pAAV-shRNA-GFP-hGH-amp

                            
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gap             413..433
                     /estimated_length="21"
                     /note="color: #------"
     gap             438..458
                     /estimated_length="21"
                     /note="color: #------"
     enhancer        524..829
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        830..1033
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1088..1804
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1088 .. 1090 / #05fd14
                        2: 1091 .. 1093 / #05fd14 / 1a
                        3: 1094 .. 1804 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1886..2488
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     rep_origin      3209..3664
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3998..4102
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4103..4963
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4103 .. 4171 / #ccffcc / signal sequence
                        2: 4172 .. 4963 / #ccffcc
                     Cleavage site after base 4171"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5134..5722
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgccc taggtacgtc caaggtcggg caggaagagg gcctatttcc
      181 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
      241 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
      301 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
      361 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacaaa aannnnnnnn
      421 nnnnnnnnnn nnncgaannn nnnnnnnnnn nnnnnnnntt ttttggtacc aattgtagta
      481 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      541 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      601 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      661 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      721 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      781 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      841 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      901 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      961 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1021 tataagcaga gctggtttag tgaaccgtca gggtaccgga tcccttaagt gaacaactag
     1081 tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1201 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1261 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1321 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1561 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1681 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1801 caaggaattc ggaagcgggc agtgcaccaa ctacgccctg ctgaagctgg ccggcgacgt
     1861 ggagagcaac cccggccccg gatccatggc caccgagtac aagcccacgg tgcgcctcgc
     1921 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     1981 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2041 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2101 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2161 cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2221 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2281 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2341 cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2401 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     2461 gtgcatgacc cgcaagcccg gtgcctgaac gcgtgggtgg catccctgtg acccctcccc
     2521 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     2581 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     2641 ggtggtatgg agcaaggggc aagttgggaa gagaacctgt agggcctgcg gggtctattg
     2701 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     2761 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     2821 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     2881 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     2941 ggcgtgaacc actgctccct tccctgtcct tcacgtgcgg accgagcggc cgcaggaacc
     3001 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3061 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3121 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3181 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3241 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3301 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3361 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3421 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3481 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3541 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3601 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3661 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3721 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3781 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3841 aaacgcgcga gccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3901 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3961 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4021 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4081 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4141 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4201 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4261 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4321 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4381 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4441 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4501 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4561 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4621 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4681 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4741 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4801 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4861 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4921 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4981 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5041 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5101 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5161 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5221 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5281 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5341 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5401 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5461 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5521 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5581 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5641 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5701 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5761 ttgctggcct tttgctcaca tgt
//