pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp
| Vector Name | pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | PGK Forward: AAT TCT ACC GGG TAG GGG AGG CGC T |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc taggaaaaaa atctcgccaa caagttgacg agataaacac 181 ggcattttgc cttgttttag tagattctgt ttccagagta ctaaaacggg tcttcccatg 241 ggaagacggc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt 301 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga 361 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta 421 attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga 481 aataggccct caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 541 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 601 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 661 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 721 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 781 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt 841 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacgaattcg ccaccatggc 901 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccaagc ggaactacat 961 cctgggcctg gacatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg 1021 ggacgtgatc gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg 1081 caggcggagc aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag 1141 agtgaagaag ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat 1201 caacccctac gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc 1261 tgccgccctg ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga 1321 ggacaccggc aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga 1381 agagaaatac gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg 1441 cagcatcaac agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt 1501 gcagaaggcc taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga 1561 aacccggcgg acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat 1621 caaagaatgg tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag 1681 cgtgaagtac gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt 1741 gatcaccagg gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa 1801 cgtgttcaag cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa 1861 cgaagaggat attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct 1921 gaaggtgtac cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga 1981 gctgctggat cagattgcca agatcctgac catctaccag agcagcgagg acatccagga 2041 agaactgacc aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct 2101 gaagggctat accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga 2161 gctgtggcac accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa 2221 gaaggtggac ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct 2281 gagccccgtc gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa 2341 gaagtacggc ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga 2401 cgcccagaaa atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga 2461 ggaaatcatc cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct 2521 gcacgacatg caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct 2581 gctgaacaac cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga 2641 caacagcttc aacaacaagg tgctcgtgaa gcaggaagaa aacagcaaga agggcaaccg 2701 gaccccattc cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa 2761 gcacatcctg aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct 2821 gctggaagaa cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct 2881 ggtggatacc agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt 2941 gaacaacctg gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg 3001 gaagtggaag tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct 3061 gatcattgcc aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa 3121 agtgatggaa aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac 3181 cgagcaggag tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt 3241 caaggactac aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga 3301 caccctgtac tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa 3361 cggcctgtac gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa 3421 gctgctgatg taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca 3481 gtacggcgac gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac 3541 caagtactcc aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa 3601 actgaacgcc catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa 3661 gctgtccctg aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt 3721 gaccgtgaag aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg 3781 ctatgaggaa gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt 3841 ctacaacaac gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa 3901 cgacctgctg aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga 3961 aaacatgaac gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag 4021 cattaagaag tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca 4081 ccctcagatc atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa 4141 aaagaaaaag ggatcctgac tcgagccaat cttaaggggt ggcatccctg tgacccctcc 4201 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata 4261 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg 4321 ggggtggtat ggagcaaggg gcaagttggg aagagaacct gtagggcctg cggggtctat 4381 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg 4441 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc 4501 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg 4561 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta 4621 caggcgtgaa ccactgctcc cttccctgtc cttcacgtgc ggaccgagcg gccgcaggaa 4681 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 4741 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 4801 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 4861 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg 4921 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc 4981 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 5041 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 5101 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 5161 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 5221 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt 5281 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 5341 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 5401 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 5461 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 5521 cgaaacgcgc gagccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 5581 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 5641 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 5701 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 5761 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 5821 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 5881 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 5941 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 6001 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 6061 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 6121 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 6181 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 6241 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 6301 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 6361 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 6421 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 6481 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 6541 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 6601 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 6661 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 6721 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 6781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 6841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 6901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 6961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 7021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 7081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 7141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 7201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 7261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 7321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 7381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 7441 ttttgctggc cttttgctca catgt
FEATURES Location/Qualifiers
source 1..7465
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
misc_RNA complement(152..227)
/note="Sa gRNA scaffold"
/note="color: #00ccff; direction: LEFT"
promoter complement(251..491)
/note="U6 promoter"
/note="color: #ffffff; direction: LEFT"
promoter 493..883
/note="PGK"
/note="color: #ffffff; direction: RIGHT"
CDS 902..922
/codon_start=1
/note="SV40 NLS"
/note="color: #993366"
/translation="PKKKRKV"
