade; Classic has increased sensitivity compared to ethidium bromide.\u003C\/strong\u003E\u0026nbsp;Two-fold serial dilution of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s 1kb Plus Opti-DNA Marker (Cat. 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No. G248) was visualized using SafeView\u0026trade; Classic on a 1% agarose gel in TAE Buffer with the SafeViewER\u0026trade; Imager.\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EMeet the new SafeViewER\u0026trade; Imager\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1.2em;\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cb\u003ESafer by design:\u0026nbsp;\u003C\/b\u003EReplaces EtBr with non-carcinogenic SafeView\u0026trade; dyes\u003C\/li\u003E\n\u003Cli\u003E\u003Cb\u003EDouble protection:\u0026nbsp;\u003C\/b\u003EEliminates UV exposure\u003C\/li\u003E\n\u003Cli\u003E\u003Cb\u003EEnhanced sensitivity:\u0026nbsp;\u003C\/b\u003ELowers limit of detection to 0.06 ng\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/SafeViewER-Imager.html\u0022\u003EClick here\u003C\/a\u003E to learn more about the SafeViewER\u0026trade; Imager!\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/ROc8CEoXqZjS1xoE8NTWK1TuFaCm6e1QqIKXuEzF.png\u0022 alt=\u0022\u0022 \/\u003E\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-vectors-img col-sm-12 col-md-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/pcr\/safeview-non-toxic-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022 abm-page-left-link\u0022 data-text=\u0022Request a Free Sample\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESimply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. 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This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EFeatures:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003EConvenient:\u003C\/strong\u003E Safe-Green\u0026trade; is provided as a 6X loading dye, and is mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EEasy to Use:\u003C\/strong\u003E View and document your results as you would with EtBr staining. Safe-Green\u0026trade; can be excited with blue or UV light, and has maximum emission at 525 nm.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ESafe:\u003C\/strong\u003E Non-carcinogenic.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ESensitive:\u003C\/strong\u003E Detect as little as 0.2 \u0026ndash; 0.6 ng of DNA per gel band.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ESuperior:\u003C\/strong\u003E EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Green\u0026trade; minimizes such damage, yielding higher transformation rates and lower mutation rates verses EtBr. 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