pAAV-siRNA-GFP-hGH-amp

                            
LOCUS       Exported File           5999 bp ds-DNA    circular SYN 25-FEB-2025
 
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-siRNA-GFP-hGH-amp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
 
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /note="AAV2 ITR (alternate)"
                     /note="color: #ffcc99"
     promoter        167..407
                     /note="U6 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     gap             413..441
                     /estimated_length="29"
                     /note="color: #------"
     promoter        complement(470..684)
                     /note="H1 promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        742..1045
                     /note="CMV enhancer"
                     /note="color: #ffffff"
     promoter        1046..1249
                     /note="CMV promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1304..2020
                     /codon_start=1
                     /note="EGFP"
                     /note="This feature has 3 segments:
                        1: 1304 .. 1306 / #05fd14
                        2: 1307 .. 1309 / #05fd14 / 1a
                        3: 1310 .. 2020 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2036..2095
                     /codon_start=1
                     /note="E2A"
                     /note="color: #cc99b2
                     Cleavage site after base 2092"
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             2102..2704
                     /codon_start=1
                     /note="PuroR"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2711..3187
                     /note="hGH poly(A) signal"
                     /note="color: #a6acb3"
     misc_feature    2889
                     /note="BBsI destroyed"
                     /note="color: #a6acb3"
     repeat_region   3210..3350
                     /note="AAV2 ITR"
                     /note="color: #ffe4c4"
     repeat_region   3210..3339
                     /note="AAV2 ITR"
                     /note="color: #ffcc99"
     rep_origin      3425..3880
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="color: #ffff00"
     promoter        4214..4318
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4319..5179
                     /codon_start=1
                     /note="AmpR"
                     /note="This feature has 2 segments:
                        1: 4319 .. 4387 / #ccffcc / signal sequence
                        2: 4388 .. 5179 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5350..5938
                     /direction=RIGHT
                     /note="ori"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgccc taggtacgtc caaggtcggg caggaagagg gcctatttcc
      181 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
      241 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
      301 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
      361 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacaaa aannnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nttttttggt accctttttt tggtgtttcg tggtctcata
      481 cagaacttat aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac
      541 acatagcgac atgcaaatat tgcagggcgc cactcccctg tccctcacag ccatcttcct
      601 gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc gattccttgg
      661 agcgggttga tgacgtcagc gttctcaatt gtagtaatca attacggggt cattagttca
      721 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      781 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      841 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      901 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      961 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1021 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1081 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1141 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1201 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     1261 ccgtcagggt accggatccc ttaagtgaac aactagtgcc accatggtga gcaagggcga
     1321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1441 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1501 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1741 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1801 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1861 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1921 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1981 cgccgccggg atcactctcg gcatggacga gctgtacaag gaattcggaa gcgggcagtg
     2041 caccaactac gccctgctga agctggccgg cgacgtggag agcaaccccg gccccggatc
     2101 catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     2161 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     2221 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     2281 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     2341 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag
     2401 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     2461 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     2521 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt
     2581 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2641 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2701 ctgaacgcgt gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     2761 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     2821 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     2881 tgggaagaga acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     2941 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     3001 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     3061 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     3121 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     3181 tgtccttcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     3241 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3301 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3361 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3421 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3481 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     3541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3721 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     3781 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     3841 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     3901 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3961 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4021 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagccg ggagctgcat
     4081 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4141 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//