pAAV-G-EF1a-hGH-amp

                            
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1405..1423
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    1426..1444
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     promoter        1607..1637
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
                     /note="This forward directional feature has 3 segments:
                        1: 1607 .. 1612 / #ffffff / -35
                        2: 1613 .. 1630 / #ffffff
                        3: 1631 .. 1637 / #ffffff / -10"
     CDS             1691..2350
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2692..2997
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="color: #993366"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     rep_origin      3918..4373
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4707..4811
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4812..5672
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4812 .. 4880 / #ccffcc / signal sequence
                        2: 4881 .. 5672 / #ccffcc
                     Cleavage site after base 4880"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5843..6431
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgccc taggggctcc ggtgcccgtc agtgggcaga gcgcacatcg
      181 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
      241 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      301 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      361 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      421 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
      481 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
      541 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      601 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
      661 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
      721 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
      781 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
      841 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
      901 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
      961 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     1021 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     1081 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     1141 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     1201 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1261 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     1321 tgagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     1381 tccatagaag aaccgagttt aaactcccta tcagtgatag agatctccct atcagtgata
     1441 gagagctagc gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     1501 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     1561 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     1621 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     1681 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1741 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1801 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1861 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1921 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1981 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2041 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2101 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     2161 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     2221 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     2281 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     2341 cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     2401 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     2461 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     2521 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     2581 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     2641 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     2701 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     2761 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     2821 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     2881 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     2941 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     3001 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     3061 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     3121 atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatatct gactcgagag
     3181 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     3241 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     3301 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     3361 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     3421 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     3481 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     3541 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3601 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3661 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3721 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3781 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3841 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3901 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3961 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     4261 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     4321 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4381 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4441 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4501 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4561 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4621 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4801 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4861 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4921 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4981 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5041 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5101 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5161 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5221 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5281 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5341 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5401 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5461 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5521 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5581 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5641 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5701 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5761 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6421 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6481 ttgctcacat gt
//