pAAV-U6-sgRNAsp-GFP-hGH-amp
| Vector Name | pAAV-U6-sgRNAsp-GFP-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Amp |
| Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc tagggggtag gggaggcgct tttcccaagg cagtctggag 181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca 241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc 301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca 361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg 421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt 481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg 541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca 601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcggctagc acctcgccac 661 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 721 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 781 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 841 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 901 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 961 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 1021 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 1081 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1141 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1201 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1261 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1321 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 1381 actcgagctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1561 gaggattggg aagagaatag caggcatgct ggggacaatt ggagggccta tttcccatga 1621 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga 1681 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt 1741 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa 1801 agtatttcga tttcttgggt ttatatatct tgtggaaagg acgaaacacc ggagtcttcc 1861 catgggaaga cgcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc 1921 aacttgaaaa agtggcaccg agtcggtgct tttttttatc ggatctgata tctctagact 1981 taaggggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac 2041 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt 2101 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa 2161 gagaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc 2221 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga 2281 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag 2341 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc 2401 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct 2461 tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 2521 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 2581 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 2641 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 2701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2761 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2881 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 2941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3001 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa 3061 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3121 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 3181 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 3241 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 3301 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gccgggagct gcatgtgtca 3361 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt 3421 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 3481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 3601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 3841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 3901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 4021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 4081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 4141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 4201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 4261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 4321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 4381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 4441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 4501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 4801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 5041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 5101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 5161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 5221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
FEATURES Location/Qualifiers
source 1..5273
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 145..644
/note="PGK promoter"
/note="phosphoglycerate kinase promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 662..1381
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/note="This feature has 3 segments:
1: 662 .. 664 / #05fd14
2: 665 .. 667 / #05fd14 / 1a
3: 668 .. 1381 / #05fd14"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
rep_origin 2699..3154
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 3488..3592
/gene="bla"
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 3593..4453
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 3593 .. 3661 / #ccffcc / signal sequence
2: 3662 .. 4453 / #ccffcc
Cleavage site after base 3661"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4624..5212
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc tagggggtag gggaggcgct tttcccaagg cagtctggag
181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcggctagc acctcgccac
661 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
721 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
781 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
841 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
901 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
961 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
1021 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
1081 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
1141 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
1201 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
1261 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
1321 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
1381 actcgagctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1561 gaggattggg aagagaatag caggcatgct ggggacaatt ggagggccta tttcccatga
1621 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
1681 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
1741 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
1801 agtatttcga tttcttgggt ttatatatct tgtggaaagg acgaaacacc ggagtcttcc
1861 catgggaaga cgcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
1921 aacttgaaaa agtggcaccg agtcggtgct tttttttatc ggatctgata tctctagact
1981 taaggggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
2041 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
2101 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
2161 gagaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
2221 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
2281 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
2341 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
2401 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
2461 tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
2521 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
2581 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
2641 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
2701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
2761 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
2821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
2881 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
2941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
3001 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
3061 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
3121 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
3181 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
3241 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
3301 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gccgggagct gcatgtgtca
3361 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
3421 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
3481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
3601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
3661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
3721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
3781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
3841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
3901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
3961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
4021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
4081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
4141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
4201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
4261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
4321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
4381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
4441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
4501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
4561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
4621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
4681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
4741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
4801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
4861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
4921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
4981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
5041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
5101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
5161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
5221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
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