pAAV-G-CAGGS-hGH-amp

                            LOCUS dna 7046 bp 
FEATURES Location/Qualifiers
misc_binding 413..418
/dbxref="REBASE:NdeI"
Promoter 545..822
/gene="cBA prom"
ORF 962..1693
/sequence="ORF_3 rf(5)"
misc_binding 1019..1024
/dbxref="REBASE:ApaI"
misc_binding 1783..1788
/dbxref="REBASE:XbaI"
misc_binding 1892..1897
/dbxref="REBASE:SacI"
Regulatory_Seq 1973..2012
/gene="tetO reg"
Other Gene 2026..2150
/gene="attR1 other"
Other Gene 2050..2150
/gene="attR2 other"
Marker 2259..2918
/gene="CAT marker"
ORF 2259..2918
/sequence="ORF_2 rf(3)"
misc_binding 2472..2477
/dbxref="REBASE:EcoRI"
Other Gene 3260..3565
/gene="ccdB other"
Other Gene 3606..3725
/gene="attR1 other"
Other Gene 3606..3706
/gene="attR2 other"
misc_binding 3741..3746
/dbxref="REBASE:XhoI"
Terminator 3758..4234
/gene="hGH_PA term"
misc_binding 4070..4075
/dbxref="REBASE:SphI"
Rep_Origin 4489..4795
/gene="f1 origin"
Promoter 5296..5324
/gene="amp prom"
Marker 5366..6226
/gene="amp marker"
ORF 5366..6226
/sequence="ORF_1 rf(2)"
Rep_Origin 6381..7000
/gene="pBR322 origin"
BASE COUNT 1540 a 1793 c 1976 g 1737 t 0 others
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
121 aggggttcct gcggccgccc taggccggcc acgcgtggcg cgttgacatt gattattgac 
181 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
241 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
301 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
361 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
421 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
481 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
541 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 
601 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 
661 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag 
721 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc 
781 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt 
841 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 
901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 
961 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg 
1021 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc 
1081 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt 
1141 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 
1201 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt 
1261 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca 
1321 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg 
1381 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 
1441 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc 
1501 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc 
1561 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg 
1621 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg 
1681 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga 
1741 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac 
1801 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg 
1861 tctcatcatt ttggcaaaga attggcgcgc cgagctcgtt tagtgaaccg tcagatcgcc 
1921 tggagacgcc atccacgctg ttttgacctc catagaagaa ccgagtttaa actccctatc 
1981 agtgatagag atctccctat cagtgataga gagctagcga tatcaacaag tttgtacaaa 
2041 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat 
2101 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat 
2161 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt 
2221 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat 
2281 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 
2341 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 
2401 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 
2461 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg 
2521 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 
2581 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 
2641 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 
2701 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 
2761 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 
2821 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat 
2881 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa 
2941 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg 
3001 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac 
3061 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg 
3121 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa 
3181 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac 
3241 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta 
3301 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat 
3361 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt 
3421 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc 
3481 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat 
3541 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg 
3601 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca 
3661 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt 
3721 acaaagtggt tgatatctga ctcgagccaa tcttaagggg tggcatccct gtgacccctc 
3781 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat 
3841 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag 
3901 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta 
3961 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg 
4021 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac 
4081 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct 
4141 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt 
4201 acaggcgtga accactgctc ccttccctgt ccttcacgtg cggaccgagc ggccgcagga 
4261 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 
4321 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 
4381 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 
4441 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 
4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct 
4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
4681 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
4801 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg 
4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 
4921 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc 
4981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 
5041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 
5101 ccgaaacgcg cgagccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 
5161 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 
5221 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
5281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
5341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
5401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
5461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
5521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
5581 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
5641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
5701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
5761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
5821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
5881 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
5941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
6001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
6061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
6121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
6181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
6241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
6301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
6361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
6421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
6481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
6541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
6601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
6661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
6721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
6781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
6841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
6901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
6961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
7021 cttttgctgg ccttttgctc acatgt 
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