pHSV-CMV-Neo
| Vector Name | pHSV-CMV-Neo |
| VectorType | Herpes Simplex Virus (HSV) Plasmid |
| Antibiotic Information | Bacterial: Ampicillin. Mammalian: Neomycin. |
| Sequencing Primers | CMV sequencing primer: 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer: 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Vector Size without Insert: 6055bp |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 gatctgcctg caggcatgca agctgtaccc ggggccggcg cggagtcggg cacggcgcca
61 gtgctcgcac ttcgccctaa taatatatat atattgggac gaagtgcgaa cgcttcgcgt
121 tctcacttct tttacccggc ggccccgccc ccttggggcg gtcccgcccg ccggccaatg
181 ggggggcggc aaggcgggcg gcccttgggc cgcccgccgt cccgttggtc ccaacgtccg
241 gcgggcggga ccgggggccc ggggatcccc ggccgcgggg ggcccgggct gcgccgccgc
301 gctttaaagg gccgcgcgcg acccccgggg ggtgtgtttt ggggggggcc cgttttcggc
361 gtctggccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
421 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
481 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
541 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctccc gcggccccgc
601 cccccacgcc cgccgcgcgc gcgcacgccg cccggaccgc cgcccgcctt ttttgcgcgc
661 cgccccgccc gcggggggcc cgggctgcca caggtgtaac cccgggaatt agcttggcac
721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
781 ttgcagcaca tccccctttc gccagcgatg tacgggccag atatacgcgt tgacattgat
841 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
1021 gacgtcaatg ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
1081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
1141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
1201 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
1261 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
1321 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
1381 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
1441 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
1501 ctcggatcca ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg
1561 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
1621 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
1681 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
1741 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
1801 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
1861 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
1921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
1981 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
2041 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
2101 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
2161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
2221 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
2281 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
2341 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
2401 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
2521 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
2581 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
2641 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
2701 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
2761 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
2821 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
2881 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
2941 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
3001 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
3061 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
3121 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
3181 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
3241 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
3301 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
3361 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
3421 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
3481 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
3541 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
3601 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
3661 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
3721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
3781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
3841 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
3901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
3961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
5761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
5821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
5881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cggga
LOCUS dna 6055 bp
FEATURES Location/Qualifiers
ORF 16..699
/sequence="ORF_1 rf(1)"
misc_binding 1080..1085
/dbxref="REBASE:NdeI"
Promoter 1460..1478
/gene="T7 prom"
misc_binding 1486..1491
/dbxref="REBASE:HindIII"
misc_binding 1492..1497
/dbxref="REBASE:KpnI"
misc_binding 1535..1540
/dbxref="REBASE:EcoRI"
misc_binding 1545..1550
/dbxref="REBASE:EcoRV"
misc_binding 1561..1568
/dbxref="REBASE:NotI"
misc_binding 1568..1573
/dbxref="REBASE:XhoI"
misc_binding 1580..1585
/dbxref="REBASE:XbaI"
Promoter 1597..1614
/gene="Sp6 prom"
Rep_Origin 1926..2232
/gene="f1 origin"
Regulatory_Seq 2342..2578
/gene="SV40ER reg"
Promoter 2364..2633
/gene="SV40 prom"
Promoter 2485..2686
/gene="SV40 prom"
Rep_Origin 2532..2609
/gene="SV40 origin"
misc_binding 2663..2668
/dbxref="REBASE:StuI"
ORF 2748..3542
/sequence="ORF_2 rf(3)"
Marker 2751..3539
/gene="NTP_II marker"
Promoter 3935..3964
/gene="lac prom"
Rep_Origin 4273..4892
/gene="pBR322 origin"
misc_binding 4638..4646
/dbxref="REBASE:AlwNI"
Marker 5047..5907
/gene="amp marker"
ORF 5047..5907
/sequence="ORF_3 rf(5)"
Promoter 5949..5977
/gene="amp prom"