PL-SIN-EOS-S(4)-EiP
| Vector Name | PL-SIN-EOS-S(4)-EiP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gcggccgctg gaagggctaa
421 tttggtccca aaaaagacaa gagatccttg atctgtggat ctaccacaca caaggctact
481 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
541 ggtgcttcaa gttagtacca gttgaaccag agcaagtaga agaggccaaa taaggagaga
601 agaacagctt gttacaccct atgagccagc atgggatgga ggacccggag ggagaagtat
661 tagtgtggaa gtttgacagc ctcctagcat ttcgtcacat ggcccgagag ctgcatccgg
721 agtactacaa agactgctga catcgagctt tctacaaggg actttccgct ggggactttc
781 cagggaggtg tggcctgggc gggactgggg agtggcgagc cctcagatgc tacatataag
841 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
901 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa
961 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
1021 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc
1081 cagaggagat ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
1141 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
1201 ggtgcgagag cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta
1261 aggccagggg gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta
1321 gaacgattcg cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg
1381 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
1441 atagcagtcc tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta
1501 gataagatag aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac
1561 acaggaaaca acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa
1621 atggtacatc aggccatatc acctagaact ttaaattaag acagcagtac aaatggcagt
1681 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
1741 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
1801 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggaaag gaccagcaaa
1861 gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt
1921 gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa cagatggcag gtgatgattg
1981 tgtggcaagt agacaggatg aggattaaca catggaaaag attagtaaaa caccatagct
2041 ctagagcgat ccgatcttca gacctggagg aggcgatatg agggacaatt ggagaagtga
2101 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
2161 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
2221 cttgggagca gcaggaagca ctatgggctg cacgtcaatg acgctgacgg tacaggccag
2281 acaattattg tctgatatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
2341 acagcatctg ttgcaactca cagtctgggg catcaaacag ctccaggcaa gaatcctggc
2401 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
2461 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
2521 ttggaataac atgacctgga tggagtggga cagagaaatt aacaattaca caagcttggt
2581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
2641 accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc ccgaaggaat
2701 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctc
2761 cctttatcca gccctcactc cttctctagg cgccggaatt cccagtccaa gctaggcagg
2821 ttcccctcta attaatgcag agactctaaa agaatttccc gggctcgggc agccattgtg
2881 atgcatatag gattattcac gtggtaatga gcacagtcga cagttcttgc tctcgagtag
2941 aatcgaattc ccagtccaag ctaggcaggt tcccctctaa ttaatgcaga gactctaaaa
3001 gaatttcccg ggctcgggca gccattgtga tgcatatagg attattcacg tggtaatgag
3061 cacagtcgac agttcttgct ctcgagtaga atcgaattcc cagtccaagc taggcaggtt
3121 cccctctaat taatgcagag actctaaaag aatttcccgg gctcgggcag ccattgtgat
3181 gcatatagga ttattcacgt ggtaatgagc acagtcgaca gttcttgctc tcgagtagaa
3241 tcgaattccc agtccaagct aggcaggttc ccctctaatt aatgcagaga ctctaaaaga
3301 atttcccggg ctcgggcagc cattgtgatg catataggat tattcacgtg gtaatgagca
3361 cagtcgacag ttcttgctct cgagtagaat cgaattctcc cctcccccag cctgaaacct
3421 gcttgctcag gggtggagct tcctgctcat tcgttctgcc acgcccactg ctggaacctg
3481 cggagccaca cccgtgcacc tttctactgg accagagatt attcggcggg aatcgggtcc
3541 cctccccctt ccttcataac tagtgtcgca actataaaat ttgagccttg atcagagtaa
3601 ctgtcttggc tacattcttt cttccgcccc gtctagattc ctctcttaca gctcgagcgg
3661 ccttctcagt cgaaccgttc acgttgcgag ctgctggcgg ccgcaacaag atctgcgatc
3721 taagtaagct tggcattccg gtactgttgg taaagccacc atggtgagca agggcgagga
3781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
3841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
3901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
3961 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
4021 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
4081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
4141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
4201 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
4261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
4321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
4381 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
4441 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccaac ctcgacgtta
4501 ttcccttcga tcgaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
4561 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
4621 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
4681 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
4741 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
4801 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
4861 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
4921 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
4981 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
5041 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
5101 cacaaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
5161 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
5221 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
5281 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
5341 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
5401 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
5461 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
5521 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
5581 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
5641 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
5701 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
5761 atcgatttcg aacccggggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
5821 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagacaa
5881 gatctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
5941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
6001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
6061 tcagtgtgga aaatctctag cagaattcta gcttggcgta atcatggtca tagctgttcc
6121 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
6181 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
6241 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
6301 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
6361 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
6421 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
6481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
6541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
6601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
6661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
6721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
6781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
6841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
6901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
6961 tatctgcgct ctgctgaagc cacttacctt cggaaaaaga gttggtagct cttgatccgg
7021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
7081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
7141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
7201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
7261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
7321 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
7381 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
7441 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
7501 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
7561 gcgcaacgtt gtgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
7621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
7681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
7741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
7801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
7861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
7921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
7981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
8041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
8101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
8161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
8221 ggggttccgc gcacatttcc ccgaaaagtg ccacctggga ctagcttttt gcaaaagcct
8281 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
8341 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
8401 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
8461 tgtagcttgc atgccgacat ggattattga ctagtcccta agaaaccatt attatcatga
8521 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
LOCUS dna 8562 bp
FEATURES Location/Qualifiers
misc_binding 183..188
/dbxref="REBASE:NdeI"
Reporter 239..398
/gene="lacZ_a reporter"
misc_binding 520..525
/dbxref="REBASE:EcoRV"
Other Gene 862..1042
/gene="HIV-1_5_LTR other"
Other Gene 1153..1197
/gene="HIV-1_psi_pack other"
ORF 2078..2815
/sequence="ORF_1 rf(2)"
Regulatory_Seq 2200..2433
/gene="RRE reg"
misc_binding 2753..2758
/dbxref="REBASE:BamHI"
misc_binding 3589..3594
/dbxref="REBASE:BclI"
Reporter 3761..4477
/gene="EGFP reporter"
ORF 3761..4480
/sequence="ORF_2 rf(2)"
Regulatory_Seq 4520..5104
/gene="IRES reg"
Regulatory_Seq 4596..5096
/gene="ires_emcv reg"
misc_binding 4634..4639
/dbxref="REBASE:ApaI"
ORF 5009..5758
/sequence="ORF_4 rf(6)"
ORF 5096..5707
/sequence="ORF_3 rf(2)"
Marker 5108..5707
/gene="puro marker"
misc_binding 5761..5766
/dbxref="REBASE:ClaI"
Other Gene 5849..5901
/gene="delta_U3 other"
Other Gene 5902..6082
/gene="HIV-1_5_LTR other"
Promoter 6155..6184
/gene="lac prom"
Rep_Origin 6493..7112
/gene="pBR322 origin"
Marker 7267..8126
/gene="amp marker"
Promoter 8168..8196
/gene="amp prom"
Promoter 8263..8462
/gene="SV40 prom"
misc_binding 8325..8337
/dbxref="REBASE:SfiI"
Rep_Origin 8340..8417
/gene="SV40 origin"
misc_binding 8469..8474
/dbxref="REBASE:SphI"