pAAV-U6-sgRNAsa-GFP-hGH-amp
| Vector Name | pAAV-U6-sgRNAsa-GFP-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Amp |
| Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc tagggggtag gggaggcgct tttcccaagg cagtctggag 181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca 241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc 301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca 361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg 421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt 481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg 541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca 601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcggctagc acctcgccac 661 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 721 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 781 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 841 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 901 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 961 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 1021 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 1081 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1141 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1201 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1261 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1321 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 1381 actcgagctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1561 gaggattggg aagagaatag caggcatgct ggggacaatt ggagggccta tttcccatga 1621 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga 1681 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt 1741 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa 1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgccgtctt cccatgggaa 1861 gacccgtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg ccgtgtttat 1921 ctcgtcaact tgttggcgag attttttttc tagacttaag gggtggcatc cctgtgaccc 1981 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct 2041 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg 2101 gaggggggtg gtatggagca aggggcaagt tgggaagaga acctgtaggg cctgcggggt 2161 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc 2221 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat 2281 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag 2341 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg 2401 attacaggcg tgaaccactg ctcccttccc tgtccttcac gtgcggaccg agcggccgca 2461 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 2521 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 2581 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg 2641 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc 2701 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc 2761 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2821 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2881 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2941 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 3001 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat 3061 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 3121 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 3181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 3241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 3301 tcaccgaaac gcgcgagccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 3361 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 3421 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 3481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 3541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 3601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 3661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 3721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 3781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 3841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 3901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 3961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 4021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 4081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 4141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 4201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 4261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 4321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 4381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 4441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 4501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 4561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 4621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 4681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 4741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 4801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 4861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 4921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 5041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 5101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 5161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 5221 ggccttttgc tggccttttg ctcacatgt
FEATURES Location/Qualifiers
source 1..5249
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 145..644
/note="PGK promoter"
/note="phosphoglycerate kinase promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 662..1381
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/note="This feature has 3 segments:
1: 662 .. 664 / #05fd14
2: 665 .. 667 / #05fd14 / 1a
3: 668 .. 1381 / #05fd14"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
rep_origin 2675..3130
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 3464..3568
/gene="bla"
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 3569..4429
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 3569 .. 3637 / #ccffcc / signal sequence
2: 3638 .. 4429 / #ccffcc
Cleavage site after base 3637"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4600..5188
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc tagggggtag gggaggcgct tttcccaagg cagtctggag
181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcggctagc acctcgccac
661 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
721 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
781 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
841 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
901 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
961 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
1021 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
1081 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
1141 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
1201 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
1261 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
1321 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
1381 actcgagctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1561 gaggattggg aagagaatag caggcatgct ggggacaatt ggagggccta tttcccatga
1621 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
1681 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
1741 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgccgtctt cccatgggaa
1861 gacccgtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg ccgtgtttat
1921 ctcgtcaact tgttggcgag attttttttc tagacttaag gggtggcatc cctgtgaccc
1981 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
2041 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
2101 gaggggggtg gtatggagca aggggcaagt tgggaagaga acctgtaggg cctgcggggt
2161 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
2221 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
2281 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
2341 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
2401 attacaggcg tgaaccactg ctcccttccc tgtccttcac gtgcggaccg agcggccgca
2461 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
2521 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
2581 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
2641 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
2701 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
2761 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
2821 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
2881 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
2941 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
3001 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
3061 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
3121 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
3181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
3241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
3301 tcaccgaaac gcgcgagccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
3361 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
3421 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
3481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
3541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
3601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
3661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
3721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
3781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
3841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
3901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
3961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
4021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
4081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
4141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
4201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
4261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
4321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
4381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
4441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
4501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
4561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
4621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
4681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
4741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
4801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
4861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
4921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
4981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
5041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
5101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
5161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
5221 ggccttttgc tggccttttg ctcacatgt
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