CDS 947..4102
/codon_start=1
/note="SaCas9"
/note="color: #993366"
/translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
IKKG"
CDS 4103..4150
/codon_start=1
/note="nucleoplasmin NLS"
/note="color: #993366"
/translation="KRPAATKKAGQAKKKK"
polyA_signal 4177..4653
/note="hGH poly(A) signal"
/note="color: #a6acb3"
repeat_region 4676..4816
/note="AAV2 ITR"
/note="color: #ffe4c4"
repeat_region 4676..4805
/note="AAV2 ITR"
/note="color: #ffcc99"
rep_origin 4891..5346
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 5680..5784
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 5785..6645
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
1: 5785 .. 5853 / #ccffcc / signal sequence
2: 5854 .. 6645 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6816..7404
/direction=RIGHT
/note="ori"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggaaaaaa atctcgccaa caagttgacg agataaacac
181 ggcattttgc cttgttttag tagattctgt ttccagagta ctaaaacggg tcttcccatg
241 ggaagacggc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
301 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
361 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
421 attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
481 aataggccct caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
541 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
601 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
661 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
721 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
781 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
841 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacgaattcg ccaccatggc
901 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccaagc ggaactacat
961 cctgggcctg gacatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg
1021 ggacgtgatc gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg
1081 caggcggagc aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag
1141 agtgaagaag ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat
1201 caacccctac gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc
1261 tgccgccctg ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga
1321 ggacaccggc aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga
1381 agagaaatac gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg
1441 cagcatcaac agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt
1501 gcagaaggcc taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga
1561 aacccggcgg acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat
1621 caaagaatgg tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag
1681 cgtgaagtac gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt
1741 gatcaccagg gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa
1801 cgtgttcaag cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa
1861 cgaagaggat attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct
1921 gaaggtgtac cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga
1981 gctgctggat cagattgcca agatcctgac catctaccag agcagcgagg acatccagga
2041 agaactgacc aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct
2101 gaagggctat accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga
2161 gctgtggcac accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa
2221 gaaggtggac ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct
2281 gagccccgtc gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa
2341 gaagtacggc ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga
2401 cgcccagaaa atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga
2461 ggaaatcatc cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct
2521 gcacgacatg caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct
2581 gctgaacaac cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga
2641 caacagcttc aacaacaagg tgctcgtgaa gcaggaagaa aacagcaaga agggcaaccg
2701 gaccccattc cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa
2761 gcacatcctg aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct
2821 gctggaagaa cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct
2881 ggtggatacc agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt
2941 gaacaacctg gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg
3001 gaagtggaag tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct
3061 gatcattgcc aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa
3121 agtgatggaa aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac
3181 cgagcaggag tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt
3241 caaggactac aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga
3301 caccctgtac tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa
3361 cggcctgtac gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa
3421 gctgctgatg taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca
3481 gtacggcgac gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac
3541 caagtactcc aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa
3601 actgaacgcc catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa
3661 gctgtccctg aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt
3721 gaccgtgaag aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg
3781 ctatgaggaa gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt
3841 ctacaacaac gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa
3901 cgacctgctg aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga
3961 aaacatgaac gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag
4021 cattaagaag tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca
4081 ccctcagatc atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
4141 aaagaaaaag ggatcctgac tcgagccaat cttaaggggt ggcatccctg tgacccctcc
4201 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
4261 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
4321 ggggtggtat ggagcaaggg gcaagttggg aagagaacct gtagggcctg cggggtctat
4381 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
4441 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
4501 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
4561 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
4621 caggcgtgaa ccactgctcc cttccctgtc cttcacgtgc ggaccgagcg gccgcaggaa
4681 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4741 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4801 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4861 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4921 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4981 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
5041 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
5101 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
5161 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
5221 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
5281 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
5341 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5401 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5461 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5521 cgaaacgcgc gagccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
5581 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
5641 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
5701 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
5761 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
5821 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5881 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5941 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
6001 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
6061 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
6121 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
6181 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
6241 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
6301 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
6361 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
6421 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
6481 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
6541 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
6601 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
6661 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
6721 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
6781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
7021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
7081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
7141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
7201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
7261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
7321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
7381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
7441 ttttgctggc cttttgctca catgt
//