pAAV-G-PGK-saCas9-hGH-amp

                            LOCUS       dna                      7135 bp                                   
FEATURES             Location/Qualifiers
     misc_binding    149..154
                     /dbxref="REBASE:NdeI"
     ORF             332..3829
                     /sequence="ORF_2 rf(2)"
     ORF             497..3283
                     /sequence="ORF_3 rf(4)"
     misc_binding    554..559
                     /dbxref="REBASE:EcoRI"
     misc_binding    564..569
                     /dbxref="REBASE:NcoI"
     misc_binding    698..703
                     /dbxref="REBASE:ClaI"
     misc_binding    1969..1974
                     /dbxref="REBASE:HindIII"
     misc_binding    3821..3826
                     /dbxref="REBASE:BamHI"
     misc_binding    3830..3835
                     /dbxref="REBASE:XhoI"
     Terminator      3847..4323
                     /gene="hGH_PA term"
     Rep_Origin      4578..4884
                     /gene="f1 origin"
     Promoter        5385..5413
                     /gene="amp prom"
     Marker          5455..6315
                     /gene="amp marker"
     ORF             5455..6315
                     /sequence="ORF_1 rf(1)"
     Rep_Origin      6470..7089
                     /gene="pBR322 origin"
BASE COUNT    1899 a   1918 c   1894 g   1424 t    0 others
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt     
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact     
      121 aggggttcct gcggccgccc tagggcacca tatgcggtgt gaccggtagg cgccaaccgg     
      181 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc     
      241 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta     
      301 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg     
      361 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg     
      421 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc     
      481 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc     
      541 cgggcctttc gacgaattcg ccaccatggc cccaaagaag aagcggaagg tcggtatcca     
      601 cggagtccca gcagccaagc ggaactacat cctgggcctg gacatcggca tcaccagcgt     
      661 gggctacggc atcatcgact acgagacacg ggacgtgatc gatgccggcg tgcggctgtt     
      721 caaagaggcc aacgtggaaa acaacgaggg caggcggagc aagagaggcg ccagaaggct     
      781 gaagcggcgg aggcggcata gaatccagag agtgaagaag ctgctgttcg actacaacct     
      841 gctgaccgac cacagcgagc tgagcggcat caacccctac gaggccagag tgaagggcct     
      901 gagccagaag ctgagcgagg aagagttctc tgccgccctg ctgcacctgg ccaagagaag     
      961 aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc aacgagctgt ccaccaaaga     
     1021 gcagatcagc cggaacagca aggccctgga agagaaatac gtggccgaac tgcagctgga     
     1081 acggctgaag aaagacggcg aagtgcgggg cagcatcaac agattcaaga ccagcgacta     
     1141 cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc taccaccagc tggaccagag     
     1201 cttcatcgac acctacatcg acctgctgga aacccggcgg acctactatg agggacctgg     
     1261 cgagggcagc cccttcggct ggaaggacat caaagaatgg tacgagatgc tgatgggcca     
     1321 ctgcacctac ttccccgagg aactgcggag cgtgaagtac gcctacaacg ccgacctgta     
     1381 caacgccctg aacgacctga acaatctcgt gatcaccagg gacgagaacg agaagctgga     
     1441 atattacgag aagttccaga tcatcgagaa cgtgttcaag cagaagaaga agcccaccct     
     1501 gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat attaagggct acagagtgac     
     1561 cagcaccggc aagcccgagt tcaccaacct gaaggtgtac cacgacatca aggacattac     
     1621 cgcccggaaa gagattattg agaacgccga gctgctggat cagattgcca agatcctgac     
     1681 catctaccag agcagcgagg acatccagga agaactgacc aatctgaact ccgagctgac     
     1741 ccaggaagag atcgagcaga tctctaatct gaagggctat accggcaccc acaacctgag     
     1801 cctgaaggcc atcaacctga tcctggacga gctgtggcac accaacgaca accagatcgc     
     1861 tatcttcaac cggctgaagc tggtgcccaa gaaggtggac ctgtcccagc agaaagagat     
     1921 ccccaccacc ctggtggacg acttcatcct gagccccgtc gtgaagagaa gcttcatcca     
     1981 gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc ctgcccaacg acatcattat     
     2041 cgagctggcc cgcgagaaga actccaagga cgcccagaaa atgatcaacg agatgcagaa     
     2101 gcggaaccgg cagaccaacg agcggatcga ggaaatcatc cggaccaccg gcaaagagaa     
     2161 cgccaagtac ctgatcgaga agatcaagct gcacgacatg caggaaggca agtgcctgta     
     2221 cagcctggaa gccatccctc tggaagatct gctgaacaac cccttcaact atgaggtgga     
     2281 ccacatcatc cccagaagcg tgtccttcga caacagcttc aacaacaagg tgctcgtgaa     
     2341 gcaggaagaa aacagcaaga agggcaaccg gaccccattc cagtacctga gcagcagcga     
     2401 cagcaagatc agctacgaaa ccttcaagaa gcacatcctg aatctggcca agggcaaggg     
     2461 cagaatcagc aagaccaaga aagagtatct gctggaagaa cgggacatca acaggttctc     
     2521 cgtgcagaaa gacttcatca accggaacct ggtggatacc agatacgcca ccagaggcct     
     2581 gatgaacctg ctgcggagct acttcagagt gaacaacctg gacgtgaaag tgaagtccat     
     2641 caatggcggc ttcaccagct ttctgcggcg gaagtggaag tttaagaaag agcggaacaa     
     2701 ggggtacaag caccacgccg aggacgccct gatcattgcc aacgccgatt tcatcttcaa     
     2761 agagtggaag aaactggaca aggccaaaaa agtgatggaa aaccagatgt tcgaggaaaa     
     2821 gcaggccgag agcatgcccg agatcgaaac cgagcaggag tacaaagaga tcttcatcac     
     2881 cccccaccag atcaagcaca ttaaggactt caaggactac aagtacagcc accgggtgga     
     2941 caagaagcct aatagagagc tgattaacga caccctgtac tccacccgga aggacgacaa     
     3001 gggcaacacc ctgatcgtga acaatctgaa cggcctgtac gacaaggaca atgacaagct     
     3061 gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg taccaccacg acccccagac     
     3121 ctaccagaaa ctgaagctga ttatggaaca gtacggcgac gagaagaatc ccctgtacaa     
     3181 gtactacgag gaaaccggga actacctgac caagtactcc aaaaaggaca acggccccgt     
     3241 gatcaagaag attaagtatt acggcaacaa actgaacgcc catctggaca tcaccgacga     
     3301 ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg aagccctaca gattcgacgt     
     3361 gtacctggac aatggcgtgt acaagttcgt gaccgtgaag aatctggatg tgatcaaaaa     
     3421 agaaaactac tacgaagtga atagcaagtg ctatgaggaa gctaagaagc tgaagaagat     
     3481 cagcaaccag gccgagttta tcgcctcctt ctacaacaac gatctgatca agatcaacgg     
     3541 cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg aaccggatcg aagtgaacat     
     3601 gatcgacatc acctaccgcg agtacctgga aaacatgaac gacaagaggc cccccaggat     
     3661 cattaagaca atcgcctcca agacccagag cattaagaag tacagcacag acattctggg     
     3721 caacctgtat gaagtgaaat ctaagaagca ccctcagatc atcaaaaagg gcaaaaggcc     
     3781 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctgac tcgagccaat     
     3841 cttaaggggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc     
     3901 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag     
     3961 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg     
     4021 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa     
     4081 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc     
     4141 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag     
     4201 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac     
     4261 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc     
     4321 cttcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct     
     4381 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt     
     4441 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg     
     4501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt     
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg     
     4621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca     
     4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta     
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc     
     4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg     
     4861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat     
     4921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta     
     4981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct     
     5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct     
     5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     
     5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagccgggag ctgcatgtgt     
     5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta     
     5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg     
     5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg     
     5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt     
     5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt     
     5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg     
     5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa     
     5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt     
     5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     
     5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     
     5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     
     5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt     
     5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     
     6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     
     6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     
     6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     
     6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     
     6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     
     6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     
     6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     
     6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     
     6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     
     6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     
     6601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac     
     6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     
     6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     
     6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     
     6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     
     6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     
     6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     
     7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     
     7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt          
//